Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121°C (15 lbs. of pressure) for 15 minutes. Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This…

Circlegrow™ is used for growth of E.coli and extends plasmid production past log phase. Molecular Biology Certified™ Obtain rapid growth of E.coli High yields of plasmid DNA At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second…

Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the…

Preparation Method: Add 5% less distilled water than the desired total volume to a mixing container just large enough to hold the final media volume. Add powdered medium to room temperature (20 -30°C) water with gentle stirring. Do not heat water. Rinse inside of pouch to remove all traces of…

Molecular Biology Certified™ LB Lennox Agar medium is one of the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in an encapsulated format for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and…

Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Chemicals are carefully selected and, whenever possible, analytical grade materials were used. Finished batches are checked to ensure that they conform to preset high standards before they are released for sale. Except where otherwise indicated, the amounts shown relate to single strength media. …

LB medium, a common bacterial growth medium for E.coli cells, is widely used in molecular biology. Molecular Biology Certified™ Main components are Tryptone and Yeast Extract Nutritionally rich medium Supports growth of E. coli cells Contains essential electrolytes for…

4+ dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations. Recommended Use Add 0.54 g of CSM-Ade-His-Leu-Lys-Trp-Ura to each liter of DOB (or DOBA) and autoclave at 121°C for…

(PDA Agar) Suited for the cultivation of yeasts and molds from dairy products and other foods. It may also be used to induce sporulation in many fungi.

Suspend 30 g of the medium in one liter of distilled or deionized water. Mix well. Heat slowly until the medium is dissolved. Dispense and sterilze at 121°C (15 lbs. of pressure) for 15 minutes. Sterilize by autoclave. Tryptic Soy Broth is used for the cultivation of fastidious and…

CIRCLEGROW® is used for growth of E.coli and extends plasmid production past log phase. Molecular Biology Certified™ Obtain rapid growth of E.coli High yields of plasmid DNA At the end of log phase, cells growing in Circlegrow begin to utilize a proprietary second…

Suspend 24 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Sterilize at 121°C (15 lbs. of pressure) for 15 minutes. Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and…

Includes the vitamins as described by Murashige and Skoog, 1962. Use at a concentration of 1 ml/L medium.

BioRich 1 with 10 common anchorage dependent cell lines commonly show increased yields from 8% to 114% when compared to standard media in normal serum-containing systems. BioRich 1 also shows elevated growth of hybridoma lines at reduced serum concentrations when compared to normal base media.…

In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor…

(50X Concentrate) Hypoxanthine 13.61 mg/L Aminopterin 2H₂O 0.1906 mg/L Thymidine 3.876 mg/L Selective for hybridoma cell growth and growth of cells with a functioning HGPRT mechanism. Note: the item below has a Dry Ice Fee included in the price.

Dulbecco’s Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding…

M9 Minimal Medium Salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. The resulting M9 medium can be used for maintaining positive selection pressure on plasmids coding for genes to produce essential substances such as amino acids…

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first…

Quintuple dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Molecular Biology Certified For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media Select for…

Triple dropout formulation of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Also known as Hopkins Mixture and is available in complete and dropout formulations. Preparation Method Reagents are tested and chosen for their ability to promote…

Hoagland's Modified Basal Salt Mixture includes the macro- and micronutrients as described by Hoagland and Arnon, 1950.

1× Park and Terasaki's Modification Of McCoy 5A is used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures. It is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and…

EDTA solution for use as a gentle non-enzymatic cell dissociation reagent.

100X BME, Modified Vitamins Without L-glutamine Shipped frozen with dry ice Note: the item below has a Dry Ice Fee included in the price.

Cellvation Cryopreservation Media is animal component free and has been shown to preserve the potential of stem cell for differentiation. This medium is recommended for the preservation of stem cells, hepatocytes, tissue samples, and a wide variety of cell types and established cell lines. …

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first…

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance Provides cells with water and certain bulk inorganic ions…

For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media. Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and…

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.

Molecular Biology Certified™ Synonyms: Luria Broth Base with Agar (Miller modification) L-Broth Agar is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. MP Biomedicals offers L-Broth Agar medium in an encapsulated format…