5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB) Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored…

Features Synonym: Homidium Bromide CAS#: 1239-45-8 Molecular Formula: C₂₁H₂₀BrN₃ Molecular Weight: 394.294    

N -(ε-Maleimidocaproic acid) hydrazide Features Molecular Weight: 225.24 Spacer Arm (Å): 11.8 Reactive Toward: Sulfhydryl + Carbohydrate Membrane Permeable: Not tested Water Soluble: NO Cleavable/ Reversible: NO

N -(ε-Maleimidocaproyloxy) succinimide ester Features Molecular Weight: 308.29 Spacer Arm (Å): 9.4 Reactive Toward: Primary Amine + Sulfhydryl Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: NO

Also available as EDTA, disodium salt and EDTA Features Synonym: Ethylenediaminetetraacetic acid CAS#: 6381-92-6 Molecular Formula: C₁₀H₁₆N₂O₈ Molecular Weight: 292.24

Ethylene glycol bis (succinimidyl succinate) Ideal for receptor ligand crosslinking Features Molecular Weight: 456.36 Spacer Arm (Å): 16.1 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: Hydroxylamine

EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and…