Media & Reagents

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  • 0.5M EDTA, pH 8.0

    Growcells

    Ethylenediaminetetraacetic acid is a polyamino carboxylic acid, commonly used to deactivate metal dependent enzymes, or as a chelating agent that binds to metal ions. Suitable for RNA storage. Offered in different molar concentration for all common biological applications.

  • Kanamycin Sulfate

    Growcells

    Kanamycin is used as a selective agent by inhibiting protein synthesis in cells that do not have the Kanamycin resistance gene.

  • IPTG (Dioxane Free)

    Growcells

    Isopropyltio-beta-D-thiogalctopyronoside is an inducer of beta-galactosidase activiy in bacteria. Together with chromogenic substates X-Gal or Bluo-Gal, IPTG is used to determine lac gene expression in recombinant methods by binding specifically to the repressor protein of the lac operon. Genomic…

  • PBS (Phosphate Buffered Saline)

    Growcells

    PBST is commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Phosphate Buffered Saline is an optimal formulation of stabilizers, salts and detergents effective in removing excess material from membranes or microtiter plate wells without disrupting…

  • LB Miller's Broth Powder

    Growcells

    Lysogeny broth (LB) -Miller's formula is a nutrionally rich medium optimized for culture of transformed E.coli. To Prepare 1X solution: Add 25g/1000 mL DI water and swirl to create a suspension. Steam sterilize at 15 psi for 20 minutes at 121° C. Allow to cool to 50° C…

  • Ampicillin Sodium Salt (USP Grade)

    Growcells

    A beta-lactam broad spectrum antibiotic used to select ampicillin-resistant plasmids. Ampicillin is a penicillin derivative that inhibits bacterial cell-wall synthesis and is inactivated by lactamases. Recipe: USP Grade Ampicillin Disodium Salt Storage: 4°c Shelf…

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