Chaotropic Agents
The single-step technique separates RNA from DNA after extraction with an acidic solution. Chaotropic agents disrupt the structure and stability of biological molecules, including protein and nucleic acid.
Protein modification reagents include urea, which is ideal for breaking hydrogen bone and disrupting the native structure of biomolecules. It also includes guanidine hydrochloride, which is perfect for disrupting interactions holding proteins together.
Formamide is an agent used in molecular biology to denature nucleic acids and can make them more accessible for analysis. Chaotropic agents are precious for the study and manipulation of biomolecules.
This is particularly useful in protein purification, nucleic acid extraction, and DNA sequencing, where the goal is to isolate or manipulate specific biomolecules.
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Guanidine Hydrochloride, Ultra Pure
MP Biomedicals* Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins * Used in the isolation of RNA * Solubilizes insoluble or denatured proteins such as inclusion bodies * Can be used for the recovery of periplasmic proteins
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Guanidine Hydrochloride
G-BiosciencesA selection of strong chemicals to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The chaotropes also act on all proteins to unfold them and alter their three-dimensional structure. …
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Urea, Ultra Pure
MP BiomedicalsUrea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle. Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured…
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Guanidine Thiocyanate
G-BiosciencesFeatures Synonym: Guanidinium thiocyanate CAS#: 593-84-0 Molecular Formula: CH5N3 CHNS Molecular Weight: 118.16
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Guanidine Hydrochloride
MP BiomedicalsGuanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first…
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Urea, Ultra Pure
MP BiomedicalsUrea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle. Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with…
