Isolation & Purification

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Protein Isolation & Purification

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  • RunBlue™ SDS Run Buffer

    Expedeon

    RunBlue SDS Running Buffer is optimised for use with RunBlue Precast Gels to provide the best running conditions and optimal sample resolution. Based on a TEO-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high…

  • Nickel Chelating Resin

    G-Biosciences

    Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni2+) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked…

  • Amintra Protein L Resin

    Expedeon

    Protein L is an immunoglobulin-binding protein that was expressed, isolated from the bacterial species Peptostreptococcus magnus.  Protein L Agarose resin offers a unique ability in a convenient way to separate a wider range of mammalian Immunoglobulin classes and sub-classes compare to…

  • UPX™ Universal Protein Extraction Kit

    Expedeon

    The UPX Universal Protein Extraction Kit is for researchers who need universal, unbiased extraction of both membrane proteins and soluble proteins, and an abundance of starting material. One Protein Extraction Protocol for Tissues and Cells, Fresh or Frozen Expedeon's UPX Protein…

  • FASP™ Protein Digestion Kit

    Expedeon

    Complete Protein Solubilization. Complete Trypsin Digestion. Every Time. Now you can use aggressive, whole proteome solubilization protocols and reagents to prepare proteins from any biological material for trypsin digestion and analysis by mass spectrometry. Based on the Universal Sample…

  • Protein Precipitation Kit

    Amresco

    Removal of interfering salts, detergents, and buffers Reproducible and consistent results This Protein Precipitation Kit utilizes a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and…

  • Pierce™ Premium Grade Sulfo-NHS-LC-Biotin

    Thermo Scientific Pierce

    Thermo Scientific Pierce Premium Grade Sulfo-NHS-LC-Biotin is our highest quality formulation of this popular amine-reactive biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount.

  • RunBlue™ Run & Blot Systems

    Expedeon

    Expedeon's RunBlue Run & Blot system provides you with a gel tank with unsurpassed functionality. The core module hinges open to accept gel cassettes or buffer dam and then simply clips in place to provide a completely sealed cathode chamber. This allows RunBuffer to be added and samples to…

  • pIMAGO-biotin

    Expedeon

    pIMAGO is a universal phosphoprotein detection technology that enables sensitive and specific recognition of phosphorylated molecules. Unlike phospho-antibodies binding is not biased by amino acid sequence, and can therefore be used for detection of any phosphorylation event on any protein site.…

  • GELFREE® 8100 Fractionation Station

    Expedeon

    The compact, bench-top Gelfree 8100 Fractionation Station provides specified voltage to each of eight independent channels and pauses for fraction collection according to user programming. Using the touch screen interface, the fractionation sequence is defined or selected, permitting broad mass…

  • Total Protein Cell Lysis Buffer

    Amresco

    Grade: Proteomics Easy-to-use lysis buffer for extraction of total cell protein. Total Cell Protein Lysis Buffer contains a non-ionic detergent as the lysis agent to reduce denaturation.

  • FastPROTEIN™ Blue Matrix

    MP Biomedicals

    The FastPROTEIN Blue Kit is designed for rapid isolation and purification of protein from bacterial cultures. The FastPROTEIN Blue Matrix, also known as Lysing Matrix B, is used with any FastPrep® Instrument to reproducibly lyse gram positive and gram negative bacterial cells within…

  • FastPROTEIN™ Red Matrix

    MP Biomedicals

    The FastPROTEIN Red Kit is designed for rapid isolation and purification of protein from Yeast cultures. The process is designed to be a small scale test for protein induction. The FastPROTEIN Red Matrix, also known as Lysing Matrix C, is used with the any FastPrep® Instrument to…

  • Mitochondrial Protein Isolation Buffer

    Amresco

    Grade: Proteomics A single component mitochondrial protein isolation buffer for enrichment of mitochondrial proteins from cultured mammalian cells. Entire extraction procedure takes less than an hour.

  • Avidin

    G-Biosciences

    Avidin is a glycoprotein with approximately 10% of its total mass comes from carbohydrates. Avidin has a molecular weight of 67kDa and contains four identical 128 amino acid subunits that each have a single biotin binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and is…

  • ActiveHOOK™ HyperCarrier™

    G-Biosciences

    ActiveHOOK™ HyperCarrier™ is maleimide activated HyperCarrier™, by the addition of sulfoSMCC cross-linker. The ActiveHOOK™ HyperCarrier™ rapidly couples to free sulfhydryl groups on peptides and proteins. This activated carrier proteins save both time and money as…

  • Cytoplasmic/Nuclear Protein Enrichment Kit

    Amresco

    Grade: Proteomics The Cytoplasmic/Nuclear Protein Enrichment Kit generates distinct nuclear and cytoplasmic fractions from cultured cells in 1.5 hours. The kit includes 3 buffers that fractionate both suspension cultures and adherent cells with minimal cross-contamination.

  • Amintra Nickel Magnetic Resin for His-Tagged Proteins

    Expedeon

    His-tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing…

  • Amintra NI-NTA Resin

    Expedeon

    Expedeon's Ni-NTA affinity resin is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Amintra Ni-NTA offers excellent flow properties and high protein binding capacity: in excess of 50mg protein/ml. NTA is a…

  • Amintra Glutathione Resin

    Expedeon

    Amintra's Glutathione resin is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to a high crosslinked agarose matrix that provides a high chemical and physical stability…

  • Amintra Streptavidin Resin

    Expedeon

    Amintra Streptavidin Resin offers an ultra-high biotin-binding capacity with a very low leaching.  Constituted of pure recombinant streptavidin covalently cross-linked and immobilised on fine beaded agarose, Amintra Streptavidin Resin can be used to bind biotinylated biomolecules in a…

  • RunBlue™ Prestained Markers

    Expedeon

    RunBlue prestained markers enable easy visualization of the marker proteins during and after running and western blotting. The markers can be used to estimate the molecular weight of your protein of interest and assess the transfer efficiency during blotting. Expedeon offers two types of prestained…

  • RunBlue™ Native Run Buffer

    Expedeon

    RunBlue Native RunBuffer is optimised for use with RunBlue Precast Gels to provide the best running contitions and optimal sample resolution. Based on a Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high moleculr…

  • Amintra NHS-Activated Resin

    Expedeon

    Amintra NHS-activated is a highly cross-linked N-hydroxysuccinimide activated matrix. Ligands containing primary amino groups are coupled directly to this active ester to form a chemically stable amide bond.

  • RunBlue™ Gel Drying Solution

    Expedeon

  • NVoy Polymers

    Expedeon

    NVoy is a large, uncharged polymer based on a polysaccharide backbone with selected hydrophobic side chains substituted onto the backbone at specific residues to create an amphipathic molecule. NVoy is used in place of detergents, fusion proteins, arginine, chaperones and other common additives…

  • RunBlue™ Antioxidant

    Expedeon

    RunBlue Antioxidant is recommended for use when running reduced samples to provide the best possible resolution of bands. RunBlue Antioxidant runs ahead of the dye front ensuring that the environment through which the protein is migrating is fully reduced, preventing oxidation of the sample and…

  • RunBlue™ Blot Membranes

    Expedeon

    Blot Membranes for use in Dual Run & Blot system. Each membrane is sandwiched between two thick pieces of blotting paper for protection which are further separated by blue paper inserts for easy identification of individual blot sandwiches. Pore size - 0.45 µm.

  • 3C-Express

    Expedeon

    3C-Express is a recombinant, restriction grade, HRV3C (Human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site, and cleaves between Gln and Gly. 3C-Express has robust activity at 4C and high specific activity.…

  • Amintra Glutathione Magnetic Resin for GST-Tagged Proteins

    Expedeon

    GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single purification step. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in…

  • RunBlue™ Rapid SDS Run Buffer

    Expedeon

    RunBlue RAPID SDS RunBuffer is optimised for use with RunBlue Precast Gels to provide the best running contitions and optimal sample resolution. Based on a Tris-MOPS buffer system this run buffer provides excellent band resolution and fast run time. A 10% RunBlue gel will be run to completion…

  • PolyMAC

    Expedeon

    Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful phosphoproteomics experiments. The products described here, termed PolyMAC (Polymer-based Metal ion Affinity Capture), overcome the shortcomings of current enrichment methods that typically lack…

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