Affinity Other Media

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G-Biosciences Affinity Other Media

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  • Concanavalin A (Con A) Agarose

    G-Biosciences

    Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean). Con A is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds…

  • Immobilized Biotin

    G-Biosciences

    Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose. Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordinary binding…

  • Immobilized Protein A/G Resin

    G-Biosciences

    Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Protein A/G binds well to IgG subclasses but does not bind…

  • Glutathione Resin

    G-Biosciences

    Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.…

  • Amine Magnetic Beads

    G-Biosciences

    G-Biosciences’ Amine Magnetic Beads are 1µm, uniformmagnetic beads with an amine (-NH2) surface functional group. The magnetic beads consist of a single-crystal Fe3O4 sphere core and dextran coating layer. Through chemical modification of dextran, the…

  • Epoxy-Activated Agarose (Dry Form)

    G-Biosciences

    Epoxy-Activated Agarose is preactivated resin used for affinity chromatography. Epoxy-Activated Agarose comprise of high density epoxy groups that form covalent bonds with ligand s containing amine groups, thiol or hydroxyl groups. Epoxide-Acivated Agarose is prepared by immobilization of…

  • Nickel Chelating Resin

    G-Biosciences

    Immobilized metal affinity chromatography (IMAC) resin utilizing nickel (Ni 2+ ) for the purification of 6x histidine tagged proteins. This resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked…

  • Immobilized Jacalin

    G-Biosciences

    Jacalin, or Artocarpus integrifolia lectin, is a tetrameric two-chain lectin with a molecular weight of 66kDa.Jacalin is a α-D-galactose binding lectin purified from jack-fruit ( Artocarpus integrifolia ) seeds. Features Preparing Human IgA free of contaminating IgG …

  • Thiophilic Resin

    G-Biosciences

    Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al 1 in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity…

  • Immobilized p-Aminophenyl Phosphoryl Choline

    G-Biosciences

    G-Biosciences' Immobilized p -Aminophenyl Phosphoryl Choline consists of a phosphoryl choline covalently linked to beaded agarose and is designed for the purification of C-reactive protein from plasma, ascites and other biological fluids. CRP, C-reactive protein, is a pentameric…

  • Boronate Resin

    G-Biosciences

    Our Boronate resin is designed for the isolation ofribonucleotide and oligonucleotide RNA. The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups. Mechanism : The boronic…

  • N-Acetyl-D-Glucosamine Agarose

    G-Biosciences

    Lectins are proteins or glycoproteins that have at least one noncatalytic domain that binds specifically and reversibly to monosaccharides or oligosaccharides. Lectins are important as they are found in applications in science, medicine, agriculture, pharmaceutical companies, biotechnology and…

  • HOOK™ Agarose Coupling Kit (Amine Reactive)

    G-Biosciences

    An important tool in research is the generation of peptide and protein affinity columns for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offersa complete kit for the coupling of peptides and proteins to agarose through their…

  • Immobilized Soybean Trypsin Inhibitor

    G-Biosciences

    G-Biosciences Immobilized Soybean Trypsin Inhibitor (STI) resin is designed for the efficient removal of trypsin, chymotrypsin and elastase proteases from protein digests. The action of the Immobilized STI resin will stop enzymatic reactions, in addition to removing the proteases and simplifying…

  • CDI Amine Reactive Agarose

    G-Biosciences

    G-Biosciences CDI Amine Reactive Agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates. The activation of the resin occurs in solvent and to maintain its activity the resin is supplied in acetone to prevent…

  • Carboxyl Coupling Resin (Immobilized DADPA (Diaminodipropylamine))

    G-Biosciences

    The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. This resin is also termedas EAH-Agarose. Molecules, including proteins, peptides and nucleic acids, are covalently…

  • Ni-NTA Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Ni-NTA Resin is specifically designed for the purification of…

  • Copper Chelating Resin

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. The Copper Chelating Resin is specifically designed for the…

  • Immobilized Avidin

    G-Biosciences

    Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10 15 M -1 ). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only…

  • CNBr-Activated Agarose (Dry Form)

    G-Biosciences

    CNBr-Activated Agarose is pre-activated high capacity resinused for covalent coupling of antibodies and large protein molecules for affinity chromatography. Cyanogen bromide reacts with hydroxyl groups of resin to form cyanate esters which inturn reacts with primary amine ligand s to form…

  • Sulfhydryl Immobilization Kit for Proteins

    G-Biosciences

    The Sulfhydryl Immobilization Kit for Proteins is a complete kit designed for the simple and efficient coupling of proteins to a solid agarose support. The Sulfhydryl Coupling Resin Columns utilizes iodoacetyl groups that specifically react with free sulfhydryls to form covalent, permanent…

  • NHS-Activated Agarose (Dry Form)

    G-Biosciences

    NHS-Activated Agarose consists of 4% cross-linked agarose that has been activated by the addition of a reactive NHS (N-hydroxysuccinimide) group. The NHS group forms covalent, chemically stable amide bonds with ligand s that contain primary amines. The NHS-Activated Agarose also contains a spacer…

  • ECH-Agarose, 15mL

    G-Biosciences

    ECH-Agarose contains carboxyl functional group for coupling of affinity ligand s via their amine groups for affinity chromatography. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydrophilic spacer arm. Amine…

  • Immobilized O-Phosphorylethanolamine

    G-Biosciences

    G-Biosciences Immobilized O-Phosphorylethanolamine is used in purification of calcium binding C-reactive proteins such as canine CRP and human CRP. Immobilized O-Phosphorylethanolamine can also be used as substitute for p-Amino Phosphoryl Choline Agarose which is used in purification of…

  • Immobilized Amino Acids

    G-Biosciences

    G-Biosciences Immobilized Amino Acids are prepared by coupling amino acid to preactivated 6% cross-linked agarose. The covalent coupling to agarose (resin) is achieved by cross-linking amino group of the amino acid to the preactivated resin. Features: Amino acid coupled to 6%…

  • Immobilized Monomeric Avidin

    G-Biosciences

    G-Biosciences Immobilized Monomeric Avidin Resin is designed for the simple affinity chromatography purifications of proteins, antibodies and other molecules with a biotin tag. The resin consists of monomeric subunits of avidin covalently coupled to 6% cross-linked agarose, offering a stable,…

  • Immobilized Reductant

    G-Biosciences

    Reducing agents are used in the reduction of disulfide bonds of proteins and peptides. It is often necessary to remove the reducing agents from the protein/peptide solutions to prevent them from interfering with subsequent procedures. However, for small proteins, and particularly peptides, it is…

  • Immobilized Ficin

    G-Biosciences

    Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason.Ficin is typically used to cleave mouse IgG1 as this are difficult to cleave with papain and pepsin .In…

  • G-Sep™ Ni IDA Agarose Fast Flow

    G-Biosciences

    Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.G-Sep™ Ni IDA Agarose Fast Flow is specifically designed for the…

  • Immobilized Imminobiotin

    G-Biosciences

    Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers. The…

  • Calmodulin Resin

    G-Biosciences

    Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1mg calmodulin/ml resin. …

  • p-Aminobenzamidine Agarose

    G-Biosciences

    ρ-Aminobenzamidine Agarose primary application is for the removal and /or purification of trypsin-like proteases.ρ-aminobenzamidine (PAB) is a synthetic inhibitor of trypsin-like proteases and has been covalently coupled to 6% crosslinked agarose. ρ-aminobenzamidine agaroses have…

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