Rockland Immunochemicals Inc Buffer Solutions
-
NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale…
Rockland Immunochemicals IncNPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow, Expiration date is one (1) year from date of opening.
-
1.0 M Tris HCl pH 7.2, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncSuitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M…
-
10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Dilute only prior to immediate use. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium…
-
UniGlow™ Liquid
Rockland Immunochemicals IncUniGlow™ is ready-to-use and no mixing is required. UniGlow™- One Component Chemiluminescent Substrate is for use in microwell or membrane applications. Protect from light - UniGlow™ Substrate is a highly sensitive detection reagent. Equilibrate to room temperature before use and aliquot into a…
-
Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) Staining…
Rockland Immunochemicals IncFluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is adequate for cell staining, washing and dilution in flow cytometry and immunofluorescence experiments.
-
Blocking Buffer
Rockland Immunochemicals IncBlockOut® allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye® 800 and IRDye® 700DX (Licor), Cy2™,…
-
Tris-Acetate-EDTA (TAE)
Rockland Immunochemicals IncTris-Acetate-EDTA (TAE) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Tris-Acetate-EDTA (TAE) consists of 2.0 M Tris-Acetate, 0.05 M EDTA at a pH of 8.3. Meticulously prepared using ultra pure…
-
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncThis product is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to ELISA. After coating with antigen or antibody, wash the contents of the ELISA microwells and add a sufficient volume of ELISA Microwell Blocking Buffer with Stabilizer to each well of the microplate. …
-
BCIP/TNBT MEMBRANE ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to very…
Rockland Immunochemicals IncBCIP/TNBT Membrane Alkaline Phosphatase Substrate is a useful detection reagent for WB, IHC, and in situ hybridization.
-
TMB PRESTAINED RED ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB Prestained Red ELISA Peroxidase Substrate produces a soluble blue end product of ELISA, read at 370nm or 655nm.
-
TMB ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.
-
10X BBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X BBS Fish Gel Concentrate consists of 1.0M boric acid, 1.5M sodium chloride and Fish Gelatin at pH 8.0. A proprietary…
-
1.0 M Tris HCl pH 8.5, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 8.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade (WFI) water. This product…
-
1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED, 1L, Liquid …
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1.0 mM EDTA at a pH of 7.8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.
-
10X PBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X PBS Fish Gel Concentrate consists of 0.2M potassium phosphate, 1.5M sodium chloride and Fish Gelatin at pH 7.2. A…
-
BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk), 500g, Lyophilized
Rockland Immunochemicals IncUse BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a…
-
0.5 M Calcium Chloride, 500mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 0.5M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains 0.5 M Calcium Chloride. Meticulously prepared using ultra pure reagents dissolved in highly polished…
-
ELISA Microwell Coating Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncELISA Microwell Coating Stabilizer is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to preforming ELISA. After the addition of blocking agent, wash the contents of ELISA microwells and add a sufficient volume of ELISA Microwell Coating Stabilizer to each well of the…
-
ALP Conjugate Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals IncDilute your primary or secondary alkaline phosphatase conjugated antibody or other protein or compound with ALP Conjugate Stabilizer to the desired 1X working concentration determined for your conjugate. ALP Conjugate Stabilizer may be used to dilute conjugates when determining titer. In one step…
-
10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED, 1L, Liquid (sterile…
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in…
-
20X SSPE Buffer (3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M…
Rockland Immunochemicals IncThis product is a 20X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4. Meticulously prepared…
-
10X TBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris Buffered Saline consists of 1.0 M Tris HCl and 1.5 M Sodium Chloride, pH 7.5, meticulously prepared using ultra pure reagents…
-
FETAL BOVINE SERUM Origin: United States triple 0.1 micron filtered
Rockland Immunochemicals IncFetal Bovine Sera sourced from the United States is collected exclusively from USDA licensed abattoirs from within the United States. Raw material is processed through 0.1 µm (100nm) filters in compliance with regulatory guidelines. Animals are inspected pre-mortem and post-mortem by USDA…
-
20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC…
Rockland Immunochemicals Inc20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH…
-
1X Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncChill to 2 to 8 °C prior to use. Lysis Buffer is provided as a ready-to-use 1X solution containing 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4. No preparation is required prior to use.
-
1.0 M Tris HCl pH 7.4, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 7.4. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or…
-
Molecular Biology Grade UltraPure Water
Rockland Immunochemicals IncMolecular Biology Grade Ultrapure water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
-
10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20), 1L,…
Rockland Immunochemicals IncTBST is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. May be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
-
Cell Lysis Buffer, 15mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThe buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.
-
Express Transfer Buffer, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncExpress Transfer Buffer is a alternative to tris-glycine as a transfer buffer in the western blot. It may run at an elevated temperature but gives markedly lower background.
-
HRP Conjugate Stabilizer (Azide and Mercury Free), 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a ''ready-to-use'' 1X diluent for Horseradish Peroxidase (HRP) conjugated primary or secondary. This buffer contains buffered saline along with proprietary reagents to stabilize and preserve Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies in solution. A…
-
Blocking Buffer for Immunohistochemistry, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis immunohistochemistry protein blocker is specifically designed for immunohistochemical staining where detection occurs using an immunoenzymatic reaction with substrate, i.e. peroxidase (HRP) with diaminobenzidine (DAB). This product specifically inhibits non-specific staining of IHC detection…