The 5-methylcytosine (5-mC) modification of DNA in epigenetic regulation has been well studied over the last several decades. However, the role of the so-called ôsixth baseö, 5-hydroxymethylcytosine (5-hmC), has yet to be defined. Recent studies have associated 5-hmC patterns to…

ZymoTaq DNA Polymerase is a hot-start polymerase that is ideal for amplification of bisulfite-converted DNA. Because it is a heat-activated, thermostable DNA polymerase, ZymoTaq reduces primer dimer and nonspecific product formation, whereas conventional polymerases typically exhibit these problems…

The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research; To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated…

The Pico Methyl-Seq Library Prep Kit provides a streamlined workflow for making WGBS libraries. Input DNA is randomly fragmented during the initial bisulfite treatment step followed by three rounds of amplification with uniquely designed primers. The procedure can accommodate as…

The EZ-96 DNA Methylation-Lightning MagPrep Kit features rapid and reliable bisulfite treatment and conversion of DNA coupled to a magnetic bead based clean-up for high-throughput methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is…

The Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator provides a hassle-free method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse cross-linking, Proteinase K or RNase A…

DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme…

The EZ-96 DNA Methylation MagPrep features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. Desulphonation and…

Micrococcal Nuclease cleaves single stranded and double stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono and oligonucleotides with 3' phosphates.

The Methylated  &  Non-methylated pUC19 DNA Set consists of two control DNAs (methylated and non-methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, and EZ DNA Methylation-Direct kits from…

The Femto Bacterial DNA Quantification Kit can detect and quantify bacterial DNA with high specificity and sensitivity. Bacterial DNA can be reliably quantified in a background of non-bacterial DNA, such as human, fungal, animal DNA, etc. This is essential for downstream applications that require…

The Femto Human DNA Quantification Kit can detect and quantify human DNA with high specificity and sensitivity. Human DNA can be reliably quantified in a background of non-human DNA, such as bacterial, fungal, animal, plant DNA, etc. This is essential for downstream applications that require…

The Universal Methylated Mouse DNA Standard is designed for use as control to assess the efficiency of bisulfite-mediated conversion of DNA in combination with the EZ DNA Methylation, EZ DNA Methylation- Gold, or EZ DNA Methylation-Direct kits.<br>The control DNA has been enzymatically…

The 5-methylcytosine (5-mC) modification of DNA in epigenetic regulation has been well studied over the last several decades. However, the role of the so-called ôsixth baseö, 5-hydroxymethylcytosine (5-hmC), has yet to be defined. Recent studies have associated 5-hmC patterns to…

The Human Methylated & Non-methylated DNA Set consists of two control DNAs (a methylated human DNA standard and a non-methylated human DNA standard) together with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, and EZ…

The EZ-96 DNA Methylation-Direct MagPrep features simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or…

Mouse Kidney DNA (5 ug).

Methylated/Non-methylated Control DNA & Primer Set.

,p>Mouse Liver DNA (5 ug).

20X SAM (S-adenosylmethionine) (200 ul).

dsDNA Shearase Plus (50 Units)

dsDNA Shearase Plus 50 U (1 U/ul) + DCC-5 (D4013).

Negative Control.

Human WGA Non-Methylated DNA (5 ug / 20 ul)

Mouse Brain DNA (5 ug).

E. coli Non-methylated Genomic DNA.

Femto Human qPCR Premix.

The RRHP 5-hmC Library Prep Kit is the first comprehensive solution for quantitative analysis of genome-wide 5-hmC positions at single base resolution. The Reduced Representation Hydroxymethylcytosine Profiling(RRHP) system is based on blocking MspI digestion by glucosylating 5-hmC…

The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research; To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated…

The Human Methylated & Non-methylated DNA Set consists of two control DNAs (a methylated human DNA standard and a non-methylated human DNA standard) together with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation, EZ DNA Methylation-Gold, and EZ…