Isolation & Purification

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Expedeon Protein Isolation & Purification

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  • RunBlue™ SDS Run Buffer

    Expedeon

    RunBlue SDS Running Buffer is optimised for use with RunBlue Precast Gels to provide the best running conditions and optimal sample resolution. Based on a TEO-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high…

  • RunBlue™ Rapid SDS Run Buffer

    Expedeon

    RunBlue RAPID SDS RunBuffer is optimised for use with RunBlue Precast Gels to provide the best running contitions and optimal sample resolution. Based on a Tris-MOPS buffer system this run buffer provides excellent band resolution and fast run time. A 10% RunBlue gel will be run to completion…

  • RunBlue™ Run & Blot Systems

    Expedeon

    Expedeon's RunBlue Run & Blot system provides you with a gel tank with unsurpassed functionality. The core module hinges open to accept gel cassettes or buffer dam and then simply clips in place to provide a completely sealed cathode chamber. This allows RunBuffer to be added and samples to…

  • Amintra Protein A Resin

    Expedeon

    Amintra Protein A resins are designed on a highly crosslinked sepharose matrix covalently modified with recombinant Protein A and Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with high capacity and affinity for antibodies.

  • TEV-Express

    Expedeon

    TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express does not require any special buffers for its activity and can…

  • pIMAGO-biotin

    Expedeon

    pIMAGO is a universal phosphoprotein detection technology that enables sensitive and specific recognition of phosphorylated molecules. Unlike phospho-antibodies binding is not biased by amino acid sequence, and can therefore be used for detection of any phosphorylation event on any protein site.…

  • Amintra NI-NTA Resin

    Expedeon

    Expedeon's Ni-NTA affinity resin is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Amintra Ni-NTA offers excellent flow properties and high protein binding capacity: in excess of 50mg protein/ml. NTA is a…

  • RunBlue™ Native Run Buffer

    Expedeon

    RunBlue Native RunBuffer is optimised for use with RunBlue Precast Gels to provide the best running contitions and optimal sample resolution. Based on a Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high moleculr…

  • FFPE-FASP™ Protein Digestion Kit

    Expedeon

    Filter-Aided Sample Prep (FASP) is the enabling technology behind quantitative mass spectrometry analysis of archived tissues. The resulting filtrate is free of detergents, large molecules, and other substances that would interfere with mass spectrometry analysis of the proteome. Contents:…

  • Amintra Streptavidin Resin

    Expedeon

    Amintra Streptavidin Resin offers an ultra-high biotin-binding capacity with a very low leaching.  Constituted of pure recombinant streptavidin covalently cross-linked and immobilised on fine beaded agarose, Amintra Streptavidin Resin can be used to bind biotinylated biomolecules in a…

  • Amintra Glutathione Magnetic Resin for GST-Tagged Proteins

    Expedeon

    GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single purification step. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in…

  • Amintra Nickel Magnetic Resin for His-Tagged Proteins

    Expedeon

    His-tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing…

  • Amintra Protein L Resin

    Expedeon

    Protein L is an immunoglobulin-binding protein that was expressed, isolated from the bacterial species Peptostreptococcus magnus.  Protein L Agarose resin offers a unique ability in a convenient way to separate a wider range of mammalian Immunoglobulin classes and sub-classes compare to…

  • Amintra Glutathione Resin

    Expedeon

    Amintra's Glutathione resin is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to a high crosslinked agarose matrix that provides a high chemical and physical stability…

  • Amintra Protein A Magnetic Resin

    Expedeon

    Protein A is immobilised to highly porous and highly cross-linked agarose incorporating magnetic material. The particles have an open pore structure with excellent mass transfer properties for large protein molecules. Amintra Protein A Magnetic can be used in any commercially available magnetic…

  • NVoy Polymers

    Expedeon

    NVoy is a large, uncharged polymer based on a polysaccharide backbone with selected hydrophobic side chains substituted onto the backbone at specific residues to create an amphipathic molecule. NVoy is used in place of detergents, fusion proteins, arginine, chaperones and other common additives…

  • GELFREE® 8100 Fractionation Station

    Expedeon

    The compact, bench-top Gelfree 8100 Fractionation Station provides specified voltage to each of eight independent channels and pauses for fraction collection according to user programming. Using the touch screen interface, the fractionation sequence is defined or selected, permitting broad mass…

  • Amintra Heparin Resin

    Expedeon

    Amintra Heparin Resin is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as antithrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on…

  • Stabil-PAC

    Expedeon

    Each Stabil-PAC kit contains NVoy technology designed to increase the solubility and stability of proteins whilst preventing aggregation and reducing non-specific binding. Stabil-PAC kits are a convenient way to test if NVoy is able to assist your work. These packs contain single shot amounts of…

  • PolyMAC

    Expedeon

    Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful phosphoproteomics experiments. The products described here, termed PolyMAC (Polymer-based Metal ion Affinity Capture), overcome the shortcomings of current enrichment methods that typically lack…

  • GELFREE® 8100 Cartridge Kits

    Expedeon

    Ready-to-use Gelfree 8100 Cartridge Kits complement the Gelfree 8100 Fractionation Work Station. Each Cartridge Kit contains pre-formulated Running and Sample Buffers, as well as the Fractionation Cartridge. The Gelfree 8100 Fractionation Cartridge contains eight independent channels for molecular…

  • RunBlue™ Gel Drying Solution

    Expedeon

  • RunBlue™ Blot Membranes

    Expedeon

    Blot Membranes for use in Dual Run & Blot system. Each membrane is sandwiched between two thick pieces of blotting paper for protection which are further separated by blue paper inserts for easy identification of individual blot sandwiches. Pore size - 0.45 µm.

  • UPX™ Universal Protein Extraction Kit

    Expedeon

    The UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, and an abundance of starting material. One Protein Extraction Protocol for Tissues and Cells, Fresh or Frozen Expedeon's UPX Protein…

  • RunBlue™ LDS Sample Buffer

    Expedeon

    RunBlue LDS Sample Buffer should be used to prepare all samples for running on RunBlue Precast Gels. Specifically formulated for the RunBlue buffer system to provide the best running conditions and results.

  • RunBlue™ Native Sample Buffer

    Expedeon

    All RunBlue Buffers, Reagents and Accessories have been specifically designed and formulated for use with the RunBlue gels and apparatus to provide optimum performance and results. Buffers are made using the highest quality chemicals and have different compositions to other manufacturers buffers.

  • Amintra Protein G Resin

    Expedeon

    Amintra Protein G resin; a fast flow sepharose matrix covalently modified with recombinant Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with a high capacity and affinity for antibodies. Typically binding capacity of 25 mg…

  • Amintra NHS-Activated Resin

    Expedeon

    Amintra NHS-activated is a highly cross-linked N-hydroxysuccinimide activated matrix. Ligands containing primary amino groups are coupled directly to this active ester to form a chemically stable amide bond.

  • FASP™ Protein Digestion Kit

    Expedeon

    Complete Protein Solubilization. Complete Trypsin Digestion. Every Time. Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample…

  • RunBlue™ DTT Reducer

    Expedeon

    RunBlue DTT Reducer is a reducing agent which should be added during sample preparation if you wish to perform reduced SDS-PAGE.

  • YPX™ Yeast Protein Extraction Kit

    Expedeon

    A Simplified Protocol for Higher Yield Extraction Any Morphology. Any Medium. Complete Solubilization. Expedeon's YPX Yeast Protein Extraction Kit is for researchers who need fast, high-yield, unbiased yeast protein extraction and the ability to handle many samples at once. The…

  • RunBlue™ Tris-Glycine Blot Buffer

    Expedeon

    RunBlue Tris-Glycine Blotting Buffer is formulated for use with Expedeon Precast SDS-PAGE Gels. Dilution of RunBlue TG Buffer varies between the various blotting aparatuses.

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