Isolation & Purification

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Protein Isolation & Purification

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  • Amylose Resin

    Zymo Research Corporation

    The Amylose Resin is used for purification of MBP-tagged proteins from cell-free extracts The Amylose Resin is an affinity matrix which specifically binds proteins fused to maltose-binding protein (MBP). When using the Amylose Resin with the MBP-Spin Protein Miniprep Kits, proteins can be…

  • GELFREE® 8100 Fractionation Station

    Expedeon

    The compact, bench-top Gelfree 8100 Fractionation Station provides specified voltage to each of eight independent channels and pauses for fraction collection according to user programming. Using the touch screen interface, the fractionation sequence is defined or selected, permitting broad mass…

  • Amintra NHS-Activated Resin

    Expedeon

    Amintra NHS-activated is a highly cross-linked N-hydroxysuccinimide activated matrix. Ligands containing primary amino groups are coupled directly to this active ester to form a chemically stable amide bond.

  • pIMAGO-biotin

    Expedeon

    pIMAGO is a universal phosphoprotein detection technology that enables sensitive and specific recognition of phosphorylated molecules. Unlike phospho-antibodies binding is not biased by amino acid sequence, and can therefore be used for detection of any phosphorylation event on any protein site.…

  • RunBlue™ SDS Run Buffer

    Expedeon

    RunBlue SDS Running Buffer is optimised for use with RunBlue Precast Gels to provide the best running conditions and optimal sample resolution. Based on a TEO-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high…

  • Strep-Spin Protein Miniprep Kits

    Zymo Research Corporation

    The Strep-Spin Protein Miniprep Kit is based on a novel Strep-Tactin XT Superflow resin which binds to Twin-Strep-tag with very high affinity. This tag contains two Strep-tag II peptides connected via a Glycine/Serine linker sequence. Hence this kit is optimized for the purification of…

  • RunBlue™ DTT Reducer

    Expedeon

    RunBlue DTT Reducer is a reducing agent which should be added during sample preparation if you wish to perform reduced SDS-PAGE.

  • UPX™ Universal Protein Extraction Kit

    Expedeon

    The UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, and an abundance of starting material. One Protein Extraction Protocol for Tissues and Cells, Fresh or Frozen Expedeon's UPX Protein…

  • Amintra Streptavidin Resin

    Expedeon

    Amintra Streptavidin Resin offers an ultra-high biotin-binding capacity with a very low leaching.  Constituted of pure recombinant streptavidin covalently cross-linked and immobilised on fine beaded agarose, Amintra Streptavidin Resin can be used to bind biotinylated biomolecules in a…

  • Yeast Protein Kit

    Zymo Research Corporation

    The Yeast Protein Kit is a simple and convenient method for the rapid, thorough lysis of yeast cells. The kit has been optimized for use with S. cerevisiae and C. albicans but can be used for any fungal species that is susceptible to yeast lytic enzyme (Zymolyase) digestion. The digestion procedure…

  • Amintra Nickel Magnetic Resin for His-Tagged Proteins

    Expedeon

    His-tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing…

  • 2D-Xtract™

    G-Biosciences

    A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native charge of the proteins, due to carbamylation.Urea…

  • Amintra NI-NTA Resin

    Expedeon

    Expedeon's Ni-NTA affinity resin is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Amintra Ni-NTA offers excellent flow properties and high protein binding capacity: in excess of 50mg protein/ml. NTA is a…

  • RunBlue™ Gel Drying Solution

    Expedeon

  • NVoy Polymers

    Expedeon

    NVoy is a large, uncharged polymer based on a polysaccharide backbone with selected hydrophobic side chains substituted onto the backbone at specific residues to create an amphipathic molecule. NVoy is used in place of detergents, fusion proteins, arginine, chaperones and other common additives…

  • Protein Precipitation Kit

    Amresco

    Removal of interfering salts, detergents, and buffers Reproducible and consistent results This Protein Precipitation Kit utilizes a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and…

  • Classical Immunoprecipitation Kit

    G-Biosciences

    Immunoprecipitation (IP) is one of the most useful immunochemical techniques.IPs are routinely used to determinethe presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interactions with other…

  • RunBlue™ Antioxidant

    Expedeon

    RunBlue Antioxidant is recommended for use when running reduced samples to provide the best possible resolution of bands. RunBlue Antioxidant runs ahead of the dye front ensuring that the environment through which the protein is migrating is fully reduced, preventing oxidation of the sample and…

  • RunBlue™ Blot Membranes

    Expedeon

    Blot Membranes for use in Dual Run & Blot system. Each membrane is sandwiched between two thick pieces of blotting paper for protection which are further separated by blue paper inserts for easy identification of individual blot sandwiches. Pore size - 0.45 µm.

  • 3C-Express

    Expedeon

    3C-Express is a recombinant, restriction grade, HRV3C (Human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly. 3C-Express has robust activity at 4C and high specific activity.…

  • Amintra Glutathione Magnetic Resin for GST-Tagged Proteins

    Expedeon

    GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single purification step. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in…

  • Mitochondrial Protein Isolation Buffer

    Amresco

    Grade: Proteomics A single component mitochondrial protein isolation buffer for enrichment of mitochondrial proteins from cultured mammalian cells. Entire extraction procedure takes less than an hour.

  • PolyMAC

    Expedeon

    Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful phosphoproteomics experiments. The products described here, termed PolyMAC (Polymer-based Metal ion Affinity Capture), overcome the shortcomings of current enrichment methods that typically lack…

  • RunBlue™ Rapid SDS Run Buffer

    Expedeon

    RunBlue RAPID SDS RunBuffer is optimised for use with RunBlue Precast Gels to provide the best running contitions and optimal sample resolution. Based on a Tris-MOPS buffer system this run buffer provides excellent band resolution and fast run time. A 10% RunBlue gel will be run to completion…

  • Amintra Cobalt IDA Resin

    Expedeon

    Amintra CoHIS are affinity resins precharged with Cobalt designed for simple and rapid purification of recombinant HIStagged protein from cell lysate under denaturing or native conditions. Amintra NiHIS and CoHIS resin consists of 45-165 um agarose beads covalently coupled to a chelating group.…

  • Cytoplasmic/Nuclear Protein Enrichment Kit

    Amresco

    Grade: Proteomics The Cytoplasmic/Nuclear Protein Enrichment Kit generates distinct nuclear and cytoplasmic fractions from cultured cells in 1.5 hours. The kit includes 3 buffers that fractionate both suspension cultures and adherent cells with minimal cross-contamination.

  • Amintra Heparin Resin

    Expedeon

    Amintra Heparin Resin is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as antithrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on…

  • FASP™ Protein Digestion Kit

    Expedeon

    Complete Protein Solubilization. Complete Trypsin Digestion. Every Time. Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample…

  • RunBlue™ Run & Blot Systems

    Expedeon

    Expedeon's RunBlue Run & Blot system provides you with a gel tank with unsurpassed functionality. The core module hinges open to accept gel cassettes or buffer dam and then simply clips in place to provide a completely sealed cathode chamber. This allows RunBuffer to be added and samples to…

  • His-Wash Buffer

    Zymo Research Corporation

  • GELFREE® 8100 Cartridge Kits

    Expedeon

    Ready-to-use Gelfree 8100 Cartridge Kits complement the Gelfree 8100 Fractionation Work Station. Each Cartridge Kit contains pre-formulated Running and Sample Buffers, as well as the Fractionation Cartridge. The Gelfree 8100 Fractionation Cartridge contains eight independent channels for molecular…

  • Total Protein Cell Lysis Buffer

    Amresco

    Grade: Proteomics Easy-to-use lysis buffer for extraction of total cell protein. Total Cell Protein Lysis Buffer contains a non-ionic detergent as the lysis agent to reduce denaturation.

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