Buffers

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  • Components: PIPES(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(100 mM), Sodium Phosphate Monobasic(20 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Chloride(1.17 M), Sodium Bicarbonate(260 mM), Dextrose / Glucose(56 mM), Potassium Chloride(53 mM), Sodium Phosphate Monobasic(10 mM), Phenol Red(1 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ±…

  • Components: Potassium Phosphate Monobasic / Potassium Phosphate Dibasic(200 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • HEPES Buffer (1 M, pH 7.4)

    Boston BioProducts

    Components: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.5 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Tris Buffered Saline-Tween

    Boston BioProducts

    Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Tween-20(0.05%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Components: Sodium Chloride(140 mM), Potassium Chloride(5 mM), HEPES(25 mM), Calcium Chloride Dihydrate(2 mM), Magnesium Chloride Hexahydrate(2 mM), Dextrose / Glucose(10 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4…

  • Components: EDTA(~0.74%), Tris Base / Tris-HCl(~3.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • HEPES Buffer (0.5 M, pH 7.4)

    Boston BioProducts

    Components: HEPES(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(101.4 mM), Potassium Phosphate Monobasic(17.6 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Chloride(127 mM), Potassium Chloride(5 mM), Sodium Phosphate Monobasic(1.8 mM), Magnesium Chloride(1 mM), Sodium Bicarbonate(25 mM), Dextrose / Glucose(10 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4…

  • Components: Sodium Chloride(127 mM), Potassium Chloride(5 mM), Sodium Phosphate Monobasic(1.8 mM), Magnesium Chloride Hexahydrate(1 mM), Calcium Chloride Dihydrate(1 mM), Sodium Bicarbonate(25 mM), Dextrose / Glucose(5.5 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and…

  • Components: Sodium Phosphate Dibasic(8 mM), Potassium Phosphate Monobasic(2 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(10 mM), Potassium Phosphate Monobasic(2 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Triton X-100(0.3%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 …

  • Components: HEPES(1 M), Potassium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: Sodium Chloride(1.37 M), Potassium Phosphate Monobasic(14.7 mM), Sodium Phosphate Dibasic(80.6 mM), Potassium Chloride(26.7 mM) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Components: Tris Base / Tris-HCl(200 mM), EDTA(20 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Chloride(137.93 mM), Sodium Phosphate Dibasic Heptahydrate(8.06 mM), Potassium Chloride(2.67 mM), Potassium Phosphate Monobasic(1.47 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 …

  • RIPA Buffer (2X)

    Boston BioProducts

    Components: Tris Base / Tris-HCl(100 mM), Sodium Chloride(300 mM), Nonidet P-40 Substitute(2%), Sodium Deoxycholate(1%), Sodium Dodecyl Sulfate (SDS)(0.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) …

  • RIPA Buffer

    Boston BioProducts

    Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Nonidet P-40 Substitute(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(80 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: 2-8°C

  • Components: Sodium Phosphate Dibasic(10 mM), Potassium Phosphate Monobasic(2 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Triton X-100(0.1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 …

  • Components: Sodium Chloride(140 mM), Potassium Chloride(5 mM), HEPES(10 mM), Dextrose / Glucose(10 mM), Sodium Pyruvate(10 mM), BAPTA(2 mM) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: Citric Acid / Sodium Phosphate Dibasic(100 mM / 200 mM mixture), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Components: HEPES(125 mM), Sodium Chloride(750 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • RIPA Buffer (with EGTA)

    Boston BioProducts

    Components: Tris Base / Tris-HCl( 50 mM), Sodium Chloride(150 mM), Nonidet P-40 Substitute(1%), Sodium Deoxycholate(0.5%), EGTA(1 mM), Sodium Dodecyl Sulfate (SDS)(0.1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ±…

  • Components: Sodium Phosphate Dibasic(100 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Triton X-100(1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) …

  • Components: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.

  • Components: Sodium Chloride(1.379 M), Sodium Phosphate Dibasic(80.6 mM), Potassium Chloride(26.7 mM), Potassium Phosphate Monobasic(14.7 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.

  • Components: Sodium Phosphate Dibasic(10 mM), Potassium Phosphate Monobasic(2 mM), Potassium Chloride(2.7 mM), Sodium Chloride(137 mM), Triton X-100(1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage:…

  • Components: HEPES(500 mM), Potassium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

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