Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane. Simply transfer your dilute protein solution to a dialysis bag or dialysis device, such as our patented Tube-O-DIALYZER ™ and then cover the membrane with Concentrator Powder.…

Concentrator Solution is a novel liquid polymer for the rapid concentration of dilute protein solutions with zero loss, using dialysis. Simply transfer your dilute protein solution to a dialysis bag or dialysis device*, such as our patented Tube-O-DIALYZER ™ and dialyze against the…

The UPPA-PROTEIN Concentrate™ kit uses our proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1ng/ml into a small volume. Protein precipitation and concentration are not affected by the presence of detergents,…

Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.…

Tissue-PE LB™ has been developed for extraction of total soluble protein from animal tissues.Tissue- PE LB™ is based on an organic buffer, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of…

Insect Cell-PE LB™ has been developed for extraction of cytoplasmic soluble protein from insect cultured cells. The Insect Cell-PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance…

FOCUS ™ SubCell is designed for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic, and membrane proteins.The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient…

2D electrophoresis and mass spectrometry is routinely used for identification of novel proteins, however the greatest challenge in protein identification is achieving suitable resolution of proteins. The high dynamic range of a species' proteome means that the more abundant proteins mask the…

HOOK™-Sulfo-NHS-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

The Column-PROTEIN-Concentrate™ kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in a low salt buffer…

Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Features Single step extraction protocol. …

Cetyltrimethylammonium bromide extraction solution for genomic DNA extraction. Applications Genomic DNA extraction buffer solution.

Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make…

A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a…

Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released…

PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE.The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents. PAGE-Optimizer™ helps eliminate smeared…

FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent…

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including…

Simple Antibody Biotinylation The HOOK™ IgG Biotinylation (Amine) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (see figure 1). IgG molecules contain a histidine rich region in their Fc region and this feature…

The OrgoSol-PROTEIN-Concentration™ kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains…

Protein samples loaded on iso-electric focusing (IEF) gels should ideally have low conductivity and be free from agents known to interfere with net protein charge. Protein samples containing interfering agents, including ionic detergents, metal ions, salts, lipids, charged polysaccharides,…

Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilizes nearly all of the proteins from plants, including membrane as well as…

Our Universal Protein Precipitation Agent, commonly known as UPPA™, has been specifically designed for the quantitative concentration of protein solutions as dilute as 1ng/ml into a small volume. Protein concentration and precipitation is not affected by the presence of detergents,…

HOOK™-NHS-dPEG™₄-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of…

Bacterial PE LB™ in Phosphate buffer is a variation of Bacterial PE LB™ buffers only in phosphate buffer. It is used preferably for extraction of soluble proteins and soluble recombinant proteins from bacterial cells. Bacterial PE LB™ in Phosphate buffer contains a mild…

FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane proteins are extracted with a single step phase partition, with an efficiency greater than 90%…

Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli , including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife ™ Lysozyme…

FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins. FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A)…

HOOK ™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK ™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB ™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western…

FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20mg phosphorylated ovalbumin per column. The resin…

Specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner…

HOOK™-Sulfo-NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…