Pluronic® F-68 Polyol is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood. Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. A nonionic…

1-Pentanesulfonic Acid Sodium Salt Monohydrate is used as an ion-associating reagent for HPLC analysis of peptides and proteins. Sodium pentanesulfonate is utilized in the analysis of small organic molecular compounds, pharmaceutical products and metabolites, and peptides by such techniques as RP -…

N-Lauroylsarcosine is an anionic detergent. N-Lauroylsarcosine is used for solubilization and separation of membrane proteins and glycoproteins; reported to inhibit hexokinase. It is useful in concentrated salt solutions used in the cell lysis step during RNA purification (helps avoid excessive…

SDS is an anionic detergent used to solubilize and denature protein for Protein Electrophoresis & Blotting. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Surfactant used for…

n-Octyl-β-D-Glucopyranoside is a non-ionic detergent for membrane protein. n-Octyl-glucoside is a non-ionic alkyl-glucoside detergent commonly used in the solubilization of membrane proteins and phospholipids. n-Octyl-glucoside has been used at a molar dependant ratios for the…

Polyethylene glycol (PEG) is a condensation polymer of ethylene oxide and water. Polyethylene Glycol has been used in the precipitation of proteins, as a fusing agent in enhancing the effect of macrophages on hybridoma, in the separation and purification of biomolecules and in induction of cell…

Deoxycholic Acid Sodium Salt Monohydrate is an anionic biological detergent for the solubilization of membrane-bound proteins. Anionic detergent; useful for extraction of membrane receptors and other plasma membrane proteins and for nuclei isolation. Deoxycholic acid has been used in a modified…

TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in…

Polysorbate-20 is a nonionic surfactants derived from sorbitan esters. Polysorbate 20 is used as an emulsifying agent for the preparation of stable oil-inwater emulsions in pharmaceuticals, cosmetics, and cleaning compounds. Polysorbate 20 has been used in preextraction of membranes to remove…

N-Lauroylsarcosine is a derivative of sarcosine. It is an anionic surfactant. N-Lauroylsarcosine is an anionic detergent. It is used for solubilization and separation of membrane proteins and glycoproteins; reported to inhibit hexokinase. It is useful in concentrated salt solutions used in the…

CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. CHAPS is commonly used for protein solubilization in isoelectric focusing and…

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. Triton X-100 is a non-ionic surfactant and…

BRIJ 35 is a non-ionic detergent. BRIJ 35 is useful for the extraction of membrane proteins. For use in Stein-Moore Chromatography and all molecular biology applications. Typically used in the range of 0.1-1%.

Pluronic® F-68 is a non-ionic surfactant in cell culture used to control shear forces in suspension cultures against possible damage during freezing and thawing, transfer and stirring. It can also be used to reduce foaming in stirred cultures and reduce cell attachment to glass. …

Lubrol is a compound composed of ethylene oxide condensates of fatty alcohols. Lubrol is used as a solubilizing agent for membrane bound proteins and membrane-bound adenylcyclase.

Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble…

Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble…

Polysorbate 20 is nonionic surfactant derived from sorbitan ester. Soluble or dispersible in water but differ widely in organic and oil solubilities. Used as an emulsifying agent for the preparation of stable oil-in-water emulsions. Used in pre-extraction of membranes to remove peripheral…

N-Lauroylsarcosine is an anionic detergent. N-Lauroylsarcosine is used for solubilization and separation of membrane proteins and glycoproteins; reported to inhibit hexokinase. It is useful in concentrated salt solutions used in the cell lysis step during RNA purification (helps avoid excessive…

SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

Glyceryl Monooleate is a surfactant that releases free glycerol and oleic acid upon hydrolysis. Glyceryl Monooleate has been used in liquid crystal studies and research shows that in the presence of monoolein, the penetration of the drug cisplatin is doubled. Monoolein (1-Oleoyl-rac-glycerol) is…

Isopropyl b-D-1-thiogalactopyranoside is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repression of the b-galactosidase coding lac gene. …

Taurocholic Acid Sodium Salt is the conjugation product of cholic acid with taurine and the principal constituent of the bile of carnivorous animals. Taurocholic Acid Sodium Salt is an anionic detergent used for protein solubilization. Non-sulfated bile salt, physiological transport substrate…

Methylbenzethonium chloride has been used in a study to assess stem cell death-inducing small molecules. It has also been used in a study to investigate the anti-leishmanial activity of paromomycin ointment (Leshcutan) combined with the immunomodulator Imiquimod.