Pump seals should be replaced when there are leaks on the bottom of the pump head, when retention times are inconsistent, or when the pressure ripple is unstable. Each time the seals are replaced, the pistons (plungers) should be examined for scratches. Scratched pistons will cause small…

Produced specifically to proprietary tolerances Reduced lamp-to-lamp variation Increased operating life Lamps for HPLC Detectors are produced using a new manufacturing process that results in more consistent operation characteristics. Lamps feature a more robust, more evenly…

The gold seal should be replaced after the valve has been removed a few times.

Common to all 1100/1200 pump modules, the purge valve has a gold seal held in place with a plastic cup The most frequently replaced item in the 1100/1200 Series pumps is the purge valve PTFE frit, which can clog with seal material or solvent particulates When replacing the frit,…

Agilent's certified lamps are manufactured under ISO 9001 certified environment and are fully traceable through out every step of the production process. Each lamp is then tested to ensure it meets Agilent's specifications for performance. Test equipment is regularly calibrated using…

Sample loops for Rheodyne 7725 Series and 7125 Series valves are not interchangeable due to the change in port angle Actual volumes can differ due to tolerance of metal tubing bore Accuracy of large metal loops is ±5%, intermediate loops ±10%, small loops ±30%

Unfiltered solvents or solvents contaminated with microbial growth can clog solvent inlet filters, reducing the lifetime of the filter and impacting pump performance. Remember to clean or replace the solvent inlet filters at least once every 3 months.

Flexible PEEK loops have a clean, straight cut for low dead volume connections Inert to most organic solvents Wall thickness, temperature, exposure time and concentration of organic solvents affect the durability of PEEK tubing Concentrated nitric acid and sulfuric acid weaken PEEK…

Maximum sensitivity in the low UV wavelength range High injection precision High retention time reproducibility Flow rates below 0.5 ml/min

Different columns have different fitting requirements. (Zorbax columns use standard Swagelok fittings) Agilent 1100 modules use standard Swagelok fittings Rheodyne injection valves require Rheodyne fittings How do you choose the correct fittings? To ensure leak-free connections, and to prevent…

Rotor seal replacement is the most common injection valve maintenance procedure.

The needle should be replaced when it becomes bent, burred or blunt. The needle seat should be replaced when it is leaking or plugged. You should suspect a leak if you notice a trail of buffer crystals on the needle seat. The needle seat can become blocked if the sample contains particulates, as…

Stainless steel Most popular injection valve for analytical HPLC Loops range from 1 µl to 5 ml (20 µl loop installed) Built in position sensing switch provides the LC with reproducible start signal Selected as Agilent 1050 and 1100 manual injectors

A set of valve types specially designed for the Agilent 1100/1200 Series HPLC system allows you to extend your HPLC applications. More flexibility in solvent selection and column selection New automation capabilities in sample preparation Increased sample throughput through…

Rotor seal replacement is the most common injection valve maintenance procedure.

Large sample volumes, high flow rates and preparative columns 100 µl to 20 ml (10 ml loop installed) For biomolecules, the 3725i has PEEK materials

Before exchanging the outlet ball valve, try cleaning it in a sonicator by placing the valve in an upright position (onto the plastic cap) in a small beaker with alcohol. Sonicate for 5 to 10 minutes. The 1100/1200 Series binary pump has a different outlet ball valve which includes a sieve.

The routine use of highly concentrated buffer solutions (100 mM) will reduce the life of the seals and pistons in your 1100/1200 Series pump. You can counteract the problem with one of our seal wash kits, which flushes the backside of the seal with a wash solvent; water/isopropanol…

Rotor seals wear with use and need routine replacement Stators only need replacement if the ports are damaged PEEK rotor seals are incompatible with concentrated nitric and sulfuric acids

PEEK flow path is perfect for biomolecules?sample does not contact metal Similar features as 7725i, including the “make-before-break” design

These are the recommended replacement multipliers and horns for the MSD. Other manufacturers’ products may be incompatible with Agilent instruments and can result in reduced sensitivity, lifetime, and noise problems. The electron multiplier replacement horn (2713M88) is for use with 5975,…