EICOSANOIDS-EIA (Enzyme Immunoassay)

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EICOSANOIDS-EIA (Enzyme Immunoassay)

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  • COX-1 (human) Inhibitor Screening Assay Kit

    Cayman Chemical

    Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) is a bifunctional enzyme that converts arachidonic acid to a hydroperoxy endoperoxide (PGG2) and reduces the endoperoxide to the corresponding alcohol (PGH2), the precursor of prostaglandins, thromboxanes, and…

  • Prostaglandin D2-MOX Express ELISA Kit

    Cayman Chemical

    Cayman’s PGD2-MOX Express ELISA is a competitive assay that permits the rapid measurement of PGD2 from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in the PGD2-MOX ELISA (Item No. 512011).…

  • Lipid Hydroperoxide (LPO) Assay Kit

    Cayman Chemical

    Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders. Lipid peroxidation results in the formation of highly reactive, unstable hydroperoxides of both saturated and unsaturated lipids. Cayman’s Lipid Hydroperoxide Assay…

  • COX (human) Inhibitor Screening Assay Kit

    Cayman Chemical

    The COX (human) Inhibitor Screening Assay Kit directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using a broadly specific antiserum that binds to all the major prostaglandin compounds. This assay includes…

  • Prostaglandin Screening ELISA Kit

    Cayman Chemical

    This assay was developed for screening applications in which the relative amount of PG production for a large number of cell culture samples must be determined. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given…

  • cPLA2 Assay Kit

    Cayman Chemical

    Arachidonoyl thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2 releases free thiol which can be detected by DTNB. This assay can be used to…

  • COX-1 (ovine) Inhibitor Screening Assay Kit

    Cayman Chemical

    COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-1 (ovine) Inhibitor Screening Assay measures PGF2α that is produced by SnCl2 reduction of COX-1-derived PGH2.…

  • Prostaglandin D2 ELISA Kit

    Cayman Chemical

    Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a…

  • Leukotriene C4 ELISA Kit

    Cayman Chemical

    Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The…

  • Latanoprost ELISA Kit

    Cayman Chemical

    Latanoprost is an F-series PG analog which has been approved for use as an ocular hypotensive drug. PG esters and other similar derivatives act as prodrugs which are converted to the active free acid form by an esterase/amidase activity in ocular tissues. The free acid form of Latanoprost is 200…

  • Biotrak™ Prostaglandin E2 Enzymeimmunoassay (EIA) System

    GE Healthcare

    The Prostaglandin E2 (PGE2) Competitive Biotrak™ Enzymeimmunoassay System from GE Healthcare is specifically designed for research purposes. RPN222 includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of PGE2 from cell cultures and plasma samples.…

  • Free Fatty Acid Fluorometric Assay Kit

    Cayman Chemical

    The measurement of FFA can be useful in determining metabolic status. Cayman’s Free Fatty Acid (FFA) Assay can be used for measuring free fatty acids in plasma, serum, and urine. The FFA Assay utilizes a coupled enzymatic reaction that results in generation of the highly fluorescent product resorufin.

  • Fatty Acid Amide Hydrolase Inhibitor Screening Assay Kit

    Cayman Chemical

    FAAH is a cytosolic serine hydrolase responsible for the degradation of fatty acid amides, including AEA. Finding inhibitors to FAAH could offer a beneficial approach toward the treatment of pain, obesity, and various neurological diseases where higher endocannabinoid activity would be beneficial.…

  • sPLA2 (human Type IIA) ELISA Kit

    Cayman Chemical

    Cayman’s PLA2 ELISA Kit is an immunometric (i.e. "sandwich") assay that can be used for the quantification of sPLA2 in plasma, synovial fluid, and other sample matrices. Each kit contains AChE:s PLA2 antibody conjugate, antiserum, human recombinant sPLA2 standard, Ellman’s…

  • 15(S)-HETE ELISA Kit

    Cayman Chemical

    15(S)-HETE is produced from arachidonic acid by the enzyme 15-LO. In humans it is formed primarily in the respiratory epithelium, leukocytes, and reticulocytes. It may contribute to allergic rhinitis, has anti-inflammatory properties, and acts as a vasoconstrictor. Cayman’s 15(S)-HETE ELISA…

  • Lipid Hydroperoxide (LPO) Assay Kit

    Cayman Chemical

    Cayman’s Lipid Hydroperoxide Assay measures the hydroperoxides directly utilizing the redox reactions with ferrous ions. An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure…

  • 6-keto Prostaglandin F1a ELISA Kit

    Cayman Chemical

    Prostacyclin is formed from arachidonic acid primarily by the vascular endothelium and renal cortex. It is a potent vasodilator and inhibitor of platelet aggregation. PGI2 is non-enzymatically hydrated to 6-keto PGF1α (t½ = 2-3 minutes), and then quickly converted to the major…

  • COX (ovine) Colorimetric Inhibitor Screening Assay Kit

    Cayman Chemical

    The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm. Inhibition of COX activity by a variety of selective and nonselective inhibitors shows…

  • COX Activity Assay Kit

    Cayman Chemical

    The COX Activity Assay utilizes the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized TMPD at 590 nm.The assay can be used to measure COX activity (COX-1 and COX-2) in cell lysates, tissue homogenates, and purified…

  • 11-dehydro Thromboxane B2 ELISA Kit - Monoclonal

    Cayman Chemical

    Thromboxane A2 (TXA2), produced from arachidonic acid, causes irreversible platelet aggregation, vascular, and bronchial smooth muscle contraction. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2. However, most of the TXB2 measured in the plasma or urine of healthy individuals is due to…

  • Prostaglandin E2

    MP Biomedicals

    Prostaglandin E2 is one of the primary cyclooxygenase products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. The effects of PGE2 are transduced by at…

  • Triglyceride Colorimetric Assay Kit

    Cayman Chemical

    The measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell…

  • Fluprostenol ELISA Kit

    Cayman Chemical

    Fluprostenol is a metabolically stable analog of PGF2α with potent FP receptor agonist activity. The isopropyl ester of fluprostenol has been approved for use as an ocular hypotensive drug for the treatment of glaucoma and is sold under the Alcon trade name Travoprost. Acting as a prodrug,…

  • Leukotriene B4 ELISA Kit

    Cayman Chemical

    LTB4 is synthesized from arachidonic acid by the combined action of 5-LO and LTA4 hydrolase. LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release,…

  • STAT-8-Isoprostane ELISA Kit

    Cayman Chemical

    Cayman’s STAT-8-isoprostane ELISA is a competitive assay that permits the rapid measurement of 8-iso PGF2α from biological samples, requiring only 1 hour incubation and development times for each step. This assay format is similar to that employed in Cayman’s original…

  • Prostaglandin F2a ELISA Kit

    Cayman Chemical

    PGF2α is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. The majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. Like all of the primary PGs, PGF2α has a very short half-life in the general circulation.…

  • 8-Isoprostane Express ELISA Kit

    Cayman Chemical

    This assay offers the convenience of a fast assay (2 hour incubation; 1 hour development) while still achieving a detection limit (80% B/B0) of 10 pg/ml.

  • Prostaglandin E2 Express ELISA Kit

    Cayman Chemical

    Cayman’s PGE2 Express ELISA is a competitive assay that permits the rapid measurement of PGE2 from biological samples, requiring only 1 hour incubation and development times for each step. The foundation of the assay is Cayman’s high-affinity, well-characterized PGE2 monoclonal, the…

  • 13,14-dihydro-15-keto Prostaglandin F2a ELISA Kit

    Cayman Chemical

    PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α. The Cayman’s…

  • COX Colorimetric Inhibitor Screening Assay Kit

    Cayman Chemical

    The COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. Inhibition of COX activity, measured…

  • FABP4 Inhibitor/Ligand Screening Assay Kit

    Cayman Chemical

    Cayman’s FABP4 Ligand Binding Assay provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a detection reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will…

  • COX (ovine) Inhibitor Screening Assay Kit

    Cayman Chemical

    The COX (ovine) Inhibitor Screening Assay directly measures PGF2α produced in the cyclooxygenase reaction. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, this Cayman COX Assay is more accurate and…

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