Collagenase Type 3 is lower in secondary proteolytic contaminant activities but with typical collagenase activity. It is suggested for mammary primary cell isolation. Collagenase is typically used at concentrations from 0.05 % to 0.5 % (w/v) in balanced salt solutions such as Hank’s, Earle’s and…

Collagenase Type 4 contain lower tryptic activity levels to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. It is suggested for pancreatic islet primary isolation. Collagenase is typically used at concentrations from 0.05 % to 0.5 % (w/v) in…

Elastase is assayed using a method adapted from that of Feinstein et al., Biochem. Biophys. Res. Comm., 50, 1020 (1973) and using the more soluble substrate of Bieth et al., Biochem. Med., 11, 350 (1974). 1 SucAla3NA unit is approximately equivalent to 6 elastin digestion units. Aqueous liquid…

Buffy coat preparations are suitable for use as a source of nucleated PBMC. Buffy coat preparations are separated after a single low-speed centrifugation to remove the platelet rich plasma, buffy coat layer and erythrocytes (RBC). Buffy coat preparations are generally enriched for leukocytes…

Collagenase Type 1 is suggested for epithelial, liver, lung and adrenal primary cell isolations. Collagenase is typically used at concentrations from 0.05 % to 0.5 % (w/v) in balanced salt solutions such as Hank’s, Earle’s and others. For best results the precise mixture of collagenase and…

Collagenase Type 1 is suggested for epithelial, liver, lung and adrenal primary cell isolations. Collagenase is typically used at concentrations from 0.05 % to 0.5 % (w/v) in balanced salt solutions such as Hank’s, Earle’s and others. For best results the precise mixture of collagenase and…

Collagenase Type 2 contain higher clostripain activity. This is suggested for bone, heart, liver, thyroid and salivary primary cell isolation. Collagenase is typically used at concentrations from 0.05 % to 0.5 % (w/v) in balanced salt solutions such as Hank’s, Earle’s and others. For best results…