When agarose is placed in a buffer such as TAE (Tris/Acetate/EDTA) or TBE (Tris/Borate/EDTA), it is generally insoluble. However, when this agarose solution is heated, the agarose particles become hydrated and thus go into solution. This hydration process is time-dependent, and different types of…

When agarose is placed in a buffer such as TAE (Tris/Acetate/EDTA) or TBE (Tris/Borate/EDTA), it is generally insoluble. However, when this agarose solution is heated, the agarose particles become hydrated and thus go into solution. This hydration process is time-dependent, and different types of…