Tsg Plus is a mixture of Tsg and Proof reading enzyme. Tsg is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0081). Tsg Plus is used to improve the reliability and yield of conventional primer extension reaction.

Taq Plus is a mixture of Taq and Proof Reading enzyme. Taq is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0089). Taq Plus is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages: (1)…

To clone blunt-ended PCR fragments generated by  Pfu DNA polymerase or other enzymes to T-Vector,  A-tailing reaction is required. This kit provides Taq  DNA polymerase and dATP mix for adding single A  to these blunt-ended DNA fragments. A tailed DNA  fragments are…

phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3’ ?5’ exonuclease (proofreading) activity acting preferentially on…

Suitable for cohesive or blunt ends and all common ligation reactions.

T4 DNA Polymerase, a template-depended DNA polymerase, catalyzes 5'?3' synthesis from primed single-stranded DNA. The enzyme has a 3'?5' exonuclease activity, but lacks 5'?3' exonuclease activity.

Tsg DNA Polymerase is a thermostable DNA Polymerase isolated from a strain of Thermus sp. Tsg has a half life of 3 hours at 95C, it is therefore more stable than Tsg DNA Polymerase. Tsg has high fidelity with an error frequency 10 / 106 (or 0.01 / 103) during DNA synthesis. Tsg is designed for use…

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. The T4 DNA Ligase…

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5' 3' synthesis of DNA. The enzyme also exhibits 3' 5' exonuclease (proofreading) activity, 5' 3' exonuclease activity and ribonuclease H activity.

This kit is designed to add a single deoxythymidine (dT) residue at the 3’ end of any blunt-end DNA fragments (normally a vector) based on the non-template-dependent terminal transferase activity of Taq polymerase. This T residue on the vector will hybridize to the single A overhang on the…