IEF performed under denaturing conditions gives the highest resolution and the cleanest results. Urea solubilizes and unfolds most proteins to their fully random conformation, with all ionizable groups exposed to solution. Urea is included in the 2-D sample solution at a concentration of at least 8 M.

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength. Highly suitable for DNA/RNA applications. Highly suitable for DNA/RNA applications.Agarose NA…

PlusOne Acrylamide PAGE contains extremely low concentrations of impurities that can interfere with the polymerization reaction. It is especially suitable for SDS and native PAGE techniques. PlusOne Acrylamide PAGE contains extremely low concentrations of impurities that can interfere with the…

HMW Native Marker Kit Determine molecular weight of uncharacterized protein samples with precision by comparison with carefully selected standards. Get clear, sharp bands for easy measurement of migration distances. Bands can be visualized by using Deep Purple Total Protein Stain, Coomassie…

Pharmalyte carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. Each interval contains numerous ampholytes with a high buffering capacity per pH unit. Pharmalyte carrier…

Glycine is frequently used as a component of electrophoresis buffers, especially in the Laemmli discontinuous buffer system for SDS-PAGE.

RunBlue Bis-Tris 10x10cm Protein Gels have been developed by Expedeon to provide an alternative to NuPAGE® Bis-Tris gels. RunBlue Bis-Tris Protein gels run with the same voltages and times, in the same tanks, using the same MES/MOPS buffers as NuPAGE® Bis-Tris gels and achieve highly…

PlusOne Bind-Silane PlusOne Bind-Silane Bind-Silane (?-methacryloxypropyltrimethoxysilane) is used to covalently attach polyacrylamide gels to a glass surface. The gel stays firmly attached to the glass during staining and drying procedures.

PlusOne N,N'-Methylene-bisacrylamide PlusOne N,N'-Methylene-bisacrylamide PlusOne N,N'-Methylenebisacrylamide is a general cross-linker used with acrylamide for PAGE techniques. It is available as a dry powder and a premixed solution.

Glycerol is a stabilizing medium for casting polyacrylamide gradients. In IEF, the pH gradient can be stabilized by introducing 10?15% glycerol. It also stabilizes polyacrylamide gels for preservation.

PlusOne Bromophenol Blue is an electrophoresis tracking dye used in IEF, PAGE and Sequencing. PlusOne Bromophenol Blue is an electrophoresis tracking dye used in IEF, PAGE and Sequencing.

PlusOne TEMED PlusOne TEMED TEMED is a catalyst for the polymerization of acrylamide when used with ammonium persulfate.

Broad pI Kit Broad pI Kit pl markers are lyophilized mixtures of stable, salt-free, highly purified proteins. The markers may be run in parallel with the experimental samples on isoelectric focusing gels. These markers are not prestained. The precisely defined pI of the markers…

Qualified for 2-D electrophoresis, Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of 7.2 to 9.0. Qualified for 2-D electrophoresis, Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of…

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength. Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications. Suitable…

A nonionic or zwitterionic detergent is included in the sample solution to ensure complete sample solubilization and to prevent aggregation through hydrophobic interactions. CHAPS and Triton X-100 are detergents commonly used in IEF

DNA/RNA techniques Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications. If separated nucleic acids are to be used as substrates for enzymes (e.g., ligase and restriction endonucleases), a special highly purified agarose such as Agarose NA should be used. For…

Amersham QuickStain enables protein detection directly after SDS-PAGE or transfer. Supports Western blot normalization for quantitative Western blotting. Quick SDS-PAGE analysis: Prelabel protein samples with ready-to-use Cy5 dye for quick, sensitive detection without gel staining and…

RunBlue Native Protein Gels are pre-cast high resolution gels for PAGE analysis of native proteins and protein complexes. A more neutral environment and optimised buffer minimises protein modification leading to sharper, more reliable results. RunBlue Native Protein Gels have a range of usability…

RunBlue Native Mini Protein Gels are precast high resolution gels for PAGE analysis of native proteins and protein complexes. A more neutral environment and optimised buffer minimises protein modification leading to sharper, more reliable results. RunBlue Native Protein Gels have a range of…

RunBlue SDS Protein Gels are precast high resolution gels for SDS-PAGE. Their novel chemistry system and unique manufacturing process ensure very sharp bands and reproducible results. They have a range of usability enhancements such as being tear proof and comb free, and are compatible with most…

RunBlue precast gels have been developed as an improvement on the current state of the art precast SDS-PAGE gels. RunBlue gels are more rigid and up to 10 times stronger than conventional precast gels resulting in more robust gels that can be handled with confidence without tearing. 100% Compatible…