Electrophoresis Reagents

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Electrophoresis Reagents

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Attention Thomas Scientific Customers

Due to current pandemic, lead times for PPE items are longer than what is listed on our website. Please expect shipping delays of all PPE products during this time.

  • Seeband Protein Stain is a convenient alternative to traditional coomassie blue staining procedures. Environmentally friendly, this ready-to-use stain does not contain methanol or acetic acid, and does not require hazardous solvents for destaining. This simple “hands-off ” staining/destaining…

  • Blue-Clean Protein Stain

    IBI Scientific

    Coomassie Blue Staining of proteins in SDS-PAGE gels is a daily procedure in many laboratories. It is very popular among life science researchers due to its good sensitivity and relative ease of use. Traditionally, coomassie blue staining requires a methanol and acetic acid solution to achieve…

  • 6X Loading Dye

    IBI Scientific

    IBI’s ready-to-use 6X loading dye is ideally suited for agarose electrophoresis of DNA, RNA, or nucleic acids. Its ultra pure grade ensures quality and repeatibility every time you use it. The light blue, indigo, and magenta dyes will clearly show the migration of each run, and the dyes…

  • For new users, we recommend GelGreen® 10,000X in water, our latest formulation that eliminates the hazards of handling DMSO for better safety. Replaces SYBR or GelStar. Excites at 488nm.

  • Glycerol

    IBI Scientific

    Glycerol is used to prevent freezing during enzyme and bacterial preparations.

  • The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

  • Ethidium Bromide

    IBI Scientific

    Ethidium Bromide can be used to detect both single and double stranded nucleic acids (both DNA and RNA). However, Ethidium Bromide does not intercollate into single-stranded DNA very well; therefore, the fluorescence of single-stranded DNA is relatively poor in comparison to double-stranded DNA.…

  • GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed® is far more sensitive than EB without requiring a…

  • HRP-conjugated secondary antibodies for immunoblotting applications Advansta’s high-quality HRP-conjugated secondary antibodies offer unparalleled sensitivity and performance for immunoblotting and ELISA applications. These secondary HRP conjugated antibodies are optimized with…

  • AccuBlue® High Sensitivity is a non-mutagenic fluorescent dsDNA quantitation assay for fluorescence microplate reader. The kit offers sensitive and selective detection of purified dsDNA samples with excellent linearity.

  • IPTG

    IBI Scientific

    IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications, thus allowing detection of recombinant molecules.

  • Acridine Orange

    Biotium

    Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm).The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.

  • 2X Protein Loading Dye

    IBI Scientific

    IBI’s 2X Protein Loading Dye helps track the migration progression of your sample during polyacrylamide electrophoresis. The loading dye not only gives color to the sample, but also increases the density to ensure efficient distribution in each well. IBI’s loading dye migrates independently of the…

  • PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive DNA-binding dye that is useful for determination of microbial viability by qPCR.

  • Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

  • DAPI

    Biotium

    DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.

  • GelGreen® is a sensitive, stable and environmentally safe green fluorescent nucleic acid dye designed to stain either dsDNA, ssDNA or RNA in agarose gels. GelGreen® is far more sensitive than SYBR Safe. We continue to offer GelGreen® 10,000X solution in DMSO for established users who…

  • These ready to use solutions make it fast and easy to prepare polyacrylamide electrophoresis gels. IBI also offers acrylamide and bisacrylamide in individual solution form, which allows the user to mix bisacrylamide solutions in multiple ratios, giving the user greater flexibility while only having…

  • The AccuBlue® Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution provides a linear detection range from 2 – 2000 ng and is compatible with fluorescence microplate readers.…

  • AdvanStain Ponceau rapidly detects proteins on nitrocellulose and PVDF membranes, allowing you to check the quality of protein transfer before proceeding to Western blotting. With AdvanStain Ponceau, you can quickly make sure that protein transfer has been even across the entire blot, and that no…

  • X-Gal

    IBI Scientific

    X-GAL is histochemical substrate for β-galactosidase, allowing the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20mg/ml solution. Note: Use either a glass or polypropylene tube. Add 4ml of this solution per liter of…

  • GelRed® Nucleic Acid Stain, 10,000X

    Phenix Research Products

    Phenix offers GelRed® 10,000X in water and GelRed® 10,000X in DMSO. GelRed® in water is a newer, safer formulation and recommended format. We continue to offer GelRed® in DMSO for established users who do not wish to alter their protocols. Replaces EtBr. Excites at 302/312nm.

  • EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis.

  • Formamide, Spectral Grade

    IBI Scientific

    Formamide is used as a denaturant in hybridization and electrophoresis applications. Formamide is used to denature DNA and RNA. It separates the two strands of the double helix.

  • Seeband Protein Stain is a convenient alternative to traditional coomassie blue staining procedures. Environmentally friendly, this ready-to-use stain does not contain methanol or acetic acid, and does not require hazardous solvents for destaining. This simple “hands-off ” staining/destaining…

  • The AccuBlue® High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution is intended for use on a fluorecent plate reader in a 96-well microplate format.

  • 5-Aminoallyl-dUTP (AA-dUTP) in lyophilized form suitable for long-term storage. 5-Aminoallyl-dUTP can be enzymatically incorporated into DNA.

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