Boston Bioproduct Buffers
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Tris-HCl Buffer (1.0 M, pH 8.0, 0.2 Micron Filtered)
Boston BioProductsComponents: Tris Base / Tris-HCl(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.2 Storage: 2-8°C
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Tris-EDTA Buffer (pH 7.4, Autoclaved)
Boston BioProductsComponents: EDTA(~0.74%), Tris Base / Tris-HCl(~3.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle and/or filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.
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Tris-HCl Buffer (1.0 M, pH 6.8, 0.2 Micron Filtered)
Boston BioProductsComponents: Tris Base / Tris-HCl(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.8 ± 0.2 Storage: 2-8°C
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PIPES Buffer (0.5 M, pH 6.8)
Boston BioProductsComponents: PIPES(500 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.8 ± 0.15 Storage: 2-8°C
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RIPA Buffer (with EDTA)
Boston BioProductsComponents: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), NP-40(1%), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), EDTA(5 mM) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
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Tris-Buffered Saline (20X)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Potassium Chloride(54 mM), Sodium Chloride(2.74 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
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Sodium Phosphate Buffer (0.2 M, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(0.2 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: Room Temp.
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Tris-Buffered Saline (10X, pH 7.4, Low Endotoxin)
Boston BioProductsComponents: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
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Citrate Phosphate Buffer (Mcilvaine Buffer, pH 6.0)
Boston BioProductsComponents: Citric Acid / Sodium Phosphate Dibasic(100 mM / 200 mM mixture), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.0 ± 0.15 Storage: 2-8°C
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Sodium Phosphate Buffer (1 M, pH 7.2) - 50mL
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.2 ± 0.15 Storage: Room Temp.
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HEPES Buffer (1 M, pH 7.4)
Boston BioProductsComponents: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
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Tris Buffered Saline-Tween (20X, with 2% Tween-20, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(1 M), Sodium Chloride(3 M), Tween-20(2%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
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RIPA Buffer (1X, with Triton X)
Boston BioProductsComponents: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Triton X-100(1%), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
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Tris-HCl Buffer (1.0 M, pH 6.5, 0.2 Micron Filtered)
Boston BioProductsComponents: Tris Base / Tris-HCl(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.5 ± 0.2 Storage: 2-8°C
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RIPA Buffer (2X)
Boston BioProductsComponents: Tris Base / Tris-HCl(100 mM), Sodium Chloride(300 mM), Nonidet P-40 Substitute(2%), Sodium Deoxycholate(1%), Sodium Dodecyl Sulfate (SDS)(0.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) …
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Acetate Buffer (1 M, pH 5.0)
Boston BioProductsComponents: Acetic Acid / Sodium Acetate(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 5.0 ± 0.15 Storage: Room Temp.
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HEPES Buffer (0.5 M, pH 7.5)
Boston BioProductsComponents: HEPES(500 mM), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.15 Storage: 2-8°C
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MES Buffer (0.5 M, pH 6.5)
Boston BioProductsComponents: MES Monohydrate(500 mM), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.5 ± 0.15 Storage: 2-8°C
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Tris-HCl Buffer (1.0 M, pH 7.5, 0.2 Micron Filtered)
Boston BioProductsComponents: Tris Base / Tris-HCl(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.5 ± 0.2 Storage: 2-8°C
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Tris Buffered Saline-Tween (10X, with 0.5% Tween-20, pH 7.4)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M), Tween-20(0.5 %) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
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Tris-Buffered Saline (10X, pH 7.6)
Boston BioProductsComponents: Tris Base / Tris-HCl(250 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.6 ± 0.15 (1X) Storage: Room Temp.
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Tris-Buffered Saline (10X, for Western Blot Washing)
Boston BioProductsComponents: Tris Base / Tris-HCl(500 mM), Sodium Chloride(1.5 M) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 1-micron filter. pH: 7.4 ± 0.15 (1X) Storage: Room Temp.
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HEPES Buffer (1 M, pH 7.4, pH Adjusted with KOH)
Boston BioProductsComponents: HEPES(1 M), Potassium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water, pH adjusted with KOH and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C
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Phosphate Buffered Saline-Tween (10X, with 1% Tween-20, pH 7.4)
Boston BioProductsComponents: Sodium Phosphate Dibasic(100 mM), Potassium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Tween-20(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 7.4 ± 0.15…
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EGTA (0.5 M, pH 8.0, Autoclaved)
Boston BioProductsComponents: EGTA(0.5 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and autoclaved at 15 psi (121°C) for 30 minutes on liquid cycle. pH: 8.0 ± 0.15 Storage: Room Temp.
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Tyrode's Solution (with HEPES & Calcium)
Boston BioProductsComponents: Sodium Chloride(140 mM), Potassium Chloride(5 mM), HEPES(25 mM), Calcium Chloride Dihydrate(2 mM), Magnesium Chloride Hexahydrate(2 mM), Dextrose / Glucose(10 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4…
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Sodium Phosphate Buffer (1 M, pH 7.6)
Boston BioProductsComponents: Sodium Phosphate Monobasic / Sodium Phosphate Dibasic(1M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.6 ± 0.15 Storage: Room Temp.
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Citrate Buffer (0.5 M, pH 6.0)
Boston BioProductsComponents: Citric Acid / Sodium Citrate(0.5 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 6.0 ± 0.15 Storage: 2-8°C
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Acetate Buffer (1 M, pH 4.0)
Boston BioProductsComponents: Acetic Acid / Sodium Acetate(1 M), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 4.0 ± 0.15 Storage: Room Temp.
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Tris-EDTA Buffer (pH 8.0, Low Endotoxin)
Boston BioProductsComponents: Tris Base / Tris-HCl(10 mM), EDTA(1 mM), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 Storage: Room Temp.
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HEPES Buffer (1 M, pH 7.0)
Boston BioProductsComponents: HEPES(1 M), Sodium Hydroxide(-), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: 2-8°C
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Phosphate Buffered Saline (10X, pH 7.4, Low Endotoxin)
Boston BioProductsComponents: Sodium Phosphate Dibasic(100 mM), Sodium Phosphate Monobasic(20 mM), Potassium Chloride(27 mM), Sodium Chloride(1.37 M), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 low endotoxin water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) Storage: 2-8°C