Pharmalyte carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. Each interval contains numerous ampholytes with a high buffering capacity per pH unit. Pharmalyte carrier…

Qualified for 2-D electrophoresis, Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of 7.2 to 9.0. Qualified for 2-D electrophoresis, Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of…

Amersham QuickStain enables protein detection directly after SDS-PAGE or transfer. Supports Western blot normalization for quantitative Western blotting. Quick SDS-PAGE analysis: Prelabel protein samples with ready-to-use Cy5 dye for quick, sensitive detection without gel staining and…

DNA/RNA techniques Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications. If separated nucleic acids are to be used as substrates for enzymes (e.g., ligase and restriction endonucleases), a special highly purified agarose such as Agarose NA should be used. For…

PlusOne Repel-Silane ES PlusOne Repel-Silane ES Repel-Silane ES is a 2% solution of dimethyldichlorosilane dissolved in octamethylcyclooctasilane, a low toxicity, environmentally safe solvent. It is used to inhibit sticking of polyacrylamide gels, agarose gels, and nucleic acids to glass surfaces.

Glycine is frequently used as a component of electrophoresis buffers, especially in the Laemmli discontinuous buffer system for SDS-PAGE.

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength. Highly suitable for DNA/RNA applications. Highly suitable for DNA/RNA applications.Agarose NA…

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength. Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications. Suitable…

Broad pI Kit Broad pI Kit pl markers are lyophilized mixtures of stable, salt-free, highly purified proteins. The markers may be run in parallel with the experimental samples on isoelectric focusing gels. These markers are not prestained. The precisely defined pI of the markers…

HMW Native Marker Kit Determine molecular weight of uncharacterized protein samples with precision by comparison with carefully selected standards. Get clear, sharp bands for easy measurement of migration distances. Bands can be visualized by using Deep Purple Total Protein Stain, Coomassie…

IEF performed under denaturing conditions gives the highest resolution and the cleanest results. Urea solubilizes and unfolds most proteins to their fully random conformation, with all ionizable groups exposed to solution. Urea is included in the 2-D sample solution at a concentration of at least 8 M.