Sterilize in an autoclave between 118 and 121°C at a pressure of no more than 15 lbs. for 15 minutes. In the case of large volume preparation, increase the time of sterilization but not the temperature or pressure. Cool and pour into Petri dishes. For hemolytic studies, add 5 to 10% sheep or…

To rehydrate the powdered medium, dissolve 29.5 grams in one liter of water. Dispense as desired. Sterilize in the autoclave for 15 minutes at 15 pounds pressure at 121°C (Catalog number 16821 is a pre-sterilized solution). For more rapid growth of pathogenic bacteria, dissolve 1 gram of agar…

Suspend 30 g of the medium in one liter of distilled or deionized water. Mix well. Heat slowly until the medium is dissolved. Dispense and sterilze at 121°C (15 lbs. of pressure) for 15 minutes. Sterilize by autoclave. Tryptic Soy Broth is used for the cultivation of fastidious and…

Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121°C (15 lbs. of pressure) for 15 minutes. Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This…

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I. To be added aseptically to media as a supplement. Prior to use of media, it may be necessary to adjust pH and sterile filter (optional, reduces contamination).

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.

(PDA Agar) Suited for the cultivation of yeasts and molds from dairy products and other foods. It may also be used to induce sporulation in many fungi.

Dulbecco’s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding…

Preparation Method: Add 5% less distilled water than the desired total volume to a mixing container just large enough to hold the final media volume. Add powdered medium to room temperature (20 -30°C) water with gentle stirring. Do not heat water. Rinse inside of pouch to remove all traces of…

Casamino acids are a mixture of amino acids (except tryptophan) obtained from acid hydrolysis of casein. Applications Casamino acids is supplemented to microbiological growth media. CAS Number 91079-40-2

Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the…

Includes the macro- and mironutrients as described by Murashige and Skoog, 1962, and vitamins as described by Gamborg, et al., 1966.

M9 Minimal Medium Salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids…

Chemicals are carefully selected and, whenever possible, analytical grade materials were used. Finished batches are checked to ensure that they conform to preset high standards before they are released for sale. Except where otherwise indicated, the amounts shown relate to single strength media. …

For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media. Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and…

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. It has proven effective in the culture of tissue derived from…

Molecular Biology Certified™ Synonyms: Luria Broth Base with Agar (Miller modification) L-Broth Agar is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. MP Biomedicals offers L-Broth Agar medium in an encapsulated format…

For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media. Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and…

Quintuple dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Molecular Biology Certified For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media Select for…

Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations. Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of…

Triple dropout formulation of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Also known as Hopkins Mixture and is available in complete and dropout formulations. Preparation Method Reagents are tested and chosen for their ability to promote…

4+ dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations. Recommended Use Add 0.54 g of CSM-Ade-His-Leu-Lys-Trp-Ura to each liter of DOB (or DOBA) and autoclave at 121°C for…

Molecular Biology Certified™ LB Lennox Agar medium is one of the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in an encapsulated format for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and…

Ham's F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela…

MP Biomedicals recommends this medium for the growth and support of cells on microcarrier matrices. High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized…

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance Provides cells with water and certain bulk inorganic ions…

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first…

SC-Ura, also known as Hopkins Mixture, is a single dropout formulation of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the Drop Out Base…

BioRich 1 with 10 common anchorage dependent cell lines commonly show increased yields from 8% to 114% when compared to standard media in normal serum-containing systems. BioRich 1 also shows elevated growth of hybridoma lines at reduced serum concentrations when compared to normal base media.…

CIRCLEGROW® is used for growth of E.coli and extends plasmid production past log phase. Molecular Biology Certified™ Obtain rapid growth of E.coli High yields of plasmid DNA At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second…

Preparation of Liquids from Powder Plant Tissue Culture Media: 1. Measure out approximately 25% less purified water (distilled or deionized) than the final volume of medium required. The water should be at room temperature (15°C to 30°C). 2. Stir the water and slowly add the powder. Rinse…