cDNA & Cloning

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cDNA & Cloning

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Attention Thomas Scientific Customers

Due to current pandemic, lead times for PPE items are longer than what is listed on our website. Please expect shipping delays of all PPE products during this time.

  • DNA Marker, 1 kb Ladder, 13 Fragments, 200 loads

    Denville

    200 loads easy reference select bolded bands 100 bp and 1 kb marker choices Fragments with bolded intensity for easy viewing Ready-to-use format Idea for agarose gel electrophoresis, Northern, and Southern applications

  • Ready-To-Go T-Primed First-Strand Kit

    GE Healthcare

    The kit provides 50 single-dose, ambient-temperature-stable, first-strand cDNA synthesis reactions, each containing NotI-(dT)18 Primer, Cloned FPLC pure M-MuLV Reverse Transcriptase, RNase Inhibitor, and nucleotides. For synthesis of full-length first-strand cDNA templates from…

  • Real Time PCR mastermix 2X (No ROX)

    Denville

    100 rxn, 1ml Real Time PCR mastermix 2X (No ROX) optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

  • DNA (Calf Thymus), activated

    GE Healthcare

    Activated calf thymus DNA at 25 A??? units/ml (treated briefly with DNase I) — for preparing substrate used in the study of DNA polymerases. High-quality DNAs from viral and eukaryotic sources used for preparation of assay reagents. Nonmethylated DNA (at a concentration of 500…

  • 10x PCR Buffer (15mM MgCI2) for Choice-Taq™ DNA Polymerase, 1.0mL

    Denville

    Choice-Taq™ DNA Polymerase is a recombinant Taq DNA polymerase Choice-Taq™ is supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • DNA Marker, 100 bp Ladder, 11 Fragments, 500 loads

    Denville

    500 loads Easy reference select bolded bands 100 bp and 1 kb marker choices Fragments with bolded intensity for easy viewing Ready-to-use format Idea for agarose gel electrophoresis, Northern, and Southern applications

  • KiloBase DNA Marker

    GE Healthcare

    KiloBase DNA Marker is used for quick and precise sizing of double-stranded DNA fragments from 500 bp to 10.0 kb on agarose gels. For quick and precise sizing of double-stranded DNA fragments from 500 bp to 10.0 kb on agarose gels. Supplied at 0.5 µg/µl in TE buffer (pH 8.0) in sufficient…

  • dNTP set dA, dG, dC, & dT, 100mM, 4x250µl/vial

    Denville

    dNTP set dA, dG, dC, & dT 100mM 4x250ul/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis of DNA or…

  • dNTP set dA, dG, dC, & dT, 100mM, 4x4x250µl/vial

    Denville

    dNTP set dA, dG, dC, & dT 100mM 4x4x250µl/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis…

  • dNTP mixture, 40mM (10mM of each), 1x500ul/vial

    Denville

    dNTP mixture 40mM (10mM of each) 1x500µl/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis of DNA…

  • dNTP set dA, dG, dC, & dT, 100mM, 20 x 4 x 250ul/vial

    Denville

    dNTP set dA, dG, dC, & dT 100mM 20 x 4 x 250ul/vial

  • Poly(A)

    GE Healthcare

    Poly(A) is a single-stranded RNA homopolymer. Average chain length of > 200 nucleotides. Ribonuclease tested. Average chain length of > 200 nucleotides.Ribonuclease tested.Poly(A) is a single-stranded RNA homopolymer. Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential…

  • 10x PCR Buffer Mg free for Choice-Taq™ DNA Polymerase, 1.0mL

    Denville

    Choice-Taq™ DNA Polymerase is a recombinant Taq DNA polymerase Choice-Taq™ is supplied with a 10x NH4 Reaction buffer containing 1.5mM MgCI2 (final concentration)

  • DNA Marker, 100 bp Ladder, 11 Fragments, 200 loads

    Denville

    200 loads Easy reference select bolded bands 100 bp and 1 kb marker choices Fragments with bolded intensity for easy viewing Ready-to-use format Idea for agarose gel electrophoresis, Northern, and Southern applications

  • DNA (Calf Thymus), sonicated

    GE Healthcare

    Genomic DNA sheared by sonication for use in hybridization experiments. Fragmented DNA with a size range from 200-6000 nucleotides. Purified by phenol extraction and freeze dried. Tested for absence of nuclease. Suitable for blocking non-specific binding sites in hybridization experiments to…

  • dNTP mixture, 100mM (25mM of each), 4x500µl/vial

    Denville

    dNTP mixture 100mM (25mM of each) 4x500µl/vial Storage Condition: -20ºC constant temperature freezer. Purity: Each dNTP is >99% chromatographically pure. Free of DNase, RNase, Protease, or Phosphatase activities. Application: For direct in-vitro synthesis of…

  • SunScript™ One Step RT-qPCR Kits

    Expedeon

    SunScript™ One Step RT-qPCR Kit is an easy and reliable system designed for fast RT-qPCR (real time) reactions based on detection of double-stranded DNA-binding dyes. One key advantage of the kit is the elevated temperature for performing the RT step thus allowing higher specificity of the…

  • DNA (Salmon Testes), sonicated

    GE Healthcare

    Genomic DNA sheared by sonication for use in hybridization experiments Fragmented DNA with a size range from 200-6000 nucleotides. Purified by phenol extraction and freeze dried. Tested for absence of nuclease. Suitable for blocking non-specific binding sites in hybridization experiments to…

  • First-Strand cDNA Synthesis Kit

    GE Healthcare

    Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method. For synthesis of single-stranded cDNA prior to…

  • Ready-To-Go You-Prime First-Strand Beads

    GE Healthcare

    The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 ml tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. For synthesis of first-strand cDNA templates…

  • SunScript™ Reverse Transcriptase RNaseH+

    Expedeon

    SunScript™ Reverse Transcriptase RNaseH+ is a reverse transcriptase that has been engineered for high thermostability based on the well characterized HIV-1 RT. It has RNase activity, unlike the standard SunScript Reverse Transcriptase. SunScript can completely synthesise cDNA of up to 11 Kb…

  • SunScript™ Reverse Transcriptase RNaseH-

    Expedeon

    SunScript™ Reverse Transcriptase (RNaseH-) is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity based on the well characterized HIV-1 RT. SunScript can completely synthesise cDNA of up to 11 Kb in length in only 5 minutes. Supply …

  • SunScript™ One Step RT-PCR Kits

    Expedeon

    A key advantage of SunScript is the elevated temperature for performing the RT step thus allowing higher specificity of the reaction, and a higher likelihood in obtaining reaction products for difficult targets. Moreover, the excellent kinetic performance of the SunScript™ Reverse…

  • SunScript™ Reverse Transcriptase

    Expedeon

    SunScript™ Reverse Transcriptase RNaseH- is a reverse transcriptase that has been engineered for high thermostability and absence of RNaseH activity based on the well characterized HIV-1 RT. It has RNA and DNA-dependent polymerase activity. The enzyme is active up to temperatures of 85°C.…

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