HiMedia Tissue Culture Media
HIMEDIA
State-of-the-Art products in microbiology,cell culture, plant tissue culture, and molecular biology.
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McCoy's 5A Medium
HiMediaMcCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Water, Sterile, Tissue Culture Grade. Used for reconstitution of powdered media and other cell culture reagents.
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, HEPES buffer, Sodium bicarbonate and Trace elements. Contains stabililzed dipeptide of L-Glutamine.
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer. Contains stabililzed dipeptide of L-Glutamine.
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1X PBS is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced…
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Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…
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ZymeFree™ Enzyme Free Cell Dissociation Reagent in Dulbecco’s Phosphate Buffered Saline 1X. Chemically defined cell dissociation reagent used for gentle dissociation of adherent cells.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Dulbecco's Phosphate Buffered Saline is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various hematological and molecular biology procedures. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a…
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Dulbecco’s Modified Eagle Medium (DMEM) w/ 4.5gms Glucose per litre, L- Alanyl-L-Glutamine, 3.7 gms per litre Sodium bicarbonate and Sodium pyruvate. Contains stabililzed dipeptide of L-Glutamine.
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Sodium bicarbonate Solution 7.5%
HiMediaSodium bicarbonate Solution 7.5%. Used as a biological buffer in cell culture media to maintain physiological pH.
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Technical Data Sheet
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Dulbecco's Phosphate Buffered Saline 1X w/o Phenol red, Calcium and Magnesium. Commonly used for tissue disaggregation and monolayer dispersal.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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ZymeFree™ is cell dissociation reagent in Dulbecco's Phosphate buffered saline. It is used for gentle removal of adherent cells from each other and from the surface of a culture vessel. This enzyme free formulation helps in preserving the structural and functional integrity of the cell surface…
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Yeast Extract
HiMediaYeast Extract is the water-soluble portion of autolyzed yeast. It provides naturally occurring B-complex vitamins, nitrogen, amino acids and carbon in cell culture media. TCL041 is a sterile filtered solution of Yeast Extract. It is most commonly used as an enrichment supplement to grow Mycoplasma.
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CMRL media are based on the composition of Medium 199. Nucleic acid derivatives, coenzymes omitted in H597 modification were retained in CMRL 1066. CMRL 1066 is a chemically defined medium formulated for long term cultivation of mouse L cells in the absence of protein supplement. Although…
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Nutrient Mixture F-12 Ham, Coon’s Modification w/Sodium bicarbonate w/o L-Glutamine.
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Uridine is a ribonucleoside that has Uracil attached to its ribose ring. It is an important component of carbohydrate metabolism and is essential for both RNA and DNA synthesis. The monophosphate form of uridine (UMP) is formed from orotidylic acid (orotidine 5'- monophosphate) in a decarboxylation…
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Tryptose Phosphate Broth 50X
HiMediaTryptose Phosphate Broth 50X. Used to enhance the growth supporting property of GMEM for BHK-21 cells. It is also used in insect cell culture media to support the growth of SF-21 cells.
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The HiMedia water/sterile/tissue culture grade endotoxin tested is mainly used in research facilities and chemical laboratories to conduct wide range of experiments. This DEPC treated compound is nuclease and protease free. It is available in sturdy bottle that endures heavy and rigorous usage.
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Trypan blue 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used for live-dead cell counting.
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Sodium Pyruvate Solution 100mM
HiMediaSodium Pyruvate Solution 100mM. Used as a medium supplement for energy source and a carbon skeleton for anabolic processes.
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Water, Sterile, Molecular Biology Grade Endotoxin tested, DEPC treated, Nuclease and Protease free. Used for molecular biology applications like DNA/RNA purification, protein applications and experiments involving RNA.
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Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…
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HEPES 1M Solution
HiMediaHEPES 1M Solution. Used in cell culture media to maintain the pH levels of basal media.
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Phenol red 0.5% Solution in Dulbecco’s Phosphate Buffered Saline. Used as a pH indicator in cell culture media.
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Phosphate Buffered Saline, pH 7.2 1X. Commonly used for tissue disaggregation and monolayer dispersal.
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