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Buffers
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2x RIPA Buffer II
Bio Basic Inc.2 x RIPA Buffer II (pH 8.0) 2x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is…
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CAPSO, Sodium Salt
Bio Basic Inc.CAPSO, sodium salt: Molecular Formula: C9H18NO4SNa Molecular Weight: 259.30 The most common buffer for the blotting of proteins to nitrocellulose is the Tris-Glycine buffer (pH 8.3). The transfer of strongly basic proteins is very poor (~20 %). The use of more alkaline transfer buffers (e.g. 25 mM…
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Bacterial Protein Lysis Buffer, 1x Stength Solution
Bio Basic Inc.Bacterial Protein Lysis Buffer, 1x Stength Solution: Bacterial Protein Lysis Buffer enables mild extraction of proteins from bacteria (E. coli) without the need for mechanical disruption. The reagent may be used for soluble protein extraction and inclusion body purgation from bacterial cell…
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TAE Buffer, Solution
Bio Basic Inc.TAE buffer 25x solution: TAE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but dsDNA runs faster with TAE buffer. This 25x and 50x concentrate can be easily diluted with molecular biology grade…
Related Products: Tae Buffer
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TAE Buffer Premix Powder
Bio Basic Inc.TAE buffer premix powder: Each pack makes 1 L 1x25 TAE buffer or 25 liters of 1X TAE buffer. A single strength solution contains: 0.04 M Tris base 0.04 M Acetic acid 0.001 M EDTA
Related Products: Tae Buffer
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Inclusion Body Wash Buffer
Bio Basic Inc.Inclusion Body Wash Buffer: As many as 70-80% of proteins produced by recombinant techniques in E. coli form inclusion bodies (i.e., protein aggregates). Apart from protein of interest, the inclusion body contains impurities, such as RNA,DNA, Lipopolysaccharide. To prepare high purity…
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2x RIPA Buffer III
Bio Basic Inc.2 x RIPA Buffer IIII with EDTA and EGTA (pH 7.4) 2x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and…
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TG-SDS Buffer (Tris-Glycine-SDS), 10x Solution
Bio Basic Inc.Related Products: Tris Buffer Solution
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TBS Buffer (Tris-Buffered Saline), 20x Solution
Bio Basic Inc.Grade: Cell Culture DG: No Storage: 18 to 25C Sterile: No
Related Products: Tris Buffer Solution
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Lysis-EZ G (Non-Denaturing Cell Lysis Buffer)
Bio Basic Inc.Lysis-EZ G Non-denaturing Cell Lysis Buffer, 1X Strength Solution: The Non-denaturing Cell Lysis Buffer contains no-ion detergent, salt, EDTA. The Lysis buffer is able to lyse cells from adherent cultures, suspension cultures as well as animal tissues. The Lysis buffer enables protein…
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RIPA Buffer III
Bio Basic Inc.RIPA Lysis and Extraction Buffer III, 1x Strength Solution: The RIPA Buffer III, is one of the most reliable buffers with middle-strong strength that lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. The buffer contains EDTA, EGTA and enables…
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2x RIPA Buffer I
Bio Basic Inc.2 x RIPA Buffer I (pH 7.4) 2 x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is…
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HEPES, Free Acid
Bio Basic Inc.HEPES, free acid: HEPES has been described as one of the best all-purpose buffers available for biological research. At most biological pHs the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture.…
Related Products: Hepes
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