Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer.  Linear, double-stranded DNA separates faster in a TAE buffer-based system compared to Tris-Borate-EDTAA (TBE) buffer, but the latter has a higher buffering…

TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis.  TBE has a higher buffering capacity than Tris-Acetate-EDTA (TAE) buffer, but the latter is more suitable for steps involving the action of enzymes on nucleic acids…