cDNA & Cloning

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cDNA & Cloning

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  • GreenGlo™ Safe DNA Dye, 20,000X in Water, 500µl

    GleenGlo™

  • DNA (Calf Thymus), sonicated

    GE Healthcare

    Genomic DNA sheared by sonication for use in hybridization experiments. Fragmented DNA with a size range from 200-6000 nucleotides. Purified by phenol extraction and freeze dried. Tested for absence of nuclease. Suitable for blocking non-specific binding sites in hybridization experiments to…

  • DNA (Calf Thymus), activated

    GE Healthcare

    Activated calf thymus DNA at 25 A??? units/ml (treated briefly with DNase I) — for preparing substrate used in the study of DNA polymerases. High-quality DNAs from viral and eukaryotic sources used for preparation of assay reagents. Nonmethylated DNA (at a concentration of 500…

  • dNTP mixture, 40mM (10mM of each), 1x500ul/vial

    Denville

    dNTP mixture 40mM (10mM of each) 1x500µl/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis of DNA…

  • Poly(A)

    GE Healthcare

    Poly(A) is a single-stranded RNA homopolymer. Average chain length of > 200 nucleotides. Ribonuclease tested. Average chain length of > 200 nucleotides.Ribonuclease tested.Poly(A) is a single-stranded RNA homopolymer. Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential…

  • First-Strand cDNA Synthesis Kit

    GE Healthcare

    Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method. For synthesis of single-stranded cDNA prior to…

  • 10x PCR Buffer (15mM MgCI2) for Choice-Taq™ DNA Polymerase, 1.0mL

    Denville

    Choice-Taq™ DNA Polymerase is a recombinant Taq DNA polymerase Choice-Taq™ is supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • Ready-To-Go You-Prime First-Strand Beads

    GE Healthcare

    The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 ml tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. For synthesis of first-strand cDNA templates…

  • DNA Marker, 1 kb Ladder, 13 Fragments, 200 loads

    Denville

    200 loads easy reference select bolded bands 100 bp and 1 kb marker choices Fragments with bolded intensity for easy viewing Ready-to-use format Idea for agarose gel electrophoresis, Northern, and Southern applications

  • Ready-To-Go T-Primed First-Strand Kit

    GE Healthcare

    The kit provides 50 single-dose, ambient-temperature-stable, first-strand cDNA synthesis reactions, each containing NotI-(dT)18 Primer, Cloned FPLC pure M-MuLV Reverse Transcriptase, RNase Inhibitor, and nucleotides. For synthesis of full-length first-strand cDNA templates from…

  • dNTP set dA, dG, dC, & dT, 100mM, 4x250µl/vial

    Denville

    dNTP set dA, dG, dC, & dT 100mM 4x250ul/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis of DNA or…

  • KiloBase DNA Marker

    GE Healthcare

    KiloBase DNA Marker is used for quick and precise sizing of double-stranded DNA fragments from 500 bp to 10.0 kb on agarose gels. For quick and precise sizing of double-stranded DNA fragments from 500 bp to 10.0 kb on agarose gels. Supplied at 0.5 µg/µl in TE buffer (pH 8.0) in sufficient…

  • DNA (Salmon Testes), sonicated

    GE Healthcare

    Genomic DNA sheared by sonication for use in hybridization experiments Fragmented DNA with a size range from 200-6000 nucleotides. Purified by phenol extraction and freeze dried. Tested for absence of nuclease. Suitable for blocking non-specific binding sites in hybridization experiments to…

  • DNA Marker, 100 bp Ladder, 11 Fragments, 500 loads

    Denville

    500 loads Easy reference select bolded bands 100 bp and 1 kb marker choices Fragments with bolded intensity for easy viewing Ready-to-use format Idea for agarose gel electrophoresis, Northern, and Southern applications

  • Real Time PCR mastermix 2X (No ROX)

    Denville

    100 rxn, 1ml Real Time PCR mastermix 2X (No ROX) optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

  • DNA Marker, 100 bp Ladder, 11 Fragments, 200 loads

    Denville

    200 loads Easy reference select bolded bands 100 bp and 1 kb marker choices Fragments with bolded intensity for easy viewing Ready-to-use format Idea for agarose gel electrophoresis, Northern, and Southern applications

  • dNTP mixture, 100mM (25mM of each), 4x500µl/vial

    Denville

    dNTP mixture 100mM (25mM of each) 4x500µl/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis of…

  • PBS 10X PH 7.4 1L

    Hoefer

  • 10x PCR Buffer Mg free for Choice-Taq™ DNA Polymerase, 1.0mL

    Denville

    Choice-Taq™ DNA Polymerase is a recombinant Taq DNA polymerase Choice-Taq™ is supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • SunScript™ One Step RT-qPCR Kits

    Expedeon

    SunScript™ One Step RT-qPCR Kit is an easy and reliable system designed for fast RT-qPCR (real time) reactions based on detection of double-stranded DNA-binding dyes. One key advantage of the kit is the elevated temperature for performing the RT step thus allowing higher specificity of the…

  • dNTP set dA, dG, dC, & dT, 100mM, 20 x 4 x 250ul/vial

    Denville

    dNTP set dA, dG, dC, & dT 100mM 20 x 4 x 250ul/vial

  • SunScript™ Reverse Transcriptase RNaseH-

    Expedeon

    SunScript™ Reverse Transcriptase (RNaseH-) is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity based on the well characterized HIV-1 RT. SunScript can completely synthesise cDNA of up to 11 Kb in length in only 5 minutes. Supply …

  • SunScript™ Reverse Transcriptase RNaseH+

    Expedeon

    SunScript™ Reverse Transcriptase RNaseH+ is a reverse transcriptase that has been engineered for high thermostability based on the well characterized HIV-1 RT. It has RNase activity, unlike the standard SunScript Reverse Transcriptase. SunScript can completely synthesise cDNA of up to 11 Kb…

  • SunScript™ Reverse Transcriptase

    Expedeon

    SunScript™ Reverse Transcriptase RNaseH- is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity based on the well characterized HIV-1 RT. It has RNA and DNA-dependent polymerase activity. The enzyme is active up to temperatures of 85°C.…

  • GreenGlo™ Safe DNA Dye, 20,000X in Water, 5mL

    GleenGlo™

  • TBS, 20X Solution, pH 7.4

    Hoefer

  • 2x Real-Time PCR Mastermix, Regular Level of Rox

    Denville

    100 rxn, 1ml 2x Real-Time PCR Mastermix Regular Level of Rox Optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye. The user can easily use it to detect and quantify the expression of target genes via Real Time PCR.

  • dNTP mixture, 100mM (25mM of each), 1x500ul/vial

    Denville

    dNTP mixture 100mM (25mM of each) 1x500µl/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis of…

  • DNA Marker, 1 kb Ladder, 13 Fragments, 500 loads

    Denville

    500 loads Easy reference select bolded bands 100 bp and 1 kb marker choices Fragments with bolded intensity for easy viewing Ready-to-use format Idea for agarose gel electrophoresis, Northern, and Southern applications

  • TBE Buffer (10X)

    Hoefer

    TBE Buffer (10X) Ultra Pure Molecular Biology 1L

  • dNTP set dA, dG, dC, & dT, 100mM, 4x4x250µl/vial

    Denville

    dNTP set dA, dG, dC, & dT 100mM 4x4x250µl/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis…

  • DNA Suspension Buffer

    Teknova

    DNA suspension buffer is used to solubilize oligonucleotides, genomic DNA and plasmid DNA. Ingredients: 10mM Tris-HCl 0.1mM EDTA pH 8.0 DNA suspension buffer is also referred to as TElowE or 1TE0.1E. The purpose of this buffer is to solubilize DNA or RNA, while protecting…

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