Tight-binding, reversible, highly selective inhibitor of acid proteases (aspartyl peptidases), like pepsin, gastricsin, cathepsin E and D, renin, chymosin, bacterial aspartic proteinases and HIV proteases. Does not inhibit thiol proteases, neutral proteases or serine proteases. Widely used as a…

Collagenases degrade native helical collagen fibrils. Plays an important role in connective tissue metabolism Produced by specific cells involved in repairs and remodeling processes Type II enzyme that contains greater clostripain activity Collagenase is used for collagen…

Pectinase is a macerating enzyme comprised of pectintranseliminase, polygalacturonase, pectinesterase, xylanase, hemicellulases and cellulases. Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase causes random hydrolysis of…

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6 phosphate and D-fructose 6-phosphate. Responsible for the second step of glycolysis Involved in glucogenesis Highly conserved in bacteria and eukaryotes Phosphoglucose Isomerase fuctions as an…

Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus. Extracts group specific antigens …

HRP is a plant glycohemoprotein that belongs to the ferroprotoporphyrin group of peroxidases. HRP is composed of seven isozymes. All isozymes contain photohemin IX as prosthetic group. Neutral and amino sugars account for approximately 18% of the enzyme. The iron-containing hemin group is…

Collagenases degrade native helical collagen fibrils. It has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. Collagenase is used for tissue dissociation combined with other enzymes such as elastase, trypsin,…

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Pepsin, an acid protease, contains a proteolytic enzyme. Pepsin contains the "cathepsin" component which has milk curdling activity. It has a broad range of substrate activity and demonstrates an esterase acitivity. It generally attacks peptide bonds. Pepsin is a peptidase used to…

Fungus-Derived Proteinase K Purified Recombinant is prepared at 20 mg/mL in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This Molecular Biology Grade Proteinase K solution is extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases…

Protease and Phosphatase Inhibitor Cocktail contains a mixture of inhibitors and is designed to prevent protein degradation by endogenous proteases and phosphatases released during the extraction of proteins from animal cells or tissues. Application Notes: Dilute 1/10 in RIPA or Lysis Buffer…

MAO inhibitor; inhibits platelet aggregation.

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis. Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of…

Phosphorylation and dephosphorylation of proteins controls the processes of cell differentiation, proliferation, signal transduction, and apoptosis. Covalent binding of phosphate groups can change the conformation of proteins and the binding capacity of ligands. During protein extraction from…

β-Mercaptoethylamine is a useful antioxidant .It is experimentally used as a radioprotective agent and It is also used to produce acute and chronic duodenal ulcers in rats and as an antidote to acetaminophen. β-Mercaptoethylamine derived from cysteine degradation is the simplest stable…

Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Enzyme…

Potent platinum-based antineoplastic agent with wide spectrum anticancer activity. Anticancer compound. Forms inter- and intrastrand DNA adducts/crosslinks, consequently blocking DNA replication and transcription and inducing cell death. Shows antitumor activity in cisplatin resistant cell lines.…

Papain is a sulfhydryl protease from Carica papaya latex. (A second protease, chymopapain, and a lysozyme have also been isolated from this same source.) Since native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide, it may exist as a…

Glucose oxidase is an FAD-containing glycoprotein. The enzyme is specific for β-D-glucose. O can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each…

PMA activates Ca2+- ATPase and potentiates forskolin-induced cAMP formation. It has been shown to inhibit apoptosis induced by the Fas antigen, but PMA induces apoptosis in HL-60 promyelocytic leukemia cells.

Cellulase is used for protoplast preparations. This enzyme shows a very high filter paper decomposing activity, as well as, appreciable xylanase and hemicellulase activity. It is an effective enzyme for use with pectolyase Y-23 for plant cell wall removal. It has been used in a study to assess the…

Biliverdin Dihydrochloride is a bile pigment component. An oxidized metabolite of bilirubin caused when bilirubin reacts with reactive oxygen species. Biliverdin IX is composed of alpha, beta, gamma and delta isomers. Biliverdin dihydrochloride was found responsible for cytoprotection. It was…

β-Galactosidase is useful for the structural investigation of carbohydrate, the determination of lactose (foodstuff analysis) and as an enzyme label for enzyme immunoassay. β-Galactosidase cleaves terminal galactose residues, which are β -1-->14 linked to a monosaccharide,…

Cellulase refers to a group of enzymes which, acting together, hydrolyze cellulose. The b-glucosidases, including cellobiose, are primarily active on the smaller molecular weight cellulose hydrolysates. During cellulose breakdown they are active on the dimers and oligomers of cellulose. …

Aldehyde dehydrogenase belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. Aldehyde dehydrogenase oxidizes a number of aliphatic and aromatic aldehydes. Acetyl-GSH is not hydrolyzed. Aldehyde…

2,2'-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic Acid) Solution is oxidized to a radical action showing absorption maxima at 820 nm, 734 nm, 650 nm and 405 nm, in the presence of hydrogen peroxide and HRP. The latter frequency demonstrates a significant molar extinction coefficient and is…

Collagenase products are prepared from culture supernatants of C. histolyticum. It is prepared to contain lower tryptic activity to limit damage to membrane proteins and receptors but with normal to above normal collagenase activity. Suggested for pacreatic islet isolation. Crude Collagenases…

Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand and a chelating agent. Ethylenediamine Tetraacetic Acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity. Zinc-dependent…

Hyaluronidase is a glycoprotein containing 5% mannose and 2.17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- β-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a…

α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. α-Amylase is…

Papain is a sulfhydryl protease from Carica papaya latex. Cleaves peptide bonds of basic amino acids, leucine, or glycine Hydrolyzes esters and amides One unit will hydrolyze 1.0 µmole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C and pH 6.2 Papain…

Anticancer compound. Chemotherapeutic used in patients with cancer and advanced forms of Kaposi's sarcoma. Microtubule assembly stabilizer. Reversibly binds to polymerized tubulin. Anti-mitotic. Mitotic spindle assembly, chromosome segregation and cell division inhibitor. Induces cell cycle…