Rockland Immunochemicals Inc Buffer Solutions
-
TMB MEMBRANE PEROXIDASE SUBSTRATE. READY-TO-USE, 1L, Liquid - clear
Rockland Immunochemicals IncTMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB…
-
Express Transfer Buffer, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncExpress Transfer Buffer is a alternative to tris-glycine as a transfer buffer in the western blot. It may run at an elevated temperature but gives markedly lower background.
-
10% (w/v) Sodium Dodecyl Sulfate (SDS), 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly…
-
1X SDS-PAGE Destain Solution (Ready-to Use), 1L, Liquid
Rockland Immunochemicals Inc1X SDS-PAGE Destain Solution is ready-to-use as a working solution and requires no further dilution.
-
0.5 M EDTA pH 8.0, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncEthylenediaminetetraacetic acid is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.5 M Trisodium EDTA at pH 8.0. Meticulously prepared using ultra pure reagents dissolved…
-
NORMAL RAT SERUM (NRS), 10mL, Lyophilized
Rockland Immunochemicals IncNormal Rat Serum (NRS) is a useful reagent for blocking Western Blots and ELISA wells.
-
FETAL BOVINE SERUM, Certified, Heat Inactivated Origin: United States triple 0.1…
Rockland Immunochemicals IncHeat inactivated fetal bovine serum is for in vitro diagnostic use, not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. Certified Fetal Bovine Sera meets USDA requirements for abattoir-sourced animals, traceability and country of…
-
TMB ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.
-
NPP (50X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 10mL, Liquid - clear to pale…
Rockland Immunochemicals IncMust be diluted
-
1X Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncChill to 2 to 8 °C prior to use. Lysis Buffer is provided as a ready-to-use 1X solution containing 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4. No preparation is required prior to use.
-
Blocking Buffer Sampler Kit, 1Kit, Liquid and Lyophilized
Rockland Immunochemicals IncBlocking Buffer Sampler Kit contains: NORMAL GOAT SERUM (NGS), BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free), Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - Designed for ELISA assays, 10X…
-
2X SDS-PAGE Sample Buffer, 1.5mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue. The pH of…
-
10X HDAC Assay Buffer, 20mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. Diluted (1X) HDAC Assay buffer consists of 25 mM Tris Cl, 137 mM NaCl, 2.7 mM KCl and 1.0 mM MgCl2 at pH 8.0.
-
-
1.0 M Tris HCl pH 7.6, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer…
-
1X TE pH 8.0 (10 mM Tris HCl, 1.0 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl and 1.0 mM EDTA at a pH of 8.0. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.
-
NORMAL MOUSE SERUM (NMS), 10mL, Lyophilized
Rockland Immunochemicals IncNormal Mouse Serum (NMS) is a useful reagent for blocking Western Blots and ELISA wells.
-
10% (w/v) Sodium Dodecyl Sulfate (SDS), 100mL, Liquid (sterile filtered)
Rockland Immunochemicals Inc10% SDS is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly polished…
-
TMB Substrate for Western Blot MaxTag™ use with 10 ml of TMB Substrate…
Rockland Immunochemicals IncThis following method is suggested as a guideline for the use for Western Blot with nitrocellulose or PVDF membranes. The TMB Substrate in 100 mL bottle TMBM-101 and the TMB Precipitating Agent in 10 mL bottle TMBM-102 are used together. Mix 9 ml of the TMB Substrate and 1.0 ml of the TMB…
-
10X SDS-PAGE Running Gel Buffer (0.25 M Tris, 1.92 M Glycine, 1.0% SDS pH 8.3),…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required.
-
Blocking Buffer for Fluorescent Western Blotting THIMEROSAL FREE 10-PACK (10 x…
Rockland Immunochemicals IncOptimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance. This buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary…
-
Competent Cell Buffer (60 mM Calcium Chloride, 15% (v/v) Glycerol, 10 mM Tris…
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
-
5.0 M Ammonium Acetate (untitrated) DEPC TREATED, 100mL, Liquid (sterile…
Rockland Immunochemicals Inc5.0 Ammonium Acetate is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains untitrated 5.0 M Ammonium Acetate. Meticulously prepared using ultra pure reagents dissolved in highly…
-
10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in…
-
10X TBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5. A…
-
NORMAL GOAT SERUM (NGS), 10mL, Lyophilized
Rockland Immunochemicals IncGoat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.
-
10X Tris-Borate-EDTA (TBE) (0.9 M Tris-Borate 0.01 M EDTA pH 8.3)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Borate EDTA (TBE) consists of 0.9 M Tris-Borate, 0.01 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure reagents…
-
UniGlow™ Liquid
Rockland Immunochemicals IncUniGlow™ is ready-to-use and no mixing is required. UniGlow™- One Component Chemiluminescent Substrate is for use in microwell or membrane applications. Protect from light - UniGlow™ Substrate is a highly sensitive detection reagent. Equilibrate to room temperature before use and aliquot into a…
-
Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml), 1Each,…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications.…
-
2X SDS-PAGE Sample Buffer without DTT or b-ME (125 mM Tris, 20% Glycerol, 4% SDS…
Rockland Immunochemicals Inc2X SDS-PAGE Sample Loading Buffer is suitable for laboratory assays involved in protein biochemistry. 2X SDS-PAGE Sample Buffer is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample…
-
UltraPure Sterile Water, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncUltra Sterile Water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
-
ProXtract B™ Extraction Buffer
Rockland Immunochemicals IncProXtract B™ Buffer is suitable for protein extraction and easily scalable. Specific conditions should be optimized by the end user. See protocol.