(5/6) TAMRA-SE (5-(and -6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides. (5/6) TAMRA absorbs green visible light at 546nm and emits an…

FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and…

Excess dye terminator clean-up post sequencing reaction clean-up is an essential step prior to sequencing. The carry over of excess dye into sequencing results in dye blobs which leads to overwhelming noise and failed calls of sequenced bases and potentially leading to the failure the sequencing…