DNA Modification Enzymes

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DNA Modification Enzymes

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  • A-Tailing Kit

    Bio Basic Inc.

    A- Tailing Kit: To clone blunt-ended PCR fragments generated by Pfu DNA polymerase or other enzymes to T-Vector,To clone blunt-ended PCR fragments generated by Pfu DNA polymerase or other enzymes to T-Vector, A-tailing reaction is required. This kit provides Taq DNA polymerase and dATP mix for…

  • Tsg DNA Polymerase

    Bio Basic Inc.

    Tsg DNA Polymerase, 5u/ul: Tsg DNA Polymerase, 5u/ul Product information Tsg DNA Polymerase (New Buffer!) Product Description: Tsg DNA Polymerase is a thermostable DNA Polymerase isolated from a strain of Thermus sp. Tsg has a half life of 3 hours at 95°C, it is therefore more…

  • T4 DNA Ligase

    Bio Basic Inc.

    T4 DNA Ligase, 5u/ul: Enzyme Description: T4 DNA Ligase catalyzes the formaon of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either…

  • DNA Polymerase I

    Bio Basic Inc.

    DNA Polymerase I, 10u/ul: DNA Polymerase I, a template-dependent DNA polymerase, catalyzes 5'®3' synthesis of DNA. The enzyme also exhibits 3'®5' exonuclease (proofreading) activity, 5'®3' exonuclease activity and ribonuclease H activity.

  • Enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes. Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides Enzymes…

  • Ready-To-Go T4 DNA Ligase For covalently-linking DNA fragments in as little as 30 min. Single-dose, ambient-temperature-stable reaction mixes. For covalently-linking DNA fragments in as little as 30 min.Single-dose, ambient-temperature-stable reaction mixes.Ready-To-Go T4 DNA Ligase Catalyzes…

  • T4 RNA Ligase

    Bio Basic Inc.

  • Tsg Plus DNA Polymerase

    Bio Basic Inc.

    Tsg Plus DNA Polymerase, 5u/ul: Tsg Plus is a mixture of Tsg and Proof-reading enzyme. Tsg is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0081). Tsg Plus is used to improve the reliability and yield of conventional primer extension reaction.

  • T4 DNA Polymerase

    Bio Basic Inc.

    T4 DNA Polymerase, 5u/ul: T4 DNA Polymerase, a template-depended DNA polymerase, catalyzes 5'→3' synthesis from primed single-stranded DNA. The enzyme has a 3'→5' exonuclease activity, but lacks 5'→3' exonuclease activity.

  • phi29 DNA Polymerase

    Bio Basic Inc.

    PHI29 DNA Polymerase, 10u/ul: phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3’ →5’ exonuclease…

  • Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes. Enzymes optimized to work together for high efficiency removal of unincorporated primers and nucleotides. …

  • Taq Plus DNA Polymerase

    Bio Basic Inc.

    Taq Plus DNA Polymerase, 5u/ul: Taq Plus is a mixture of Taq and Proof Reading enzyme. Taq is a thermostable DNA polymerase isolated from a strain of Thermus sp (see product number D0089). Taq Plus is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has…

  • Alkaline Phosphatase catalyzes the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins. Bacterial alkaline phosphatase recombinantly expressed in E. coli . Active in PCR and restriction endonuclease buffers. Inactivated by heating at 75°C for 5 minutes. Bacterial…

  • Exonuclease I catalyzes the sequential removal of nucleotides from the 3' end of single-stranded DNA molecules. 3'?5' single-stranded exonuclease. Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli . Inactivated by heating at 80°C for 15 minutes. Active in PCR…

  • T4 DNA Ligase with PEG

    Bio Basic Inc.

    T4 DNA Ligase with PEG, 5u/ul: T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt-end and cohesive end termini as well as repair single stranded nicks in duplex DNA,…

  • Fast DNA Ligation Kit

    Bio Basic Inc.

    Fast DNA Ligation Kit (5u/ul, T4): This Fast DNA Ligation Kit is suitable for cohesive or blunt ends and all common ligation reactions.

  • T-Tailing Kit

    Bio Basic Inc.

    T-Tailing Kit: This kit is designed to add a single deoxythymidine (dT) residue at the 3’ end of any blunt-end DNA fragments (normally a vector) based on the non-template-dependent terminal transferase activity of Taq polymerase. This T residue on the vector will hybridize to the single A…

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