Assay Kits

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  • RED 660™ Protein Assay

    G-Biosciences

    RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms commercial colorimetric assays, including Bradford and improved Coomassie/ Bradford assays. RED 660™ Protein Assay offers greater linearity, greater color stability, and greater compatibility with detergents,…

  • Protn-Latex™

    G-Biosciences

    Protn-Latex ™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minimum of skill or instrumentation. The test detects as low as 25-40µg protein /gm latex (or…

  • CB-X™ Protein Assay

    G-Biosciences

    Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers…

  • Apoptosis is a form of programmed cell death which is essential as a normal part of development in multicellular organisms. Apoptosis go awry in some disease states such as Alzheimer’s and cancer. Annexin V-FITC Apoptosis Assay is designed to detect apoptosis. It is based on a property…

  • RNase-DETECT™

    G-Biosciences

    RNase -DETECT™ is a highly reliable and sensitive method to detect RNase contamination, which does not utilize unreliable, tedious and expensive test strips or radioactive methods. With RNase -DETECT™, 10µl of test solution is added to our calibrated RNA substrate vial,…

  • The CytoScan™ LDH Cytotoxicity Assay is a colorimetric method of assaying cellular cytotoxicity and is an alternative to 51 Cr release cytotoxicity assays. The kit combines the advantages of reliability and simple evaluation, characteristic of radioisotope release assays, with the…

  • CB™ Protein Assay

    G-Biosciences

    An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color…

  • The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP. The Bicinchoninic Acid…

  • The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0.5-20µg/ml). The Micro BCA…

  • NUCLEIC dotMETRIC™

    G-Biosciences

    A 1µl Assay For Rapid Estimation of DNA, RNA and Oligo Concentration NUCLEIC dotMETRIC™ kit provides a quick and accurate method for detecting and measuring DNA, RNA and oligonucleotides. The system uses a unique combination of proprietary reagents and test strips and is an…

  • The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct The study of the lac operon has played an important role in…

  • HOOK™ BiotinQuant Kit

    G-Biosciences

    HOOK™ BiotinQuant™ uses HABA (2-(4-Hydroxyphenylazo)benzoic acid/ 2-(4′-Hydroxybenzeneazo)benzoic acid/ 4′-Hydroxyazobenzene-2-carboxylic acid) to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein. The method of biotin incorporation estimation…

  • G-Biosciences’ ML (Modified Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The ML Protein Assay is compatible with a wide variety of detergents used in protein research in addition to other common reagents such as EDTA and Tris(see tables 1 and 2).The…

  • The CytoScan™-fluoro is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a…

  • G-Biosciences’ CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing…

  • ATP serves as a marker for cell viability as it is present in all metabolically active cells.Lumino™ Cell Viability Glow Assay is a bioluminescent assay to detect and/or quantify ATP and thus measures the viable cells.The bioluminescent assay is based on requirement of ATP by luciferase…

  • PEROX say ™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe 2+ ) ions to ferric (Fe 3+ ) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH…

  • ProteSEEKER™

    G-Biosciences

    Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening…

  • TMRE Mitochondrial Membrane Potential Assay quantifies changes in mitochondrial membrane potential of live cells via microplate fluorometry, fluorescence microscopy and flow cytometry. Tetramethylrhodamine, ethyl ester (TMRE) is a cell permeable, positively charged red-orange dye that…

  • The Bicinchoninic Acid (BCA) Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the…

  • Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation. The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second most…

  • Calcein AM or Calcein acetoxymethyl ester is a hydrophobic compound which passes easily through cell membrane into live cells and is used for cell viability assays. The non-fluorescent calcein AM dye is hydrolyzed by cellular esterases to give calcein which is fluorescent and is retained in the…

  • Protein dotMETRIC™

    G-Biosciences

    Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer conditions the protein enters into the matrix of the test strip, binds and saturates as protein…

  • Assay Strips and Holders

    G-Biosciences

    Assay Strips and Holders: 8-well strips of 200ml flat bottomed wells. Ideal for ELISA assays. Cost effective as individual strips can be used as opposed to a whole 96-well plate. Each holder holds 12 strips, sold separately.

  • NAD+/NADH Assay [Colorimetric] is designed for sensitive detection of intracellular nucleotides, NADH, NAD+ and their ratio. The kit specifically detects NAD+, NADH and not NADP. This colorimetric assay is based on NAD cycling enzyme mix which increases the sensitivity of detection. Features:…

  • Reduced glutathione (L-γ-glutamyl-L-cysteinylglycine), a tripeptide, serves as a key antioxidant in animal, plant, fungi and bacteria by providing free thiol. Glutathione exist in reduced (GSH) and oxidized (GSSG; gluthathione disulphide) forms in cells and tissues, and the concentration of…

  • AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change color in response to the chemical reduction of growth medium due to cell…

  • A highly sensitive, colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents, such as reducing agents, chelating agents,…

  • β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol…

  • Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation. The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second…

  • Protease Screening Kit

    G-Biosciences

    Sensitive protease screening is needed to study protease activity present in the sample of interest. The Protease Screening Kit provides you with a simple and quick method for testing your samples for proteolysis. Simply incubate your sample in the reagent provided and obtain results. The…

  • Agarose Sealing Solution

    G-Biosciences

    Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimize reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on…

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