ZymoBroth (ZB) is a specially formulated growth medium used for the preparation of highly competent E. coli cells for DNA transformation. When compared to classic SOB growth medium, ZymoBroth dramatically increases transformation efficiency, typically on the order of 5 - 100 fold (depending on the…

Although recombinant protein expression in E. coli has become routine, high level protein expression or overexpression is not always attainable for every protein. Our research has shown that high level protein expression can be achieved consistently when two processes, cell expansion and protein…