Isolation & Purification

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Protein Isolation & Purification

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  • pIMAGO-biotin

    Expedeon

    pIMAGO is a universal phosphoprotein detection technology that enables sensitive and specific recognition of phosphorylated molecules. Unlike phospho-antibodies binding is not biased by amino acid sequence, and can therefore be used for detection of any phosphorylation event on any protein site.…

  • RunBlue™ Prestained Markers

    Expedeon

    RunBlue prestained markers enable easy visualization of the marker proteins during and after running and western blotting. The markers can be used to estimate the molecular weight of your protein of interest and assess the transfer efficiency during blotting. Expedeon offers two types of prestained…

  • His-Spin Protein Miniprep Kits

    Zymo Research Corporation

    The His-Spin Protein Miniprep provides researchers with a method for fast His-tagged protein purification. The easy-to-follow procedure is based on a nickel-charged His-Affinity Gel (IMAC), innovative protein purification, and unique Zymo-Spin column technology. Up to 1 mg of His-tagged protein can…

  • Amintra Protein L Resin

    Expedeon

    Protein L is an immunoglobulin-binding protein that was expressed, isolated from the bacterial species Peptostreptococcus magnus.  Protein L Agarose resin offers a unique ability in a convenient way to separate a wider range of mammalian Immunoglobulin classes and sub-classes compare to…

  • YPX™ Yeast Protein Extraction Kit

    Expedeon

    A Simplified Protocol for Higher Yield Extraction Any Morphology. Any Medium. Complete Solubilization. Expedeon's YPX Yeast Protein Extraction Kit is for researchers who need fast, high-yield, unbiased yeast protein extraction and the ability to handle many samples at once. The…

  • TEV-Express

    Expedeon

    TEV-Express contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express does not require any special buffers for its activity and can…

  • RunBlue™ Native Run Buffer

    Expedeon

    RunBlue Native RunBuffer is optimised for use with RunBlue Precast Gels to provide the best running contitions and optimal sample resolution. Based on a Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high moleculr…

  • Yeast Protein Kit

    Zymo Research Corporation

    The Yeast Protein Kit is a simple and convenient method for the rapid, thorough lysis of yeast cells. The kit has been optimized for use with S. cerevisiae and C. albicans but can be used for any fungal species that is susceptible to yeast lytic enzyme (Zymolyase) digestion. The digestion procedure…

  • FastPROTEIN™ Blue Matrix

    MP Biomedicals

    The FastPROTEIN Blue Kit is designed for rapid isolation and purification of protein from bacterial cultures. The FastPROTEIN Blue Matrix, also known as Lysing Matrix B, is used with any FastPrep® Instrument to reproducibly lyse gram positive and gram negative bacterial cells within…

  • RunBlue™ LDS Sample Buffer

    Expedeon

    RunBlue LDS Sample Buffer should be used to prepare all samples for running on RunBlue Precast Gels. Specifically formulated for the RunBlue buffer system to provide the best running conditions and results.

  • FASP™ Protein Digestion Kit

    Expedeon

    Complete Protein Solubilization. Complete Trypsin Digestion. Every Time. Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample…

  • Strep-Spin Protein Miniprep Kits

    Zymo Research Corporation

    The Strep-Spin Protein Miniprep Kit is based on a novel Strep-Tactin XT Superflow resin which binds to Twin-Strep-tag with very high affinity. This tag contains two Strep-tag II peptides connected via a Glycine/Serine linker sequence. Hence this kit is optimized for the purification of…

  • Amintra NHS-Activated Resin

    Expedeon

    Amintra NHS-activated is a highly cross-linked N-hydroxysuccinimide activated matrix. Ligands containing primary amino groups are coupled directly to this active ester to form a chemically stable amide bond.

  • 2D-Xtract™

    G-Biosciences

    A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native charge of the proteins, due to carbamylation.Urea…

  • Amintra Nickel Magnetic Resin for His-Tagged Proteins

    Expedeon

    His-tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing…

  • RunBlue™ Tris-Glycine Blot Buffer

    Expedeon

    RunBlue Tris-Glycine Blotting Buffer is formulated for use with Expedeon Precast SDS-PAGE Gels. Dilution of RunBlue TG Buffer varies between the various blotting aparatuses.

  • Amintra Heparin Resin

    Expedeon

    Amintra Heparin Resin is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as antithrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on…

  • UPX™ Universal Protein Extraction Kit

    Expedeon

    The UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, and an abundance of starting material. One Protein Extraction Protocol for Tissues and Cells, Fresh or Frozen Expedeon's UPX Protein…

  • GELFREE® 8100 Fractionation Station

    Expedeon

    The compact, bench-top Gelfree 8100 Fractionation Station provides specified voltage to each of eight independent channels and pauses for fraction collection according to user programming. Using the touch screen interface, the fractionation sequence is defined or selected, permitting broad mass…

  • Amintra Protein A Magnetic Resin

    Expedeon

    Protein A is immobilised to highly porous and highly cross-linked agarose incorporating magnetic material. The particles have an open pore structure with excellent mass transfer properties for large protein molecules. Amintra Protein A Magnetic can be used in any commercially available magnetic…

  • Amintra Protein G Resin

    Expedeon

    Amintra Protein G resin; a fast flow sepharose matrix covalently modified with recombinant Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with a high capacity and affinity for antibodies. Typically binding capacity of 25 mg…

  • Stabil-PAC

    Expedeon

    Each Stabil-PAC kit contains NVoy technology designed to increase the solubility and stability of proteins whilst preventing aggregation and reducing non-specific binding. Stabil-PAC kits are a convenient way to test if NVoy is able to assist your work. These packs contain single shot amounts of…

  • RunBlue™ Run & Blot Systems

    Expedeon

    Expedeon's RunBlue Run & Blot system provides you with a gel tank with unsurpassed functionality. The core module hinges open to accept gel cassettes or buffer dam and then simply clips in place to provide a completely sealed cathode chamber. This allows RunBuffer to be added and samples to…

  • Amintra Streptavidin Resin

    Expedeon

    Amintra Streptavidin Resin offers an ultra-high biotin-binding capacity with a very low leaching.  Constituted of pure recombinant streptavidin covalently cross-linked and immobilised on fine beaded agarose, Amintra Streptavidin Resin can be used to bind biotinylated biomolecules in a…

  • Amintra Glutathione Resin

    Expedeon

    Amintra's Glutathione resin is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to a high crosslinked agarose matrix that provides a high chemical and physical stability…

  • Amylose Resin

    Zymo Research Corporation

    The Amylose Resin is used for purification of MBP-tagged proteins from cell-free extracts The Amylose Resin is an affinity matrix which specifically binds proteins fused to maltose-binding protein (MBP). When using the Amylose Resin with the MBP-Spin Protein Miniprep Kits, proteins can be…

  • Classical Immunoprecipitation Kit

    G-Biosciences

    Immunoprecipitation (IP) is one of the most useful immunochemical techniques.IPs are routinely used to determinethe presence and quantity of an antigen, molecular weight of a polypeptide, rate of synthesis or degradation, identify certain post translational modifications and interactions with other…

  • Amintra Protein A Resin

    Expedeon

    Amintra Protein A resins are designed on a highly crosslinked sepharose matrix covalently modified with recombinant Protein A and Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with high capacity and affinity for antibodies.

  • RunBlue™ DTT Reducer

    Expedeon

    RunBlue DTT Reducer is a reducing agent which should be added during sample preparation if you wish to perform reduced SDS-PAGE.

  • PolyMAC

    Expedeon

    Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful phosphoproteomics experiments. The products described here, termed PolyMAC (Polymer-based Metal ion Affinity Capture), overcome the shortcomings of current enrichment methods that typically lack…

  • GELFREE® 8100 Cartridge Kits

    Expedeon

    Ready-to-use Gelfree 8100 Cartridge Kits complement the Gelfree 8100 Fractionation Work Station. Each Cartridge Kit contains pre-formulated Running and Sample Buffers, as well as the Fractionation Cartridge. The Gelfree 8100 Fractionation Cartridge contains eight independent channels for molecular…

  • Total Protein Cell Lysis Buffer

    Amresco

    Grade: Proteomics Easy-to-use lysis buffer for extraction of total cell protein. Total Cell Protein Lysis Buffer contains a non-ionic detergent as the lysis agent to reduce denaturation.

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