Assay Kits

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  • Cyclic AMP Select ELISA Kit

    Cayman Chemical

    Adenosine 3',5' cyclic monophosphate (cAMP) is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized…

  • Cayman’s MTT Proliferation Assay provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells and…

  • Aconitase Assay Kit

    Cayman Chemical

    Aconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated…

  • This DyLight™ Multiplex 488/800 Duo Western Blot Kit contains: Goat anti-Mouse IgG (H+L) DyLight™ 488; Goat anti-Rabbit IgG (H+L) DyLight™ 800; Opal pre-stained protein standard; Wash buffer (10X TTBS); Blocking buffer (2x) and incubation box. This kit is suitable for fluorescent western blotting,…

  • ELECTRODES, ELECTROLYSIS APPARATUS

    United Scientific Supplies

    The Hoffman Electrolysis Apparatus is used for the qualitative and quantitative study of the electrolytic decomposition of electrolytes such as water and hydrochloric acid. Included platinum electrodes are also sold separately. Includes activity guide. Shipping weight: 8 lbs., dimensions:…

  • MP Biomedicals' Free Fatty Acid Test Kits are designed to measure free fatty acid content in any liquid or solid sample containing oil/fat. MP Biomedicals' Free Fatty Acid Test Kit contains all reagents and calibrators necessary to run samples for percent free fatty acid. A control is…

  • CB-X™ Protein Assay

    G-Biosciences

    Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers…

  • CB™ Protein Assay

    G-Biosciences

    An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color…

  • Assay Strips and Holders

    G-Biosciences

    Assay Strips and Holders: 8-well strips of 200ml flat bottomed wells. Ideal for ELISA assays. Cost effective as individual strips can be used as opposed to a whole 96-well plate. Each holder holds 12 strips, sold separately.

  • Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml rat NGAL standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero…

  • For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Sensitive. Detection limit of 0.10 mg/dL (8 μM) creatinine. Can be automated as a high-throughput assay for thousands of samples per day. Method: OD510 nm. Samples: Urine, serum,…

  • Corticosterone ELISA Kit

    Cayman Chemical

    Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (adrenocorticotropic hormone), and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress. Plasma corticosterone…

  • Mouse IgG ELISA Kit

    Cayman Chemical

    Immunoglobulin IgG provides the majority of antibody-based immunity against invading pathogens and plays a significant role in the pathology of auto-immune diseases. Most mouse monoclonal antibodies are of the IgG isotype. Because mice are widely used as a research model for studies of the immune…

  • The CytoScan™ LDH Cytotoxicity Assay is a colorimetric method of assaying cellular cytotoxicity and is an alternative to 51 Cr release cytotoxicity assays. The kit combines the advantages of reliability and simple evaluation, characteristic of radioisotope release assays, with the…

  • UltraPure Water

    Cayman Chemical

    Water used for the preparation of EIA reagents and buffers.

  • The thioredoxin (Trx) system is the major protein disulfide reductase in cells and comprises Trx, Trx reductase (TrxR), and NADPH. Trx systems are ubiquitous and are critical for cell viability, proliferation, activation of transcription factors, and control of intracellular redox states. The…

  • HTS for inhibitor screening and evaluation of acetylcholinesterase inhibitors. Fast and sensitive. Linear detection range 10 to 600 U/L for a 10 minute reaction at 37°C. Homogeneous "mix-incubate-measure" type assay. Can be readily automated for processing tens of…

  • Cayman’s SOD Assay is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in a convenient 96-well…

  • Endothelin ELISA Kit

    Cayman Chemical

    The ET peptide family consists of three isoforms, ET-1 (corresponding to the initially isolated and most predominant isoform), ET-2, and ET-3. ET-1 is a 21 amino acid peptide and is one of the most potent vasoconstrictors currently known. ET-2 displays similar pharmacology to ET-1, whereas ET-3 is…

  • Use Immuno-chemicals’ Anti-DYKDDDDK (FLAG®) Chemiluminescent Kit for Western Blotting for detection of FLAG® -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed…

  • Annexin V FITC Assay Kit

    Cayman Chemical

    One of the hallmarks of the early stages of apoptosis is the redistribution of membrane phospholipids such as phosphatidylserine and phosphatidylethanolamine from the inner to outer leaflet of the membrane bilayer where they are exposed on the cell surface. Externalization of phosphatidylserine…

  • MINI ELECTROLYSIS DEVICE

    United Scientific Supplies

    This easy to set up, inexpensive device was designed by teachers in order to collect the correct proportions of hydrogen and oxygen from the electrolysis of water. Its unique design helps correct issues such as falling test tubes perched over unsteady electrodes and gases escaping due to poor…

  • Chemical Properties Form: Liquid Applications Contains two separate solutions for 500 estimations of proteins Notes Solution A: Bicinchonic acid and tartrate in an alkaline carbonate buffer; Solution B: 4% w/v cupric sulfate Other References …

  • β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol…

  • Better Bradford Protein Assay Kit: The Better Bradford Protein Assay Kit is a quick and ready-to-use coomassie-binding, colorimetric method for total protein quantitation. This modification of the well-known Bradford method greatly reduces the tendency of coomassie reagents to give nonlinear…

  • For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate. High sensitivity and wide linear range. Use 5 μL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L. Homogeneous and simple procedure. Simple…

  • Cayman’s Renin Inhibitor Screening Assay provides a convenient assay in a 96-well format for evaluating human renin inhibitors. The assay utilizes a renin-based synthetic peptide substrate which incorporates the fluorophore EDANS at one end and an EDAN-quenching molecule (Dabcyl) at the other…

  • Vitamin D ELISA Kit

    Cayman Chemical

    The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D3. Vitamin D3 is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D3 (25(OH)D3).…

  • BCA Protein Assay Kit

    Bio Basic Inc.

    BCA Protein Assay Kit: The BCA Protein Assay kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium (the…

  • 30% H2O2 Solution reagent is specifically designed for the use in Rockland MaxTag™ Western Blot Kit.

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