EICOSANOIDS-EIA (Enzyme Immunoassay)

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EICOSANOIDS-EIA (Enzyme Immunoassay)

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  • Cayman’s Lipid Hydroperoxide Assay measures the hydroperoxides directly utilizing the redox reactions with ferrous ions. An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure…

  • Leukotriene E4 ELISA Kit

    Cayman Chemical

    Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously…

  • This kit contains all reagents necessary for simple one-step purification of 8-isoprostane from most biological samples.

  • Resolvin D2 ELISA Kit

    Cayman Chemical

    Resolvin D2 (RvD2) is produced physiologically from the sequential oxygenation of docosahexaenoic acid (DHA) by 15- and 5-lipoxygenases. RvD2 reduces polymorphonuclear leukocyte transendothelial migration, the earliest event in acute inflammation, by ~70% at doses as low as 10 pg in the mouse model…

  • Prostaglandin F2a ELISA Kit

    Cayman Chemical

    PGF2α is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. The majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. Like all of the primary PGs, PGF2α has a very short half-life in the general circulation.…

  • tetranor-PGDM ELISA Kit

    Cayman Chemical

    tetranor-PGDM is a major metabolite of PGD2 found in human and murine urine. Normal levels of tetranor-PGDM in human and murine urine are 1.5 ng/mg creatinine and 8.1 ng/mg creatinine respectively. Cayman’s tetranor-PGDM ELISA is a competitive assay that can be used for quantification of…

  • Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders. Lipid peroxidation results in the formation of highly reactive, unstable hydroperoxides of both saturated and unsaturated lipids. Cayman’s Lipid Hydroperoxide Assay…

  • Prostacyclin is formed from arachidonic acid primarily by the vascular endothelium and renal cortex. It is a potent vasodilator and inhibitor of platelet aggregation. PGI2 is non-enzymatically hydrated to 6-keto PGF1α (t½ = 2-3 minutes), and then quickly converted to the major…

  • Latanoprost ELISA Kit

    Cayman Chemical

    Latanoprost is an F-series PG analog which has been approved for use as an ocular hypotensive drug. PG esters and other similar derivatives act as prodrugs which are converted to the active free acid form by an esterase/amidase activity in ocular tissues. The free acid form of Latanoprost is 200…

  • COX-1 and COX-2 catalyze the production of the prostaglandin (PG), thromboxane, and prostacyclin precursor PGH2. COX-2 is responsible for the biosynthesis of PGs under acute inflammatory conditions. This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory…

  • Cayman’s PGD2-MOX Express ELISA is a competitive assay that permits the rapid measurement of PGD2 from biological samples, requiring only 1 hour incubation and development times for each step. The methodology for the assay is the same as that employed in the PGD2-MOX ELISA (Item No. 512011).…

  • cPLA2 Assay Kit

    Cayman Chemical

    Arachidonoyl thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2 releases free thiol which can be detected by DTNB. This assay can be used to…

  • PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α. The Cayman’s…

  • Prostaglandin D2 (PGD2) is biosynthesized in the brain by soluble, glutathione-independent lipocalin-type PGD2 synthase. PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a cellular,…

  • This assay was developed for screening applications in which the relative amount of PG production for a large number of cell culture samples must be determined. The antiserum used in this assay exhibits high cross reactivity for most PGs which will allow quantification of all the PGs in a given…

  • 15(S)-HETE ELISA Kit

    Cayman Chemical

    15(S)-HETE is produced from arachidonic acid by the enzyme 15-LO. In humans it is formed primarily in the respiratory epithelium, leukocytes, and reticulocytes. It may contribute to allergic rhinitis, has anti-inflammatory properties, and acts as a vasoconstrictor. Cayman’s 15(S)-HETE ELISA…

  • Cayman’s STAT-8-isoprostane ELISA is a competitive assay that permits the rapid measurement of 8-iso PGF2α from biological samples, requiring only 1 hour incubation and development times for each step. This assay format is similar to that employed in Cayman’s original…

  • COX-1 and COX-2 catalyze the production of the PG and TX precursor PGH2. COX-2 is primarily responsible for the biosynthesis of PGs under acute inflammatory conditions. The COX-1 (ovine) Inhibitor Screening Assay measures PGF2α that is produced by SnCl2 reduction of COX-1-derived PGH2.…

  • The COX (human) Inhibitor Screening Assay Kit directly measures PGF2α by SnCl2 reduction of COX-derived PGH2 produced in the COX reaction. The prostanoid product is quantified via ELISA using a broadly specific antiserum that binds to all the major prostaglandin compounds. This assay includes…

  • Sphingomyelin is an important lipid component of cell membranes and lipoproteins. Cayman’s SM Assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum in a 96-well format with a colorimetric readout at 595 nm.

  • The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics…

  • Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid via the 5-lipoxygenase (5-LO) pathway in leukocytes. LTC4, LTD4, and LTE4 are collectively referred to as cysteinyl leukotrienes (CysLTs). LTC4 and LTD4 are potent mediators of asthma and hypersensitivity.…

  • This assay kit provides an accurate and convenient method for screening LO inhibitors. This assay measures the hydroperoxides generated from the incubation of a LO (5-, 12-, or 15-LO) with either arachidonic or linoleic acid.

  • The COX (ovine/human) Inhibitor Screening Assay directly measures PGF2α produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via ELISA using a broadly specific antibody that binds to all the major PG compounds. Thus, the Cayman COX Assay is more accurate and…

  • sPLA2 Assay Kit

    Cayman Chemical

    Cayman’s sPLA2 Assay provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s with the exception of cPLA2. Upon hydrolysis of the thioester bond at the sn-2…

  • Leukotriene C4 ELISA Kit

    Cayman Chemical

    Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid in eosinophils, mast cells, and macrophages. Their biosynthesis proceeds via the 5-lipoxygenase (5-LO) pathway in which 5-LO converts arachidonic acid into LTA4 with 5(S)-HpETE as an intermediate. The…

  • The measurement of TG levels, in conjunction with other lipid assays, are useful in the diagnosis of primary and secondary hyperlipoproteinemia, dyslipidemia, and triglyceridemia. Cayman’s TG Assay provides a simple, reproducible, and sensitive tool for assaying TGs in plasma, serum, cell…

  • Prostaglandin E2

    MP Biomedicals

    Prostaglandin E2 is one of the primary cyclooxygenase products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation. The effects of PGE2 are transduced by at…

  • Prostaglandin I (Prostaglandin I2; PGI2) is a potent vasodilator and inhibitor of platelet aggregation that is formed from arachidonic acid primarily in the vascular endothelium and renal cortex by sequential activities of cyclooxygenase (COX) and PGI2 synthase. It is non-enzymatically hydrated to…

  • 17-phenyl trinor PGF2α is a metabolically stable analog of PGF2α and is a potent agonist for the FP receptor. The ethyl amide analog has been approved for use as an ocular hypotensive drug, sold under the Allergan trade name Bimatoprost. There is some evidence that unmetabolized…

  • Prostaglandin D2 ELISA Kit

    Cayman Chemical

    Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kDa glutathione-independent lipocalin-type PGD2 synthase. This PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized in mast cells and leukocytes by a…

  • Thromboxane A2 (TXA2), produced from arachidonic acid, causes irreversible platelet aggregation, vascular, and bronchial smooth muscle contraction. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2. However, most of the TXB2 measured in the plasma or urine of healthy individuals is due to…

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