Preparation of Liquids from Powder Plant Tissue Culture Media: 1. Measure out approximately 25% less purified water (distilled or deionized) than the final volume of medium required. The water should be at room temperature (15°C to 30°C). 2. Stir the water and slowly add the powder. Rinse…

General purpose growth media to isolate and cultivate yeasts and molds from a wide range of sample sources.

Casein Peptone, Type I. Casein Peptone is supplemented to microbiological growth media.

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I. To be added aseptically to media as a supplement. Prior to use of media, it may be necessary to adjust pH and sterile filter (optional, reduces contamination).

Suspend 30 g of the medium in one liter of distilled or deionized water. Mix well. Heat slowly until the medium is dissolved. Dispense and sterilze at 121°C (15 lbs. of pressure) for 15 minutes. Sterilize by autoclave. Tryptic Soy Broth is used for the cultivation of fastidious and…

Chemicals are carefully selected and, whenever possible, analytical grade materials were used. Finished batches are checked to ensure that they conform to preset high standards before they are released for sale. Except where otherwise indicated, the amounts shown relate to single strength media. …

Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the…

Includes the macro- and mironutrients as described by Murashige and Skoog, 1962, and vitamins as described by Gamborg, et al., 1966.

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.

Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121°C (15 lbs. of pressure) for 15 minutes. Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This…

Molecular Biology Certified™ LB Lennox Agar medium is one of the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in an encapsulated format for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and…

Sterilize in an autoclave between 118 and 121°C at a pressure of no more than 15 lbs. for 15 minutes. In the case of large volume preparation, increase the time of sterilization but not the temperature or pressure. Cool and pour into Petri dishes. For hemolytic studies, add 5 to 10% sheep or…

In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor…

Casamino acids are a mixture of amino acids (except tryptophan) obtained from acid hydrolysis of casein. Applications Casamino acids is supplemented to microbiological growth media. CAS Number 91079-40-2

LB medium, a common bacterial growth medium for E.coli cells, is widely used in molecular biology. Molecular Biology Certified™ Main components are Tryptone and Yeast Extract Nutritionally rich medium Supports growth of E. coli cells Contains essential electrolytes for…

Suspend 24 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Sterilize at 121°C (15 lbs. of pressure) for 15 minutes. Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and…

1× Park and Terasaki's Modification Of McCoy 5A is used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures. It is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and…

100X BME, Modified Vitamins Without L-glutamine Shipped frozen with dry ice

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first…

Hoagland's Modified Basal Salt Mixture includes the macro- and micronutrients as described by Hoagland and Arnon, 1950.

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance Provides cells with water and certain bulk inorganic ions…

Medium used for the isolation of enteric bacilli based on the fermentation of lactose.

Includes the vitamins as described by Murashige and Skoog, 1962. Use at a concentration of 1 ml/L medium.

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated…

For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media. Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and…

Murashige and Skoog Medium contains Murashige and Skoog salts, as well as a mixture of vitamins. This modification of the original formula does not contain agar. Murashige and Skoog Medium is widely used plant tissue culture growth medium.

(PDA Agar) Suited for the cultivation of yeasts and molds from dairy products and other foods. It may also be used to induce sporulation in many fungi.

Ham's F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela…

Circlegrow agar media is used for growth of E.coli and extends plasmid production past log phase. package size 2.3kg

Cellvation Cryopreservation Media is animal component free and has been shown to preserve the potential of stem cell for differentiation. This medium is recommended for the preservation of stem cells, hepatocytes, tissue samples, and a wide variety of cell types and established cell lines. …

Dulbecco’s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding…