Cayman’s HAT Inhibitor Screening Assay provides a fast, fluorescence-based method for evaluating PCAF HAT inhibitors. The procedure requires only three easy steps, all performed in the same microplate. In the first step of the protocol, HAT is incubated with acetyl-CoA and the histone H3…

Small molecule inhibitors of sEH may hold promise as therapeutics for the treatment of hypertension and vascular inflammation. Cayman’s fluorescence-based sEH Inhibitor Screening Assay provides a convenient method for screening epoxide hydrolase inhibitors. The assay utilizes…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the rat peroxisome proliferator-activated receptor delta (NR1C2), a ligand-dependent transcription factor commonly referred to as PPARD or rPPARδ.…

Complex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately,…

cAMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression.

LSD1 is a histone demethylase whose actions on specific lysine residues alter transcription of chromosomal DNA. It also inhibits the tumor suppressor activity of p53 by demethylating a specific lysine residue. Inhibitors of LSD1 are important tools used to elucidate mechanisms of transcription and…

Cayman’s Cell Cycle Phase Determination provides an easy to use tool for studying the induction and inhibition of cell cycle progression in any cell suspension sample. The assay involves the fixation and permeabilization of the cells of interest, making possible the staining of DNA within…

Cayman’s Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis…

ALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and…

Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting extracellular L-lactate, the end product of glycolysis, in cultured cells. In the assay, lactate dehydrogenase catalyzes the oxidation of lactate to pyruvate, in which the formed NADH reduces a tetrazolium…

Autotaxin (ATX, Ectonucleotide Pyrophosphatase/Phosphodiesterase-2, ENPP-2, Lysophospholipase D) is a secreted lysophospholipase D that catalyzes the hydrolysis of lysophosphatidylcholine (LPC) to generate lysophosphatidic acid (LPA). LPA is a lipid mediator that activates G protein-coupled…

Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including…

The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1α has emerged as an important drug target in breast and prostate cancer,…

Cayman’s Protein Carbonyl Colorimetric Assay is a colorimetric assay for the measurement of oxidized proteins. The carbonyls of protein samples are derivatized using 2,4-dinitrophenylhydrazine. Formation of a Schiff base produces the corresponding hydrazone which can be analyzed…

D(−)-Lactate is a stereoisomer of lactate and is present in blood at only 1-5% the concentration of L(+)-lactate. Exogenous sources of D-lactate include fermented foods such as yogurt, sauerkraut, and pickles. D-lactate is normally produced in the gastrointestinal tract, mainly by…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human peroxisome proliferator-activated receptor gamma (NR1C3), a ligand-dependent transcription factor commonly referred to as PPARG or PPARγ.…

Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO).…

PAD2 is a guanidino-modifying enzyme that catalyzes the conversion of specific arginine residues to citrulline in a calcium-dependent manner. This enzyme has been implicated in several diseases, including rheumatoid arthritis (RA), retinal degeneration, and certain cancers. PAD2 has been shown to…

PAF-AH catalyzes the hydrolysis of the potent biologically-active phospholipid PAF, generating inactive lyso-PAF. Cayman’s PAF-AH assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH. Upon…

Cayman’s Autophagy/Cytotoxicity Dual Staining provides a convenient tool for studying the regulation of autophagy and cytotoxicity at the cellular level. The kit employs MDC, an autofluorescent substance incorporated into multilamellar bodies by both an ion trapping mechanism and the…

PAD4 is a guanidino-modifying enzyme that functions as a transcriptional coregulator catalyzing the conversion of specific arginine residues to citrulline. Substrates for PAD4 include histones H2A, H3, and H4. PAD4 autocitrullinates itself at several sites, inhibiting its enzymatic activity. PAD4…

Atriopeptin is a 28 amino acid peptide synthesized primarily in cardiac atria. This peptide hormone acts in opposition to angiotensin II in regulating renal, hemodynamic, and endocrine function. Atriopeptin is released in response to the increased pressure and mechanical stretch of the right atrium…

Cayman’s MPO Inhibitor Screening Assay provides convenient fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. Sufficient reagents are provided for a full 96-well plate assay of each type of activity.

Glutathione (GSH) is involved in maintenance of protein sulfhydryl reduction status. PSSG may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. Cayman’s PSSG provides a convenient method for the direct visualization…

Cayman’s Acid Phosphatase Assay provides a convenient method for detecting AP activity in plasma, serum, urine, and semen. The assay utilizes para-nitrophenyl phosphate (pNPP) as a chromogenic substrate for the enzyme. In the first step, AP dephosphorylates pNPP. In the second step, the…

This EIA is based on a double-antibody sandwich technique which utilizes a murine adiponectin-specific monoclonal capture antibody and a HRP-conjugated polyclonal antibody for detection.

Creatinine is commonly measured in urine and is a key benchmark for the normalization of a variety of urinary biomarkers. Serum creatinine levels, however, are a useful indicator of renal function and abnormal levels have been implicated in diabetes, cardiovascular, and circulatory diseases.…

For the measurement of acylated ghrelin.

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide high-level expression of a hybrid form of the Human Estrogen-Related Receptor γ (NR3B3). The N-terminal DNA binding domains (DBD) of the native ERRγ has been substituted with that of…

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 2 (NR3A2), a ligand-dependent transcription factor commonly referred to as ERβ. INDIGO’s reporter cells include the…

The 20S proteasome is the proteolytic core of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects, evidence that the proteasome may be an important drug target for the treatment for cancer and inflammatory diseases.…

2-Methoxyestradiol (2-ME2) is a natural metabolite of estradiol with potent antitumor and antiangiogenic properties. 2-Methoxyestradiol is a natural metabolite of estradiol with potent antitumor and antiangiogenic properties. Cayman’s 2-Methoxyestradiol ELISA is a competitive assay that can…