EICOSANOIDS-EIA (Enzyme Immunoassay)

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EICOSANOIDS-EIA (Enzyme Immunoassay)

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  • Prostaglandin Screening ELISA Kit

    Cayman Chemical

    This assay was developed for screening applications in which the relative amount of PG production for a large number of cell culture samples must be determined. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given…

  • Triglyceride Colorimetric Assay Kit

    Cayman Chemical

    The measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell…

  • Lipoxygenase Inhibitor Screening Assay Kit

    Cayman Chemical

    This assay kit provides an accurate and convenient method for screening LO inhibitors. This assay measures the hydroperoxides generated from the incubation of a LO (5-, 12-, or 15-LO) with either arachidonic or linoleic acid.

  • 11-dehydro Thromboxane B2 ELISA Kit - Monoclonal

    Cayman Chemical

    Thromboxane A2 (TXA2), produced from arachidonic acid, causes irreversible platelet aggregation, vascular, and bronchial smooth muscle contraction. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2. However, most of the TXB2 measured in the plasma or urine of healthy individuals is due to…

  • 15(S)-HETE ELISA Kit

    Cayman Chemical

    15(S)-HETE is produced from arachidonic acid by the enzyme 15-LO. In humans it is formed primarily in the respiratory epithelium, leukocytes, and reticulocytes. It may contribute to allergic rhinitis, has anti-inflammatory properties, and acts as a vasoconstrictor. Cayman’s 15(S)-HETE ELISA…

  • COX Colorimetric Inhibitor Screening Assay Kit

    Cayman Chemical

    The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. Inhibition of COX activity, measured…

  • Free Fatty Acid Fluorometric Assay Kit

    Cayman Chemical

    The measurement of FFA can be useful in determining metabolic status. Cayman’s Free Fatty Acid (FFA) Assay can be used for measuring free fatty acids in plasma, serum, and urine. The FFA Assay utilizes a coupled enzymatic reaction that results in generation of the highly fluorescent product resorufin.

  • Prostaglandin D2-MOX Express ELISA Kit

    Cayman Chemical

    Cayman’s PGD2-MOX Express ELISA is a competitive assay that permits the rapid measurement of PGD2 from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in the PGD2-MOX ELISA (Item No. 512011).…

  • FABP4 Inhibitor/Ligand Screening Assay Kit

    Cayman Chemical

    Cayman’s FABP4 Ligand Binding Assay provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a detection reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will…

  • Prostaglandin D2 Express ELISA Kit

    Cayman Chemical

    Prostaglandin D2 (PGD2) is biosynthesized in the brain by soluble, glutathione-independent lipocalin-type PGD2 synthase. PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular,…

  • Leukotriene E4 ELISA Kit

    Cayman Chemical

    Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously…

  • Prostaglandin E2

    MP Biomedicals

    Prostaglandin E2 is one of the primary cyclooxygenase products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. The effects of PGE2 are transduced by at…

  • Biotrak™ Prostaglandin E2 Enzymeimmunoassay (EIA) System

    GE Healthcare

    The Prostaglandin E2 (PGE2) Competitive Biotrak™ Enzymeimmunoassay System from GE Healthcare is specifically designed for research purposes. RPN222 includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of PGE2 from cell cultures and plasma samples.…

  • Latanoprost ELISA Kit

    Cayman Chemical

    Latanoprost is an F-series PG analog which has been approved for use as an ocular hypotensive drug. PG esters and other similar derivatives act as prodrugs which are converted to the active free acid form by an esterase/amidase activity in ocular tissues. The free acid form of Latanoprost is 200…

  • Resolvin D2 ELISA Kit

    Cayman Chemical

    Resolvin D2 (RvD2) is produced physiologically from the sequential oxygenation of docosahexaenoic acid (DHA) by 15- and 5-lipoxygenases. RvD2 reduces polymorphonuclear leukocyte transendothelial migration, the earliest event in acute inflammation, by ~70% at doses as low as 10 pg in the mouse model…

  • 11ß-Prostaglandin F2a ELISA Kit

    Cayman Chemical

    11β-PGF2α is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excretion of 11β-PGF2α is about 11 ng/mmol creatinine (~400…

  • Prostaglandin F2a ELISA Kit

    Cayman Chemical

    PGF2α is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. The majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. Like all of the primary PGs, PGF2α has a very short half-life in the general circulation.…

  • Prostaglandin D2 ELISA Kit

    Cayman Chemical

    Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a…

  • Fluprostenol ELISA Kit

    Cayman Chemical

    Fluprostenol is a metabolically stable analog of PGF2α with potent FP receptor agonist activity. The isopropyl ester of fluprostenol has been approved for use as an ocular hypotensive drug for the treatment of glaucoma and is sold under the Alcon trade name Travoprost. Acting as a prodrug,…

  • 6-keto Prostaglandin F1a ELISA Kit

    Cayman Chemical

    Prostacyclin is formed from arachidonic acid primarily by the vascular endothelium and renal cortex. It is a potent vasodilator and inhibitor of platelet aggregation. PGI2 is non-enzymatically hydrated to 6-keto PGF1α (t½ = 2-3 minutes), and then quickly converted to the major…

  • Prostaglandin E2 ELISA Kit - Monoclonal

    Cayman Chemical

    Cayman’s PGE2 ELISA is a sensitive competitive assay that uses a high-affinity PGE2 monoclonal antibody for quantification of PGE2 in plasma, urine, and culture media samples.

  • 17-phenyl trinor Prostaglandin F2a ELISA Kit

    Cayman Chemical

    17-phenyl trinor PGF2α is a metabolically stable analog of PGF2α and is a potent agonist for the FP receptor. The ethyl amide analog has been approved for use as an ocular hypotensive drug, sold under the Allergan trade name Bimatoprost. There is some evidence that unmetabolized…

  • 8-Isoprostane Express ELISA Kit

    Cayman Chemical

    This assay offers the convenience of a fast assay (2 hour incubation; 1 hour development) while still achieving a detection limit (80% B/B0) of 10 pg/ml.

  • Prostaglandin E2 Express ELISA Kit

    Cayman Chemical

    Cayman’s PGE2 Express ELISA is a competitive assay that permits the rapid measurement of PGE2 from biological samples, requiring only 1 hour incubation and development times for each step. The foundation of the assay is Cayman’s high-affinity, well-characterized PGE2 monoclonal, the…

  • 13,14-dihydro-15-keto Prostaglandin F2a ELISA Kit

    Cayman Chemical

    PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α. The Cayman’s…

  • Resolvin D1 ELISA Kit

    Cayman Chemical

    Resolvin D1 (RvD1) is produced physiologically from the sequential oxygenation of DHA by 15- and 5-lipoxygenases. The 17(R)-epimer of RvD1 can also be generated with aspirin-treatment. Both RvD1 and its 17(R) configuration reduce human polymorphonuclear leukocyte (PMNL) transendothelial migration,…

  • COX-1 (ovine) Inhibitor Screening Assay Kit

    Cayman Chemical

    COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-1 (ovine) Inhibitor Screening Assay measures PGF2α that is produced by SnCl2 reduction of COX-1-derived PGH2.…

  • Lipid Hydroperoxide (LPO) Assay Kit

    Cayman Chemical

    Cayman’s Lipid Hydroperoxide Assay measures the hydroperoxides directly utilizing the redox reactions with ferrous ions. An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure…

  • AMP+ Mass Spectrometry Kit

    Cayman Chemical

    Cayman’s AMP+ Mass Spectrometry Kit contains a positively charged reagent (AMP+) that can be coupled quantitatively to free carboxylic acids through an amide bond linkage. The resulting derivatized product thus contains a positively charged group that shows improved electrospray ionization…

  • COX Fluorescent Inhibitor Screening Assay Kit

    Cayman Chemical

    Cayman’s COX Fluorescent Inhibitor Screening Assay provides a convenient fluorescence-based method for screening ovine COX-1 and human recombinant COX-2 for isozyme-specific inhibitors. The assay utilizes the peroxidase component of COXs. In this assay, the reaction between PGG2 and ADHP…

  • tetranor-PGDM ELISA Kit

    Cayman Chemical

    tetranor-PGDM is a major metabolite of PGD2 found in human and murine urine. Normal levels of tetranor-PGDM in human and murine urine are 1.5 ng/mg creatinine and 8.1 ng/mg creatinine respectively. Cayman’s tetranor-PGDM ELISA is a competitive assay that can be used for quantification of…

  • Thromboxane B2 ELISA Kit

    Cayman Chemical

    For the quantification of TXB2 in urine, serum, tissue culture supernatants, and other sample matrices Sensitivity (80% B/B0): 5 pg/ml Assay Range: 1.6-1,000 pg/ml Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet…

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