HiMedia Tissue Culture Media
HIMEDIA
State-of-the-Art products in microbiology,cell culture, plant tissue culture, and molecular biology.
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Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…
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ZymeFree™ Enzyme Free Cell Dissociation Reagent in Dulbecco’s Phosphate Buffered Saline 1X. Chemically defined cell dissociation reagent used for gentle dissociation of adherent cells.
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ZymeFree™ is cell dissociation reagent in Dulbecco's Phosphate buffered saline. It is used for gentle removal of adherent cells from each other and from the surface of a culture vessel. This enzyme free formulation helps in preserving the structural and functional integrity of the cell surface…
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, HEPES buffer, Sodium bicarbonate and Trace elements. Contains stabililzed dipeptide of L-Glutamine.
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Dulbecco's Phosphate Buffered Saline 1X w/ Calcium and Magnesium w/o Phenol red. Commonly used for tissue disaggregation and monolayer dispersal.
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Water, Sterile, Molecular Biology Grade Endotoxin tested, DEPC treated, Nuclease and Protease free. Used for molecular biology applications like DNA/RNA purification, protein applications and experiments involving RNA.
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Yeast Extract
HiMediaYeast Extract is the water-soluble portion of autolyzed yeast. It provides naturally occurring B-complex vitamins, nitrogen, amino acids and carbon in cell culture media. TCL041 is a sterile filtered solution of Yeast Extract. It is most commonly used as an enrichment supplement to grow Mycoplasma.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Trypan blue 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used for live-dead cell counting.
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Dulbecco’s Modified Eagle Medium (DMEM) w/ 4.5gms Glucose per litre, L- Alanyl-L-Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate. Contains stabililzed dipeptide of L-Glutamine.
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10X PBS used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt…
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Phosphate Buffered Saline, pH 7.2 1X. Commonly used for tissue disaggregation and monolayer dispersal.
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HEPES is a zwitterionic organic buffering agent. It is stable, water soluble and does not react with divalent cations. It has a pKa of 7.5 at 37deg C and offers an optimal buffering range of pH 6.8 - 8.2. It maintains the physiological pH despite changes in the carbon dioxide concentrations which…
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1X PBS is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced…
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Sodium bicarbonate Solution 7.5%
HiMediaSodium bicarbonate Solution 7.5%. Used as a biological buffer in cell culture media to maintain physiological pH.
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The HiMedia water/sterile/tissue culture grade endotoxin tested is mainly used in research facilities and chemical laboratories to conduct wide range of experiments. This DEPC treated compound is nuclease and protease free. It is available in sturdy bottle that endures heavy and rigorous usage.
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Sodium Pyruvate Solution 100mM
HiMediaSodium Pyruvate Solution 100mM. Used as a medium supplement for energy source and a carbon skeleton for anabolic processes.
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Hanks' Balanced Salt Solution 10X w/ Phenol red w/o Sodium bicarbonate. Used with cells maintained in less CO2 environment or CO2 free environment.
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Gelatin Solution 2% w/ 2% Type B Gelatin from bovine skin in sterile tissue culture grade water. Used in cell culture for coating the cell culture plates and flasks to improve the cell attachment for certain types of cells.
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Hanks' Balanced Salt Solution 1X w/ Phenol red and Sodium bicarbonate w/o Calcium. Used with cells maintained in less CO2 environment or CO2 free environment.
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HEPES 1M Solution
HiMediaHEPES 1M Solution. Used in cell culture media to maintain the pH levels of basal media.
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Uridine is a ribonucleoside that has Uracil attached to its ribose ring. It is an important component of carbohydrate metabolism and is essential for both RNA and DNA synthesis. The monophosphate form of uridine (UMP) is formed from orotidylic acid (orotidine 5'- monophosphate) in a decarboxylation…
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Nutrient Mixture F-12 Ham, Coon’s Modification w/Sodium bicarbonate w/o L-Glutamine.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Dulbecco's Phosphate Buffered Saline is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a…
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Tryptose Phosphate Broth 50X
HiMediaTryptose Phosphate Broth 50X. Used to enhance the growth supporting property of GMEM for BHK-21 cells. It is also used in insect cell culture media to support the growth of SF-21 cells.
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Minimum Essential Medium Eagle (MEM) w/ Earle’s salts, L-Glutamine, NEAA and 2.2gms per litre Sodium bicarbonate. As per ATCC composition
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Phenol red 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used as a pH indicator in cell culture media.
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20X PBS used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt…