SOB Medium is the standard media for preparing cells for chemical transformation.  SOC is a special medium for incubating competent cells immediately after transformation to allow expression of transferred resistance genes before exposing cells to selective conditions.

High throughput transformation of yeast cells Simple and fast No need to make competent cells The EZ-Yeast Transformation Kit is designed for high throughput transformation with an efficiency of up to 1,000 colonies per µg plasmid DNA. Suitable for a quick analysis of potentially positive…

High efficiency transformation of yeast cells Process takes less than 90 minutes The Alkali Cation Yeast transformation kit provides all the reagents required to perform a high efficiency transformation of yeast with circular or linear DNA. The entire procedure can be completed in one day and…

Molecular biology grade RNase, DNase and Protease free X-Gal (5-Bromo-4-Chloro-3-Indolyl-β -D-galactopyranoside) is a β -galactosidase chromogenic substrate. In the presence of ß-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate. X-Gal is used for the identification and…

Plasmid DNA isolated from yeast or bacterial cells in ≤30 minutes Proven GENECLEAN® GLASSMILK® technology No phenol/chloroform extractions No ethanol precipitation The Yeast RPM Kit allows isolation of plasmid DNA from both yeast and bacterial cells. It uses a GLASSMILK purification…

IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. IPTG is a galactose analogue not recognized by β-galactosidase. It…

Anti-microbial activity This enzyme purified from chicken egg white is provided as an albumin-free freeze dried powder in the chloride form. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between Nacetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between…

The Yeast Cell Lysis Kit contains the reagents necessary for the yeast cell wall in preparation for efficient lysis with the GNOME® DNA Isolation Kit. It can also be used prior to other traditional DNA purification kits in which reagents alone are not sufficient to lyse yeast cell walls.

SUPER-COMP is an extremely rich medium that significantly increases the competency of the cells when using a simple RbCl/CaCl means of preparing competent host cells. After cells are made competent, they are immediately ready for DNA uptake. For maximum transformation efficiency, store for…