Total RNA Minipreps from cultured cells and blood samples
The Total RNA MINI and MAXI Kits (blood and cultured cell) are specially designed for purification of total RNA from fresh whole human blood and cultured cells. This method uses detergents and a chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass-fiber matrix of the spin column. Once any contaminates have been removed following the wash step, the purified RNA is eluted by RNase-free water. The entire procedure can be completed within 30 minutes (MINI) and 60 minutes (MAXI), and the purified RNA is ready for RT-PCR, Northern Blotting, Primer Extension, and cDNA library construction.