Fast Genomic DNA Preparations from Blood Samples
The Genomic DNA MINI and MAXI (Blood/Cultured Cell) Kits provide a fast and economical method for the purification of total DNA (including genomic, mitochondrial, and viral DNA) from fresh whole blood, plasma, serum, buffy coat, other bodily fluids, lymphocytes, bacteria, and cultured cells. The method uses a chaotropic salt to lyse cells and degrade proteins. The DNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminates have been removed, the purified DNA is eluted by a low salt elution buffer or water. The entire procedure can be completed in 40 minutes (MINI) or 60 minutes (MAXI) without the use of phenol extraction or alcohol precipitation.