Product contains enough liquid concentrate to make 1L of 1X solution. To use this product, dilute it 4 fold with DI water and add 0.6% beta ME. Wash membrane in this buffer and incubate at 65°C for 30 minutes under constant agitation. Wash the membrane 5X for 5 min in PBS-Tween20 or…

NFDM Diluent/Blocking 10X, in a priority physiological saline borate buffer is used to minimize non-specific binding of antibody. Technical Information: pH 7.6 Sold as 10 x 100mL.

Blot blocking buffer used to block non-specific binding site for antibodies on the membrane. Technical Information: 10% Horse serum, 0.02% sodium azide in PBS