IEF performed under denaturing conditions gives the highest resolution and the cleanest results. Urea solubilizes and unfolds most proteins to their fully random conformation, with all ionizable groups exposed to solution. Urea is included in the 2-D sample solution at a concentration of at least 8 M.

A nonionic or zwitterionic detergent is included in the sample solution to ensure complete sample solubilization and to prevent aggregation through hydrophobic interactions. CHAPS and Triton X-100 are detergents commonly used in IEF

Amersham QuickStain enables protein detection directly after SDS-PAGE or transfer. Supports Western blot normalization for quantitative Western blotting. Quick SDS-PAGE analysis: Prelabel protein samples with ready-to-use Cy5 dye for quick, sensitive detection without gel staining and…

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength. Highly suitable for DNA/RNA applications. Highly suitable for DNA/RNA applications.Agarose NA…

Pharmalyte carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. Each interval contains numerous ampholytes with a high buffering capacity per pH unit. Pharmalyte carrier…

HMW Native Marker Kit Determine molecular weight of uncharacterized protein samples with precision by comparison with carefully selected standards. Get clear, sharp bands for easy measurement of migration distances. Bands can be visualized by using Deep Purple Total Protein Stain, Coomassie…

Broad pI Kit Broad pI Kit pl markers are lyophilized mixtures of stable, salt-free, highly purified proteins. The markers may be run in parallel with the experimental samples on isoelectric focusing gels. These markers are not prestained. The precisely defined pI of the markers…

PlusOne Acrylamide PAGE contains extremely low concentrations of impurities that can interfere with the polymerization reaction. It is especially suitable for SDS and native PAGE techniques. PlusOne Acrylamide PAGE contains extremely low concentrations of impurities that can interfere with the…

PlusOne TEMED PlusOne TEMED TEMED is a catalyst for the polymerization of acrylamide when used with ammonium persulfate.

Qualified for 2-D electrophoresis, Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of 7.2 to 9.0. Qualified for 2-D electrophoresis, Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of…

DNA/RNA techniques Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications. If separated nucleic acids are to be used as substrates for enzymes (e.g., ligase and restriction endonucleases), a special highly purified agarose such as Agarose NA should be used. For…

Glycine is frequently used as a component of electrophoresis buffers, especially in the Laemmli discontinuous buffer system for SDS-PAGE.

PlusOne Bind-Silane PlusOne Bind-Silane Bind-Silane (?-methacryloxypropyltrimethoxysilane) is used to covalently attach polyacrylamide gels to a glass surface. The gel stays firmly attached to the glass during staining and drying procedures.

PlusOne N,N'-Methylene-bisacrylamide PlusOne N,N'-Methylene-bisacrylamide PlusOne N,N'-Methylenebisacrylamide is a general cross-linker used with acrylamide for PAGE techniques. It is available as a dry powder and a premixed solution.

Glycerol is a stabilizing medium for casting polyacrylamide gradients. In IEF, the pH gradient can be stabilized by introducing 10?15% glycerol. It also stabilizes polyacrylamide gels for preservation.

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength. Agarose with low electroendosmosis (EEO) is recommended for most DNA/RNA applications. Suitable…

PlusOne Bromophenol Blue is an electrophoresis tracking dye used in IEF, PAGE and Sequencing. PlusOne Bromophenol Blue is an electrophoresis tracking dye used in IEF, PAGE and Sequencing.