Medium used for the isolation of enteric bacilli based on the fermentation of lactose.

Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121°C (15 lbs. of pressure) for 15 minutes. Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This…

Suspend 24 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Sterilize at 121°C (15 lbs. of pressure) for 15 minutes. Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and…

General purpose growth media to isolate and cultivate yeasts and molds from a wide range of sample sources.

Suspend 30 g of the medium in one liter of distilled or deionized water. Mix well. Heat slowly until the medium is dissolved. Dispense and sterilze at 121°C (15 lbs. of pressure) for 15 minutes. Sterilize by autoclave. Tryptic Soy Broth is used for the cultivation of fastidious and…

(PDA Agar) Suited for the cultivation of yeasts and molds from dairy products and other foods. It may also be used to induce sporulation in many fungi.

Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the…

Sterilize in an autoclave between 118 and 121°C at a pressure of no more than 15 lbs. for 15 minutes. In the case of large volume preparation, increase the time of sterilization but not the temperature or pressure. Cool and pour into Petri dishes. For hemolytic studies, add 5 to 10% sheep or…