Bis-Tris is a zwitterionic buffer; an amine buffer similar to Tris Hydrochloric acid that exhibits only a very small shift in dissociation constant with respect to temperature. It is structurally analogous to the the Good buffers that were developed to provide buffers in the pH range of 6.15 - 8.35…

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to…