HiMedia Tissue Culture Media
HIMEDIA
State-of-the-Art products in microbiology,cell culture, plant tissue culture, and molecular biology.
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HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37deg C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which…
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20X PBS used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt…
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, HEPES buffer, Sodium bicarbonate and Trace elements. Contains stabililzed dipeptide of L-Glutamine.
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Phosphate Buffered Saline, pH 7.2 10X. Commonly used for tissue disaggregation and monolayer dispersal.
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Dulbecco’s Modified Eagle Medium (DMEM) w/ 4.5gms Glucose per litre, L- Alanyl-L-Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate. Contains stabililzed dipeptide of L-Glutamine.
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Nutrient Mixture F-12 Ham, Coon’s Modification w/Sodium bicarbonate w/o L-Glutamine.
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Dulbecco's Phosphate Buffered Saline 1X w/o Phenol red, Calcium and Magnesium. Commonly used for tissue disaggregation and monolayer dispersal.
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Trypan blue 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used for live-dead cell counting.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Yeast Extract
HiMediaYeast Extract is the water-soluble portion of autolyzed yeast. It provides naturally occurring B-complex vitamins, nitrogen, amino acids and carbon in cell culture media. TCL041 is a sterile filtered solution of Yeast Extract. It is most commonly used as an enrichment supplement to grow Mycoplasma.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely…
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Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…
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TCL029 is ZymeFreeTM cell dissociation reagent in Hank's balanced salt solution. It is used for gentle removal of adherent cells from each other and from the surface of a culture vessel. This enzyme free formulation helps in preserving the structural and functional integrity of the cell surface…
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The HiMedia 7.5% potassium bicarbonate solution is mainly used in research facilities and chemical laboratories to conduct wide range of experiments. It is available in sturdy bottle that endures heavy and rigorous usage. Application: Potassium Bicarbonate is used to Supplement Sheilds and Sangs…
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L-Glutamine is an essential amino acid required by all mammalian and insect cells grown in culture. L-glutamine degrades rapidly in solution through an intramolecular cyclization reaction forming ammonia as a byproduct. HiGlutaXL™ supplement contains the stabilized dipeptide form of L-glutamine,…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Dulbecco’s Modified Eagle Medium (DMEM) w/ 4.5gms Glucose per litre, L- Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate. As per ATCC composition.
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Tryptose phosphate broth is used to supplement cell culture media. It is most commonly used to enhance the growth supporting property of Glasgow's Minimum Essential Medium for BHK-21 cells. It is also used in insect cell culture media to support the growth of SF-21 cells.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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HiMedia 1N Sodium Hydroxide
HiMediaTCL002 is 1N Sodium hydroxide solution prepared in cell culture grade water. It is used to buffer cell culture medium as per application.
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TCL019 is tissue culture grade sterile filtered water. It is prepared by passing water through continuous process which includes water pretreatment, reverse osmosis, deionization, and filtration. It is produced specifically for cell culture applications like reconstitution of powdered media and…