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  • 5-Fluoroorotic Acid

    Zymo Research Corporation

    Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…

  • IPTG

    Zymo Research Corporation

    Isopropyl-β-D-thiogalactopyranoside (IPTG).

  • X-GAL

    Zymo Research Corporation

    5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL).

  • YPD Plus™

    Zymo Research Corporation

    The outgrowth step in yeast transformation protocols is often critical for increasing overall yeast transformation efficiencies. Following treatment of yeast with lithium acetate and polyethylene glycol solutions, it is often necessary to add medium to allow for a period of outgrowth, which…

  • 100X 5-Fluoroorotic Acid

    Zymo Research Corporation

    Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…

  • Alpha-Factor Mating Pheromone

    Zymo Research Corporation

    When yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes,…

  • a-Factor Mating Pheromone

    Zymo Research Corporation

    a-factor is one of the two mating pheromones in baking yeast. It is the “opposite” sex of mating pheromone a-Factor (alpha-factor). When yeast a and a cells encounter the opposite mating pheromones, they induce genes necessary for mating, arrest the cell cycle in G1, altering cell surface and…

  • ZymoBroth

    Zymo Research Corporation

    ZymoBroth (ZB) is a specially formulated growth medium used for the preparation of highly competent E. coli cells for DNA transformation. When compared to classic SOB growth medium, ZymoBroth dramatically increases transformation efficiency, typically on the order of 5 - 100 fold (depending on the…

  • Overexpression Broth (OB)

    Zymo Research Corporation

  • Expansion Broth (EB)

    Zymo Research Corporation

  • Dual Media Set

    Zymo Research Corporation

    Although recombinant protein expression in E. coli has become routine, high level protein expression or overexpression is not always attainable for every protein. Our research has shown that high level protein expression can be achieved consistently when two processes, cell expansion and protein…

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