Miscellaneous Protein Sample Prep

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G-Biosciences Miscellaneous Protein Sample Prep

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  • The Nuclear & Cytoplasmic Extraction Kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies. This kit is based on…

  • PE LB™ for Yeast

    G-Biosciences

    Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make…

  • FOCUS™ Yeast Proteome

    G-Biosciences

    Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife ™…

  • Mammalian Cell PE LB™ has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to…

  • Simple Antibody Biotinylation The HOOK™ IgG Biotinylation (Amine) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (see figure 1). IgG molecules contain a histidine rich region in their Fc region and this feature…

  • OrgoSol DetergentOUT™

    G-Biosciences

    OrgoSol Detergent OUT ™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. OrgoSol Detergent OUT ™ is compatible with all detergent types and its performance is not dependent on the concentration of detergents in the solution. OrgoSol…

  • PAGE-Optimizer

    G-Biosciences

    PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE.The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents. PAGE-Optimizer™ helps eliminate smeared…

  • HOOK™-Sulfo-NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

  • Concentrator Solution

    G-Biosciences

    Concentrator Solution is a novel liquid polymer for the rapid concentration of dilute protein solutions with zero loss, using dialysis. Simply transfer your dilute protein solution to a dialysis bag or dialysis device*, such as our patented Tube-O-DIALYZER ™ and dialyze against the…

  • Perfect-FOCUS™

    G-Biosciences

    Protein samples loaded on iso-electric focusing (IEF) gels should ideally have low conductivity and be free from agents known to interfere with net protein charge. Protein samples containing interfering agents, including ionic detergents, metal ions, salts, lipids, charged polysaccharides,…

  • FOCUS™ Signal Protein

    G-Biosciences

    Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including…

  • HOOK™-NHS-dPEG™4-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of…

  • FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane proteins are extracted with a single step phase partition, with an efficiency greater than 90%…

  • FOCUS™ PhosphoRich™

    G-Biosciences

    FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20mg phosphorylated ovalbumin per column. The resin…

  • HOOK™-Sulfo-NHS-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

  • The TPE™ kit is a universal lysis system designed for the solubilization and extraction of total proteins from cells and tissues for SDS-PAGE Analysis. The kit is suitable for animal, plant, yeast, bacteria, and other biological samples. The TPE™ kit provides a two component…

  • The SDC™ (Steroid/ Drug/ Compound) Immobilization kit is designed for the immobilization of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit…

  • UPPA™-I & II Pack

    G-Biosciences

    Our Universal Protein Precipitation Agent, commonly known as UPPA™, has been specifically designed for the quantitative concentration of protein solutions as dilute as 1ng/ml into a small volume. Protein concentration and precipitation is not affected by the presence of detergents,…

  • The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic and nuclear proteins, which is fully compatible with 2D gel electrophoresis . The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation…

  • FOCUS™ Insect Proteome

    G-Biosciences

    Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Features Single step extraction protocol. …

  • Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane. Simply transfer your dilute protein solution to a dialysis bag or dialysis device, such as our patented Tube-O-DIALYZER ™ and then cover the membrane with Concentrator Powder.…

  • The OrgoSol-PROTEIN-Concentration™ kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains…

  • FOCUS™ Plant Proteome

    G-Biosciences

    Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilizes nearly all of the proteins from plants, including membrane as well as…

  • Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli , including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife ™ Lysozyme…

  • FOCUS™ Glycoprotein

    G-Biosciences

    FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins. FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A)…

  • A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a…

  • Specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner…

  • HOOK™-Sulfo-NHS-SS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

  • CTAB Extraction Solution

    G-Biosciences

    Cetyltrimethylammonium bromide extraction solution for genomic DNA extraction. Applications Genomic DNA extraction buffer solution.

  • PE LB™ for Insect

    G-Biosciences

    Insect Cell-PE LB™ has been developed for extraction of cytoplasmic soluble protein from insect cultured cells. The Insect Cell-PE LB™ is based on organic buffering agents, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance…

  • Simple Antibody Biotinylation The HOOK™ IgG Biotinylation (Sulfhydryl) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (Figure 1). IgG molecules contain a histidine rich region in their Fc region and this feature…

  • Bacterial PE LB™

    G-Biosciences

    Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released…

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