This PGD2-MOX ELISA is based on the conversion of PGD2 to a stable MOX derivative. Treatment of the sample with MOX HCl converts PGD2 into PGD2-MOX, preventing its further chemical degradation. The antiserum used in the assay was developed using conjugates of this derivative and is very specific…

The sPLA2 (Type V) Inhibitor Screening Assay is a convenient colorimetric assay designed for rapid screening of Type V sPLA2 inhibitors in a 96-well format. Each kit contains assay buffer, DTNB, diheptanoyl thio-PC, human sPLA2 (Type V), a 96-well plate, plate cover, and complete instructions.

Sphingomyelin is an important lipid component of cell membranes and lipoproteins. Cayman’s SM Assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum in a 96-well format with a colorimetric readout at 595 nm.

For the quantification of TXB₂ in urine, serum, tissue culture supernatants, and other sample matrices Sensitivity (80% B/B0): 5 pg/ml Assay Range: 1.6-1,000 pg/ml Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet…

PGF2α is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. The majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. Like all of the primary PGs, PGF2α has a very short half-life in the general circulation.…

Thromboxane A2 (TXA2), produced from arachidonic acid, causes irreversible platelet aggregation, vascular, and bronchial smooth muscle contraction. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2. However, most of the TXB2 measured in the plasma or urine of healthy individuals is due to…

The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and…

Prostacyclin is formed from arachidonic acid primarily by the vascular endothelium and renal cortex. It is a potent vasodilator and inhibitor of platelet aggregation. PGI2 is non-enzymatically hydrated to 6-keto PGF1α (t½ = 2-3 minutes), and then quickly converted to the major…

Cayman’s FABP4 Ligand Binding Assay provides a simple, reproducible, and sensitive tool for the identification of FABP4 ligands. The assay makes use of a detection reagent that exhibits increased fluorescence at 500 nm when bound to FABP4. Any strong ligand and/or inhibitor of FABP4 will…

This assay offers the convenience of a fast assay (2 hour incubation; 1 hour development) while still achieving a detection limit (80% B/B0) of 10 pg/ml.

Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid via the 5-lipoxygenase (5-LO) pathway in leukocytes. LTC4, LTD4, and LTE4 are collectively referred to as cysteinyl leukotrienes (CysLTs). LTC4 and LTD4 are potent mediators of asthma and hypersensitivity.…

Cayman’s sPLA2 Assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s with the exception of cPLA2. Upon hydrolysis of the thioester bond at the sn-2…

Cayman’s COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay utilizes the peroxidase component of COXs. In this assay, the reaction…

LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman Chemical’s LDL Uptake Cell-Based Assay employs a preparation of human LDL conjugated to Dylight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A…

COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-1 (ovine) Inhibitor Screening Assay measures PGF2α that is produced by SnCl2 reduction of COX-1-derived PGH2.…

PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α. The Cayman’s…