Western Blotting and ELISA Chemicals
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AzureSpectra RG rb650/ms550 Western Kit, with Fluorescent Block
Azure BiosystemsKit contains materials for 10 Western blots – LF PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra rb650/ms550
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Low Fluorescence Western Membrane (LF PVDF) 0.45um (10x15)
Azure BiosystemsPre-cut LF PVDF membrane (10x15 cm vertical gels); 10 sheets
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Low Fluorescence Western Membrane (LF PVDF) 0.45 um (13x18)
Azure BiosystemsPre-cut LF PVDF membrane (13x18 cm vertical gels); 10 sheets
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femtoLUCENT™ PLUS AP Kit
G-BiosciencesThe femtoLUCENT™ PLUS-AP kit is based on alkaline phosphatase (AP) chemiluminescence reaction. The chemiluminescence light emission can be recorded by a short exposure to blue-light sensitive autoradiography films. The femtoLUCENT™ PLUS-AP kit allows for detection of femtogram quantity…
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Azure Fluorescent Blot Blocking Buffer
Azure BiosystemsBlocking solution for fluorescent Western blot, 1x; 500 ml
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Quenching Sheets
Azure Biosystems10 background quenching sheets for use with fluorescent or chemiluminescent samples (9x12.5 cm)
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Well-Coated™ Glutathione Plates
G-BiosciencesWell-Coated™ Glutathione plates are designed to specifically bind GST (Glutathione S-Transferase) tagged proteins and peptides. The plates have immobilized glutathione and can be used for the isolation of GST-tagged proteins direct from bacterial lysates and then used for subsequent ELISA…
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AzureSpectra Labeling Kit – for Western Blot – 700
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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Protein Sample Loading Buffers
AdvanstaPre-mixed, standard Laemmli loading buffers for protein electrophoresis These ready-to-use Laemmli loading buffers contain Bromophenol blue to enable visualization of the dye front and monitoring of the progress of electrophoresis. Choose from reducing or non-reducing buffers, each supplied…
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Well-Coated™ Antibody Plates
G-BiosciencesWell-Coated ™ Antibody plates are designed to specifically bind either mouse or rabbit IgG making them suitable for binding assays using low quantities of antibodies or antibodies that denature on direct binding to polystyrene plates. Another advantage of using the Well-Coated ™…
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Nitrocellulose Membranes
G-BiosciencesFor user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. …
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Swift Western Diluent
G-BiosciencesThe Swift™ Western Diluent is a unique solution that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes. Swift™ Western Diluent generates comparable result to traditional Western blotting procedures and other commercial…
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AzureSpectra Labeling Kit – for Western Blot – 650
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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FLASHBlot™ Transfer Buffers
AdvanstaEnhanced protein transfer for improved sensitivity Achieve improved detection of low-abundance and posttranslationally modified proteins with Advansta's proprietary FLASHBlot Transfer Buffer. High-efficiency protein transfer and increased protein retention on the membrane add up to more…
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femtoCHROMO™-HRP Kit
G-Biosciencesfemto CHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein band s with low background staining. To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X)…
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Radiance Plus
Azure BiosystemsFemtogram HRP substrate (150 ml kit); contains substrate for 1,500 cm2 membrane
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Azure ChemiWriter ECL
Azure BiosystemsChemiluminescent marker that enables to write or draw on transfer membranes, resulting in a chemiluminescent signal on markings once substrate is added; 1 piece
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Well-Coated™ Protein A/G Plates
G-BiosciencesWell-Coated ™ Protein A/G plates are designed to bind the constant (Fc) region of immunoglobulins ensuring that the antigen binding domain of the antibody is orientated away from the plate, offering maximum exposure of the binding site. Protein A-G contains 4 binding sites from protein A and…
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Incubation Trays
AdvanstaConvenient, easy to use trays for staining and washing gels and membranes These specially designed incubation trays are ideal for staining and washing electrophoresis gels and membranes. Chose from three designs, all available in a range of sizes: Opaque – for light-sensitive…
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AzureSpectra Labeling Kit – for Western Blot – 490
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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Superior™ Blocking Buffer
G-BiosciencesSuperior ™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. Superior ™ Blocking Buffer is ideal for a high signal to background ratio in most…
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NBT/BCIP Stain Kit
Bio Basic Inc.NBT/BCIP Stain Kit: NBT and BCIP refers to Nitro-Blue-Tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate respectively. When mixed together, BCIP is hydrolyzed and reacts with NBT, which then react with O2, forming visible purple precipitates in the presence of AP (alkaline phosphatase). The…
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Trastuzumab Elisa Kit
Lab BioreagentsTrastuzumab ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of Trastuzumab with high sensitivity and specificity in serum and plasma. Kit Performance Sensitivity: 10 ng/mL Assay Range: 10 – 300 ng/mL Cross-reactivity: There is no cross reaction…
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Low Fluorescence Western Membrane (LF PVDF) 0.45um (9x7)
Azure BiosystemsPre-cut LF PVDF membrane (9x7 cm mini vertical gels); 10 sheets
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Nivolumab Elisa Kit
Lab BioreagentsNivolumab ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of Nivolumab with high sensitivity and specificity in serum and plasma. Kit Performance Sensitivity: 5 ng/mL Assay Range: 5 – 500 ng/mL Cross-reactivity: There is no cross reaction with…
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BLOK™ BSA
G-BiosciencesProtein solutions are used for blocking excess binding sites in ELISA, blotting, immunohistochemistry and other immunochemical applications. Each blocking solution will impart certain characteristics on the assay system. Our BLOK™ BSA is a 10% solution of BSA (Bovine Serum Albumin) …
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AzureSpectra Labeling Kit – for Western Blot – 550
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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Nitrocellulose Transfer Membrane 0.22um (8x10)
Azure BiosystemsPre-cut membrane (8x10 cm mini vertical gels, ideal for smaller proteins); 10 sheets
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Well-Coated™ Biotin Plates
G-BiosciencesWell-Coated ™ Biotin plates are designed to specifically bind avidin, streptavidin or Neutravidin™ conjugated molecules, including enzyme conjugates. Biotin exhibits an extraordinary binding affinity for avidin (Ka=10 15 M -1 ) and streptavidin (Ka=10 15 M -1 ). Biotin and avidin…
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Nitrocellulose Transfer Membrane 0.45um (8x10)
Azure BiosystemsPre-cut membrane (8x10 cm mini vertical gels, ideal for larger proteins); 10 sheets
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SWIFT™ Transfer Pads
G-BiosciencesWestern blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amount of time taken to transfer the proteins to a protein binding membrane; the second is the variable efficiency of the transfer and the third is problems…
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Azure Protein-Free Blot Blocking Buffer
Azure BiosystemsProtein-free blocking solution for Western blot, 5x concentrate; 200 ml