Detergents

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  • NP40 (Nonidet P-40)

    MP Biomedicals

    Nonidet P-40 is a non-ionic detergent used in the pre-treatment solution of slides prior to in situ hybridization. The detergent helps to reduce background or non-specific binding of a probe by reducing debris. NP40 can also be used in the aqueous post-hybridization washing solution and helps to…

  • Sodium Dodecyl Sulfate

    MP Biomedicals

    SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

  • Pluronic® F-68 Polyol

    MP Biomedicals

    Pluronic® F-68 Polyol is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood. Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. A nonionic…

  • Pluronic® F-68 Polyol, 10% Solution

    MP Biomedicals

    Pluronic® F-68 is a non-ionic surfactant in cell culture used to control shear forces in suspension cultures against possible damage during freezing and thawing, transfer and stirring. It can also be used to reduce foaming in stirred cultures and reduce cell attachment to glass. …

  • Cetyltrimethylammonium Bromide

    MP Biomedicals

    Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble…

  • Taurocholic Acid Sodium Salt

    MP Biomedicals

    Taurocholic Acid Sodium Salt is the conjugation product of cholic acid with taurine and the principal constituent of the bile of carnivorous animals. Taurocholic Acid Sodium Salt is an anionic detergent used for protein solubilization. Non-sulfated bile salt, physiological transport substrate…

  • Sodium Dodecyl Sulfate

    MP Biomedicals

    SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

  • Tetamethylammonium Hydroxide Pentahydrate

    MP Biomedicals

    Tetramethylammonium hydroxide (TMAH or TMAOH) is a quaternary ammonium salt. Tetramethylammonium hydroxide pentahydrate is a clathrate hydrate which has been found to be a fast “proton” or “hydroxide ion” conductor. Tetramethylammonium Hydroxide Pentahydrate is an ion…

  • Isopropyl-B-D-Thiogalactopyranoside

    MP Biomedicals

    Isopropyl b-D-1-thiogalactopyranoside is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repression of the b-galactosidase coding lac gene. …

  • BRIJ 35

    MP Biomedicals

    BRIJ 35 is a non-ionic detergent. BRIJ 35 is useful for the extraction of membrane proteins. For use in Stein-Moore Chromatography and all molecular biology applications. Typically used in the range of 0.1-1%.

  • Glyceryl Monooleate

    MP Biomedicals

    Glyceryl Monooleate is a surfactant that releases free glycerol and oleic acid upon hydrolysis. Glyceryl Monooleate has been used in liquid crystal studies and research shows that in the presence of monoolein, the penetration of the drug cisplatin is doubled. Monoolein (1-Oleoyl-rac-glycerol) is…

  • N-Lauroylsarcosine Sodium Salt, Ultra Pure

    MP Biomedicals

    N-Lauroylsarcosine is a derivative of sarcosine. It is an anionic surfactant. N-Lauroylsarcosine is an anionic detergent. It is used for solubilization and separation of membrane proteins and glycoproteins; reported to inhibit hexokinase. It is useful in concentrated salt solutions used in the…

  • Cetyltrimethylammonium Bromide

    MP Biomedicals

    Cetyltrimethylammonium bromide is a phase transfer catalyst ideal for the precipitation of DNA. Hexadecyltrimethylammonium bromide is a bactericidal, cationic detergent. Its activity is neutralized by soaps and anionic detergents (i.e., SDS). Trimethylammonium bromide compounds form insoluble…

  • N-Lauroylsarcosine Sodium Salt

    MP Biomedicals

    N-Lauroylsarcosine is an anionic detergent. N-Lauroylsarcosine is used for solubilization and separation of membrane proteins and glycoproteins; reported to inhibit hexokinase. It is useful in concentrated salt solutions used in the cell lysis step during RNA purification (helps avoid excessive…

  • Lubrol

    MP Biomedicals

    Lubrol is a compound composed of ethylene oxide condensates of fatty alcohols. Lubrol is used as a solubilizing agent for membrane bound proteins and membrane-bound adenylcyclase.

  • Digitonin

    MP Biomedicals

    Digitonin is a mild nonionic detergent. It permeabilizes plasma membranes of eukaryotic cells by complexing with membrane cholesterol and other unconjugated β-hydroxysterols. Digitonin has following uses: Cholesterol Determination. Use of digitonin-permeabilized adipocytes for cAMP…

  • Triton® X-100

    MP Biomedicals

    Triton® X-100 is a alkylaryl polyether alcohol. It is a nonionic surfactant prepared by the reaction of octylphenol with ethylene oxide. The number ("-100") relates only indirectly to the number of ethylene oxide units in the structure. Triton X-100 is a non-ionic surfactant and…

  • N-Dodecyl-ß-D-Maltoside

    MP Biomedicals

    Dodecyl-β-D-maltoside is a non-ionic detergent similar to n-octyl-β-D-glucopyranoside; however, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution. N-dodecyl-β-D-maltoside is used as a non-ionic detergent for stabilization…

  • Sodium Dodecyl Sulfate

    MP Biomedicals

    SDS is an anionic detergent and wetting agent that is effective in both acid and alkaline solutions. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The…

  • Deoxycholic Acid Sodium Salt Monohydrate

    MP Biomedicals

    Deoxycholic Acid Sodium Salt Monohydrate is an anionic biological detergent for the solubilization of membrane-bound proteins. Anionic detergent; useful for extraction of membrane receptors and other plasma membrane proteins and for nuclei isolation. Deoxycholic acid has been used in a modified…

  • Polysorbate 20

    MP Biomedicals

    Polysorbate-20 is a nonionic surfactants derived from sorbitan esters. Polysorbate 20 is used as an emulsifying agent for the preparation of stable oil-inwater emulsions in pharmaceuticals, cosmetics, and cleaning compounds. Polysorbate 20 has been used in preextraction of membranes to remove…

  • N-Lauroylsarcosine, Sodium Salt

    MP Biomedicals

    N-Lauroylsarcosine is an anionic detergent. N-Lauroylsarcosine is used for solubilization and separation of membrane proteins and glycoproteins; reported to inhibit hexokinase. It is useful in concentrated salt solutions used in the cell lysis step during RNA purification (helps avoid excessive…

  • Sodium Dodecyl Sulfate Solution

    MP Biomedicals

    SDS is an anionic detergent used to solubilize and denature protein for Protein Electrophoresis & Blotting. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Surfactant used for…

  • n-Octyl-ß-D-Glucopyranoside

    MP Biomedicals

    n-Octyl-β-D-Glucopyranoside is a non-ionic detergent for membrane protein. n-Octyl-glucoside is a non-ionic alkyl-glucoside detergent commonly used in the solubilization of membrane proteins and phospholipids. n-Octyl-glucoside has been used at a molar dependant ratios for the…

  • Methyl Benzethonium Chloride

    MP Biomedicals

    Methylbenzethonium chloride has been used in a study to assess stem cell death-inducing small molecules. It has also been used in a study to investigate the anti-leishmanial activity of paromomycin ointment (Leshcutan) combined with the immunomodulator Imiquimod.

  • Polyethylene Glycol

    MP Biomedicals

    Polyethylene glycol (PEG) is a condensation polymer of ethylene oxide and water. Polyethylene Glycol has been used in the precipitation of proteins, as a fusing agent in enhancing the effect of macrophages on hybridoma, in the separation and purification of biomolecules and in induction of cell…

  • CHAPS

    MP Biomedicals

    CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. CHAPS is commonly used for protein solubilization in isoelectric focusing and…

  • Polysorbate 20

    MP Biomedicals

    Polysorbate 20 is nonionic surfactant derived from sorbitan ester. Soluble or dispersible in water but differ widely in organic and oil solubilities. Used as an emulsifying agent for the preparation of stable oil-in-water emulsions. Used in pre-extraction of membranes to remove peripheral…

  • 2-n-Dodecylfuran

    MP Biomedicals

  • 1-Pentanesulfonic Acid Sodium Salt Monohydrate

    MP Biomedicals

    1-Pentanesulfonic Acid Sodium Salt Monohydrate is used as an ion-associating reagent for HPLC analysis of peptides and proteins. Sodium pentanesulfonate is utilized in the analysis of small organic molecular compounds, pharmaceutical products and metabolites, and peptides by such techniques as RP -…

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