Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium…

RPMI 1640 was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also…

Calcium and magnesium support cell adhesion. Hanks' Balanced Salts (Modified), without Magnesium and Calcium, is used to minimize cell attachment or aggregation.

Percoll® is a medium for density gradient centrifugation of cells, viruses and subcellular particles. Percoll® is composed of colloidal silica coated with polyvinylpyrrolidone (PVP), which renders the material completely non-toxic and ideal for use with biological materials. Centrifugation…

Calcium and magnesium support cell adhesion. Hanks' Balanced Salt Solution (Modified) is used to minimize cell attachment or aggregation. Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.