EICOSANOIDS-EIA (Enzyme Immunoassay)

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Cayman Chemical EICOSANOIDS-EIA (Enzyme Immunoassay)

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  • Leukotriene B4 ELISA Kit

    Cayman Chemical

    LTB4 is synthesized from arachidonic acid by the combined action of 5-LO and LTA4 hydrolase. LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release,…

  • Lipid Hydroperoxide (LPO) Assay Kit

    Cayman Chemical

    Cayman’s Lipid Hydroperoxide Assay measures the hydroperoxides directly utilizing the redox reactions with ferrous ions. An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure…

  • COX-2 (human) Inhibitor Screening Assay Kit

    Cayman Chemical

    COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-2 (human recombinant) Inhibitor Screening Assay measures PGF2α that is produced by SnCl2 reduction of COX-2-derived…

  • sPLA2 (human Type IIA) ELISA Kit

    Cayman Chemical

    Cayman’s PLA2 ELISA Kit is an immunometric (i.e. "sandwich") assay that can be used for the quantification of sPLA2 in plasma, synovial fluid, and other sample matrices. Each kit contains AChE:s PLA2 antibody conjugate, antiserum, human recombinant sPLA2 standard, Ellman’s…

  • sPLA2 (Type V) Inhibitor Screening Assay Kit

    Cayman Chemical

    The sPLA2 (Type V) Inhibitor Screening Assay is a convenient colorimetric assay designed for rapid screening of Type V sPLA2 inhibitors in a 96-well format. Each kit contains assay buffer, DTNB, diheptanoyl thio-PC, human sPLA2 (Type V), a 96-well plate, plate cover, and complete instructions.

  • Prostaglandin D2 ELISA Kit

    Cayman Chemical

    Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a…

  • 8-Isoprostane ELISA Kit

    Cayman Chemical

    The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also…

  • Triglyceride Colorimetric Assay Kit

    Cayman Chemical

    The measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell…

  • Fatty Acid Amide Hydrolase Inhibitor Screening Assay Kit

    Cayman Chemical

    FAAH is a cytosolic serine hydrolase responsible for the degradation of fatty acid amides, including AEA. Finding inhibitors to FAAH could offer a beneficial approach toward the treatment of pain, obesity, and various neurological diseases where higher endocannabinoid activity would be beneficial.…

  • Prostaglandin E2 ELISA Kit - Monoclonal

    Cayman Chemical

    Cayman’s PGE2 ELISA is a sensitive competitive assay that uses a high-affinity PGE2 monoclonal antibody for quantification of PGE2 in plasma, urine, and culture media samples.

  • Free Fatty Acid Fluorometric Assay Kit

    Cayman Chemical

    The measurement of FFA can be useful in determining metabolic status. Cayman’s Free Fatty Acid (FFA) Assay can be used for measuring free fatty acids in plasma, serum, and urine. The FFA Assay utilizes a coupled enzymatic reaction that results in generation of the highly fluorescent product resorufin.

  • Thromboxane B2 ELISA Kit

    Cayman Chemical

    For the quantification of TXB2 in urine, serum, tissue culture supernatants, and other sample matrices Sensitivity (80% B/B0): 5 pg/ml Assay Range: 1.6-1,000 pg/ml Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet…

  • COX (ovine) Colorimetric Inhibitor Screening Assay Kit

    Cayman Chemical

    The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm. Inhibition of COX activity by a variety of selective and nonselective inhibitors shows…

  • COX Activity Assay Kit

    Cayman Chemical

    The COX Activity Assay utilizes the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm.The assay can be used to measure COX activity (COX-1 and COX-2) in cell lysates, tissue homogenates, and purified…

  • Soluble Epoxide Hydrolase Cell-Based Assay Kit

    Cayman Chemical

    Mammalian sEH is a member of the α/β-hydrolase fold family of enzymes that catalyze the hydrolysis of exogenous and endogenous epoxides to vicinal diols. Endogenous substrates for sEH include EETs which exhibit vasodilatory and anti-inflammatory activity. Cayman’s sEH Cell-Based…

  • COX-2 (ovine) Inhibitor Screening Assay Kit

    Cayman Chemical

    COX-1 and COX-2 catalyze the production of the prostaglandin (PG), thromboxane, and prostacyclin precursor PGH2. COX-2 is responsible for the biosynthesis of PGs under acute inflammatory conditions. This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory…

  • Latanoprost ELISA Kit

    Cayman Chemical

    Latanoprost is an F-series PG analog which has been approved for use as an ocular hypotensive drug. PG esters and other similar derivatives act as prodrugs which are converted to the active free acid form by an esterase/amidase activity in ocular tissues. The free acid form of Latanoprost is 200…

  • Resolvin D2 ELISA Kit

    Cayman Chemical

    Resolvin D2 (RvD2) is produced physiologically from the sequential oxygenation of docosahexaenoic acid (DHA) by 15- and 5-lipoxygenases. RvD2 reduces polymorphonuclear leukocyte transendothelial migration, the earliest event in acute inflammation, by ~70% at doses as low as 10 pg in the mouse model…

  • Sphingomyelin Colorimetric Assay Kit

    Cayman Chemical

    Sphingomyelin is an important lipid component of cell membranes and lipoproteins. Cayman’s SM Assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum in a 96-well format with a colorimetric readout at 595 nm.

  • Lipid Hydroperoxide (LPO) Assay Kit

    Cayman Chemical

    Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders. Lipid peroxidation results in the formation of highly reactive, unstable hydroperoxides of both saturated and unsaturated lipids. Cayman’s Lipid Hydroperoxide Assay…

  • STAT-8-Isoprostane ELISA Kit

    Cayman Chemical

    Cayman’s STAT-8-isoprostane ELISA is a competitive assay that permits the rapid measurement of 8-iso PGF2α from biological samples, requiring only 1 hour incubation and development times for each step. This assay format is similar to that employed in Cayman’s original…

  • Prostaglandin Screening ELISA Kit

    Cayman Chemical

    This assay was developed for screening applications in which the relative amount of PG production for a large number of cell culture samples must be determined. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given…

  • 8-Isoprostane Affinity Purification Kit

    Cayman Chemical

    This kit contains all reagents necessary for simple one-step purification of 8-isoprostane from most biological samples.

  • Lipoxygenase Inhibitor Screening Assay Kit

    Cayman Chemical

    This assay kit provides an accurate and convenient method for screening LO inhibitors. This assay measures the hydroperoxides generated from the incubation of a LO (5-, 12-, or 15-LO) with either arachidonic or linoleic acid.

  • COX Colorimetric Inhibitor Screening Assay Kit

    Cayman Chemical

    The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. Inhibition of COX activity, measured…

  • Leukotriene C4 ELISA Kit

    Cayman Chemical

    Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The…

  • Resolvin D1 ELISA Kit

    Cayman Chemical

    Resolvin D1 (RvD1) is produced physiologically from the sequential oxygenation of DHA by 15- and 5-lipoxygenases. The 17(R)-epimer of RvD1 can also be generated with aspirin-treatment. Both RvD1 and its 17(R) configuration reduce human polymorphonuclear leukocyte (PMNL) transendothelial migration,…

  • 11ß-Prostaglandin F2a ELISA Kit

    Cayman Chemical

    11β-PGF2α is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excretion of 11β-PGF2α is about 11 ng/mmol creatinine (~400…

  • Prostaglandin D2-MOX Express ELISA Kit

    Cayman Chemical

    Cayman’s PGD2-MOX Express ELISA is a competitive assay that permits the rapid measurement of PGD2 from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in the PGD2-MOX ELISA (Item No. 512011).…

  • Prostaglandin F2a ELISA Kit

    Cayman Chemical

    PGF2α is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. The majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. Like all of the primary PGs, PGF2α has a very short half-life in the general circulation.…

  • cPLA2 Assay Kit

    Cayman Chemical

    Arachidonoyl thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2 releases free thiol which can be detected by DTNB. This assay can be used to…

  • Prostaglandin E Metabolite ELISA Kit

    Cayman Chemical

    Because of the rapid metabolism of PGE2, the determination of in vivo PGE2 biosynthesis is often best accomplished by the measurement of PGE2 metabolites. Cayman’s PGEM Assay converts all 13,14-dihydro-15-keto PGE2 and 13,14-dihydro-15-keto PGA2 into a single stable derivative, which is…

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