Purification Kits

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  • YPD Plus™

    Zymo Research Corporation

    The outgrowth step in yeast transformation protocols is often critical for increasing overall yeast transformation efficiencies. Following treatment of yeast with lithium acetate and polyethylene glycol solutions, it is often necessary to add medium to allow for a period of outgrowth, which…

  • Alpha-Factor Mating Pheromone

    Zymo Research Corporation

    When yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes,…

  • 100X 5-Fluoroorotic Acid

    Zymo Research Corporation

    Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…

  • 5-Fluoroorotic Acid

    Zymo Research Corporation

    Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted…

  • IPTG

    Zymo Research Corporation

    Isopropyl-β-D-thiogalactopyranoside (IPTG).

  • X-GAL

    Zymo Research Corporation

    5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL).

  • a-Factor Mating Pheromone

    Zymo Research Corporation

    a-factor is one of the two mating pheromones in baking yeast. It is the “opposite” sex of mating pheromone a-Factor (alpha-factor). When yeast a and a cells encounter the opposite mating pheromones, they induce genes necessary for mating, arrest the cell cycle in G1, altering cell surface and…

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