Assay Kits

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  • This assay system utilizes proprietary human cells engineered to provide constitutive, highlevel expression of the Rat Pregnane X Receptor (nr1i2), a ligand-dependent transcription factor commonly referred to as PXR. PXR is also known as the Steroid and Xenobiotic sensing nuclear Receptor (SXR).…

  • Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as ‘Host Cell Proteins (HCP)', are generated and need to be removed. This kit…

  • This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive high-level expression of full-length, unmodified human mineralocorticoid receptor (NR3C2), a ligand-dependent transcription factor commonly referred to as MR. INDIGO’s reporter cells…

  • INDIGO’s panel of ER assays utilizes non-human mammalian cells engineered to express human estrogen receptors alpha (ER1; NR3A1) and beta (ER2; NR3A2), ligand-dependent transcription factors commonly referred to as ERα and ERβ. INDIGO’s ER reporter cells include the…

  • DHN-MA EIA Kit

    Cayman Chemical

    Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Measurement of 4-hydroxy-2-nonenal (HNE), an aldehyde formed during the lipid peroxidation process, is one of several accepted…

  • UltraPure Water

    Cayman Chemical

    Water used for the preparation of EIA reagents and buffers.

  • Lyophilized fluorescent substrate.

  • Tris-glycine stock solution (10X) contains filtered Tris-base containing 1.9 M glycine.

  • Obestatin (human) EIA Kit

    Cayman Chemical

    Obestatin is an amidated peptide of 23 amino-acids with an alpha-helical secondary conformation. It was first isolated from rat stomach in 2005. Obestatin is a preproghrelin-derived peptide produced by many tissues or organs, including the stomach, pancreas, adipose tissue, skeletal muscle, and…

  • Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a ubiquitously expressed, basic leucine zipper transcription factor. It regulates the expression of a variety of genes,encoding proteins that play critical roles in cyto-protection, as well as the detoxification and clearance of harmful…

  • This nuclear receptor assay system utilizes proprietary human mammalian cells engineered to provide high-level expression of a hybrid form of the human estrogen-related receptor alpha (NR3B1). The N-terminal DNA binding domains (DBD) of the native ERRα has been substituted with that of the…

  • This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the full-length human progesterone receptor (NR3C3), a ligand-dependent transcription factor commonly referred to as PGR. INDIGO's Reporter Cells include the…

  • For the measurement of acylated ghrelin.

  • This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human constitutive androstane receptor, isoform 3 (NR1I3), a ligand-dependent transcription factor commonly referred to as CAR3. These reporter cells…

  • The thioredoxin (Trx) system is the major protein disulfide reductase in cells and comprises Trx, Trx reductase (TrxR), and NADPH. Trx systems are ubiquitous and are critical for cell viability, proliferation, activation of transcription factors, and control of intracellular redox states. The…

  • Adiponectin (human) EIA Kit

    Cayman Chemical

    This EIA is based on the competition between free adiponectin and coated adiponectin coated to the wells of a 96-well plate for a fixed quantity of HRP-labeled adiponectin antibody.

  • Leptin (mouse/rat) EIA Kit

    Cayman Chemical

    This EIA utilizes plates coated with a polyclonal antibody specific for murine/rat leptin. A biotin-labeled polyclonal antibody and streptavidin-horseradish peroxidase are used for detection.

  • The live cell multiplex (LCM) assay provides an efficient fluorescence-based method of quantifying the relative number of live cells resident in treated wells of an assay plate. While the LCM assay may be performed as a stand-alone assay, it is has been specifically optimized to be run in multiplex…

  • Indomethacin is a COX inhibitor that prevents ex vivo TXB2 formation and other PGs that can not be prevented using anticoagulant alone. The samples have to be transferred in indomethacin sampling tubes as soon as possible after blood collection.

  • Sampling Tubes with PHMB

    Cayman Chemical

    4-(hydroxymercuri)Benzoic Acid(PHMB) is used to inhibit cysteine-protease and acts as a reversible thiol-specific blocker. While blocking the acylated ghrelin degradation, it allows the AcetylCholine Esterase AChE™ used in SPI-Bio EIA kits to work normally, in contrast to serine-protease inhibitors.

  • This nuclear receptor assay system utilizes a proprietary rodent cell line that is further engineered to express the mouse/rat peroxisome proliferator-activated receptor gamma (NR1C3, PPARG). Because the receptor’s ligand binding domain sequence is conserved between mouse and rat species, the…

  • Histamine EIA Kit

    Cayman Chemical

    Cayman’s Histamine EIA is a derivitization-amplified competitive enzyme immunoassay which detects histamine within the range from 40 to 5,500 pg/ml. The assay can be used for the analysis of histamine in blood (plasma or serum) without extraction or purification. The use of other sample types…

  • Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor that is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that control energy homeostasis. Ghrelin is present in the peripheral circulation in two…

  • 20-Hydroxyecdysone EIA Kit

    Cayman Chemical

    For quantification of 20-hydroxyecdysone in a variety of biological samples.

  • Sampling Tubes with BHT

    Cayman Chemical

    BHT is a phenolic antioxidant and free radical scavenger. It has been shown to inhibit lipid peroxidation.

  • This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human vitamin D receptor (NR1I1), a ligand-dependent transcription factor commonly referred to as VDR. INDIGO’s reporter cells include the luciferase reporter…

  • This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, alpha (NR1B1), a ligand-dependent transcription factor commonly referred to as RARA or RARα. INDIGO’s reporter cells…

  • LRH-1, also called NR5A2, is a nuclear receptor that is an essential regulator of gene transcription, critical for early cellular development and imperative for the proper functions of the liver, pancreas, and intestines during the adult life. In the liver, LRH-1 regulates the expression of key…

  • Atriopeptin (rat) EIA Kit

    Cayman Chemical

    Atriopeptin is a 28 amino acid peptide synthesized primarily in cardiac atria. This peptide hormone acts in opposition to angiotensin II in regulating renal, hemodynamic, and endocrine function. Atriopeptin is released in response to the increased pressure and mechanical stretch of the right atrium…

  • This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human farnesoid X receptor (NR1H4), a ligand-dependent transcription factor commonly referred to as FXR. INDIGO’s reporter cells include the…

  • This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the mouse peroxisome proliferator-activated receptor alpha (NR1C1), a ligand-dependent transcription factor commonly referred to as PPARA or mPPARα.…

  • This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoid X receptor gamma (NR2B3), a ligand-dependent transcription factor commonly referred to as RXRG or RXRγ. INDIGO’s reporter cells include…

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