Water used for the preparation of EIA reagents and buffers.

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 2 (NR3A2), a ligand-dependent transcription factor commonly referred to as ERβ. INDIGO’s reporter cells include the…

Aldosterone, a steroid hormone secreted by the adrenal cortex, is the principle mineralocorticoid controlling sodium and potassium balance. Serum aldosterone levels in normal upright adult individuals are generally less than 300 pg/ml. Only a fraction of urinary aldosterone is excreted intact, thus…

Cayman’s XTT Cell Proliferation Assay provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and…

Uric acid (urate) is the end product of human purine metabolism and is mainly excreted in urine. Many factors, including genetic components and acquired factors, such as obesity and alcohol consumption, influence serum uric acid concentrations. Hyperuricemia induces or facilitates gout, kidney…

FFAR4/GPR120 is a G protein-coupled receptor for long chain fatty acids such as DHA and EPA ω-3 fatty acids. It is involved in the regulation of glucose uptake in adipocytes and mediates anti-inflammatory activity in macrophages. Cayman’s FFAR4 (GPR120) Reporter Assay Kit consists of a…

The thioredoxin (Trx) system is the major protein disulfide reductase in cells and comprises Trx, Trx reductase (TrxR), and NADPH. Trx systems are ubiquitous and are critical for cell viability, proliferation, activation of transcription factors, and control of intracellular redox states. The…

Collagen-induced arthritis (CIA) is an experimental autoimmune disease that is widely used as a model of human rheumatoid arthritis (RA) in the study of the pathogenic mechanisms of this disease and for testing potential therapeutics. Circulating levels of anti-type II collagen antibody are…

Creatinine is commonly measured in urine and is a key benchmark for the normalization of a variety of urinary biomarkers. Serum creatinine levels, however, are a useful indicator of renal function and abnormal levels have been implicated in diabetes, cardiovascular, and circulatory diseases.…

Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated…

Indomethacin is a COX inhibitor that prevents ex vivo TXB2 formation and other PGs that can not be prevented using anticoagulant alone. The samples have to be transferred in indomethacin sampling tubes as soon as possible after blood collection.

Cayman’s WST-1 Proliferation Assay is based on the reduction of tetrazolium salt WST-1 to a soluble formazan dye by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation.

Cayman’s Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis…

Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating…

Urinary creatinine levels are commonly used as an index of standardization for a variety of other tests. Measurement of creatinine clearance is also useful in detecting renal disease and estimating the extent of impairment of renal function. The Cayman Chemical Creatinine (urinary) Colorimetric…

For measurement of LDH activity as an indicator of cell death.

Cayman’s HAT Inhibitor Screening Assay provides a fast, fluorescence-based method for evaluating PCAF HAT inhibitors. The procedure requires only three easy steps, all performed in the same microplate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3…

Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing, and chromatin regulation. The SAM dependent MTs use SAM as the enzymatic cofactor. SAM, also known as AdoMet, acts…

SEAP is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as CAT and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. Cayman’s Secreted Alkaline Phosphatase Reporter Gene…

Catalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS production. Cayman’s Catalase Assay utilizes the peroxidatic function of…

Cayman’s SOD Assay is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in a convenient 96-well…

Cayman’s Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating…

HDACs catalyze the hydrolytic removal of acetyl groups from histone lysine residues resulting in chromatin condensation and transcriptional repression of chromosomal DNA. Thus, HDAC inhibition allows the conformation of DNA to be relaxed and transcriptional activation to ensue. Transcriptional…

Urea is the final degradation product of protein and amino acid metabolism. Urea is found in blood and is excreted by the kidney as a component of urine. Besides its role as carrier of waste nitrogen, urea also plays a role in the countercurrent exchange system of the nephrons, allowing for…

Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as ‘Host Cell Proteins (HCP)', are generated and need to be removed. This kit…

This EIA is based on the competition between free adiponectin and coated adiponectin coated to the wells of a 96-well plate for a fixed quantity of HRP-labeled adiponectin antibody.

Caspase-3 plays a central role in the execution of apoptosis. Cayman’s Caspase-3 Fluorescence Assay employs a specific caspase-3 substrate, N-Ac-DEVD-N'-MC-R110, which upon cleavage by active caspase-3, generates a highly fluorescent product that can be measured using excitation and…

Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has therapeutic potential in the metabolic disorders frequently associated with obesity.…

Both DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. The repair processes that are initiated to correct this damage release multiple oxidized guanine species into the urine. Cayman Chemical has developed this immunoassay for the measurement of…

Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (adrenocorticotropic hormone), and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress. Plasma corticosterone…

Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis and…

Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative…