2D Sample Preparation

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2D Sample Preparation

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  • Dithiothreitol

    GE Healthcare

    Reducing agents are frequently included in the sample solution to break any disulfide bonds present and to maintain all proteins in their fully reduced state. The most commonly used reductant is dithiothreitol (DTT).

  • DeStreak Rehydration Solution

    GE Healthcare

    DeStreak Rehydration Solution Improves reproducibility and quality of 2-D gels by preventing streaking. Eliminates extra spots caused by nonspecific oxidation of proteins. Stabilizes proteins throughout entire 2-D runs, ensuring simplified, well-resolved protein patterns. Highly suitable for…

  • DeStreak Reagent

    GE Healthcare

    DeStreak Reagent Improves reproducibility and quality of 2-D gels by preventing streaking. Eliminates extra spots caused by nonspecific oxidation of proteins. Stabilizes proteins throughout entire 2-D runs, ensuring simplified, well-resolved protein patterns. Highly suitable for both…

  • IPG-Strip Equilibration Buffer

    G-Biosciences

    Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer. Eliminates worries about urea induced protein carbamylation or waste of unused reagents, as fresh and ready-to-use reagents are prepared every time. The Equilibration Buffer…

  • InGel™ Array

    G-Biosciences

    A 96-well format kit suitable for processing larger numbers of protein spots concurrently and compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste. Excise protein spots and transfer to the supplied proteomic grade titer plate,…

  • InGel™ Blue

    G-Biosciences

    The InGel™ Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie or fluorescent stained protein spots/band s. The kit has all the…

  • Pep-Extract™

    G-Biosciences

    Following in gel digestion of proteins with proteases, the addition of Pep-Extract ™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis, without any additional clean up. Supplied with enough reagents for >500…

  • InGel™ Silver

    G-Biosciences

    A reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry. The protein spots are first excised from the gel and transferred to a proteomic grade tube. Silver stained gel pieces are washed with…

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