Assay Kits

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  • β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol…

  • Protein dotMETRIC™

    G-Biosciences

    Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein…

  • CB-X™ Protein Assay

    G-Biosciences

    Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers…

  • Assay Strips and Holders

    G-Biosciences

    Assay Strips and Holders: 8-well strips of 200ml flat bottomed wells. Ideal for ELISA assays. Cost effective as individual strips can be used as opposed to a whole 96-well plate. Each holder holds 12 strips, sold separately.

  • CB™ Protein Assay

    G-Biosciences

    An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color…

  • G-Biosciences’ CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing…

  • The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0.5-20µg/ml). The Micro BCA…

  • CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells. Adherent or…

  • Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of…

  • Lumino™ Firefly Luciferase Glow Assay is designed for high throughput screening or quantification of luciferase expression in mammalian cells which is mainly performed by batch processing of 96- and 384- well plates. Lumino™ Firefly Luciferase Glow Assay is homogenous,…

  • The CytoScan™ LDH Cytotoxicity Assay is a colorimetric method of assaying cellular cytotoxicity and is an alternative to 51 Cr release cytotoxicity assays. The kit combines the advantages of reliability and simple evaluation, characteristic of radioisotope release assays, with the…

  • Lumino™ ATP Detection Assay is designed for rapid, convenient and quantitative detection of adenosine-5’-triphosphate (ATP). It’s a bioluminescent assay which is based on requirement of ATP by luciferase enzyme for production of light. ATP Detection Assay has widespread…

  • A highly sensitive, colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents, such as reducing agents, chelating agents,…

  • AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change color in response to the chemical reduction of growth medium due to cell…

  • Apoptosis is a form of programmed cell death which is essential as a normal part of development in multicellular organisms. Apoptosis go awry in some disease states such as Alzheimer’s and cancer. Annexin V-FITC Apoptosis Assay is designed to detect apoptosis. It is based on a property…

  • JC-1 Mitochondrial Membrane Potential Assay comprise of lipophilic cationic fluorescent dye; 5,5,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1). In healthy cells, JC-1 enters the energized mitochondria and form aggregates which change the fluorescent…

  • The CytoScan™-fluoro is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a…

  • NUCLEIC dotMETRIC™

    G-Biosciences

    A 1µl Assay For Rapid Estimation of DNA, RNA and Oligo Concentration NUCLEIC dotMETRIC™ kit provides a quick and accurate method for detecting and measuring DNA, RNA and oligonucleotides. The system uses a unique combination of proprietary reagents and test strips and is an…

  • Protn-Latex™

    G-Biosciences

    Protn-Latex ™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minimum of skill or instrumentation. The test detects as low as 25-40µg protein /gm latex (or…

  • RED 660™ Protein Assay

    G-Biosciences

    RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms commercial colorimetric assays, including Bradford and improved Coomassie/ Bradford assays. RED 660™ Protein Assay offers greater linearity, greater color stability, and greater compatibility with detergents,…

  • Firefly Luciferase Assays are one of the widely used reporter assays to detect and measure gene expression, function and regulation. G-Biosciences Lumino™ Firefly Luciferase Assay is designed for rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme…

  • The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP. The Bicinchoninic Acid…

  • G-Biosciences’ ML (Modified Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The ML Protein Assay is compatible with a wide variety of detergents used in protein research in addition to other common reagents such as EDTA and Tris(see tables 1 and 2).The…

  • Agarose Sealing Solution

    G-Biosciences

    Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on…

  • ATP serves as a marker for cell viability as it is present in all metabolically active cells.Lumino™ Cell Viability Glow Assay is a bioluminescent assay to detect and/or quantify ATP and thus measures the viable cells.The bioluminescent assay is based on requirement of ATP by luciferase…

  • CytoScan™-SRB Cytotoxicity Assay is an accurate and reproducible assay based upon the quantitative staining of cellular proteins by sulforhodamine B (SRB). This assay has been used for high-throughput drug screening at the National Cancer Institute (NCI) (1). Sulforhodamine B is an…

  • Fluoro™ Protease Assay

    G-Biosciences

    The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the…

  • NAD+/NADH Assay [Colorimetric] is designed for sensitive detection of intracellular nucleotides, NADH, NAD+ and their ratio. The kit specifically detects NAD+, NADH and not NADP. This colorimetric assay is based on NAD cycling enzyme mix which increases the sensitivity of detection. Features:…

  • PEROX say ™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe 2+ ) ions to ferric (Fe 3+ ) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH…

  • The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct The study of the lac operon has played an important role in…

  • The Bicinchoninic Acid (BCA) Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the…

  • SPN™ Protein Assays

    G-Biosciences

    A novel protein assay that is suitable for single sample or high throughput protein estimation. The SPN™ and SPN™- htp protein assays are rapid assays that are suitable for as little as 0.5µg protein and are resistant to interference from common laboratory agents. The assays are…

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